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2006 Vol. 26, No. 03 Published: 2006-03-26

光谱学与光谱分析

	385	Applications of Terahertz Pulse in the DNA Molecule
		WANG Xiao-hong,ZHANG Liang-liang,HU Ying^*,ZHANG Cun-lin
		Terahertz(THz) radiation is a newly developed coherent far infrared source, which has been applied in many research fields, especially medical application and biological application. In the present paper, the mechanism and methods of its generation and detection are introduced. The properties and its uniqueness in applications to biological researches and DNA molecular spectroscopy are discussed. A brief review is given to the latest progress in THz spectroscopy of DNA samples, and the prospect of the research in this crossdiscipline is presented.
		2006 Vol. 26 (03): 385-391 [Abstract] ( 1721 ) PDF (918 KB) ( 401 )

	392	Concentration Calibration Method of Ambient Trace-Gas Monitoring with Tunable Diode Laser Absorption Spectroscopy
		KAN Rui-feng, LIU Wen-qing, ZHANG Yu-jun, LIU Jian-guo, WANG Min, GAO Shan-hu, CHEN Jun
		Tunable diode laser absorption spectroscopy (TDLAS) is a new method to detect trace-gas qualitatively or quantificationally based on the scan characteristic of the diode laser used to obtain the absorption spectroscopy in the characteristic absorption region. It needs to be combined with a long absorption path in the ambient trace-gas measurements. TDLAS is a new trace gas detective method developed with the combination of a tunable diode laser source and a long absorption path; it has significant advantages not only in the sensitivity but also in rapidity of response. It has been widely used in many atmospheric trace-gases detection, ground trace-gas detection and, gas leakage detection. On-line calibrating is necessary to most trace gas monitor, and in the present paper the authors introduced a simple and accurate method, analyzed it in the theory, and proved it’s feasibility in the experiment.
		2006 Vol. 26 (03): 392-395 [Abstract] ( 251 ) PDF (497 KB) ( 474 )

	396	Study on Photoluminescence Spectra of Cd_1-xMn_xTe/CdTe Diluted Semiconductor Superlattices with High Mn Compositions
		CHEN Chen-jia¹,WANG Xue-zhong¹,Bellani V²,Stella A²
		The electronic properties of Cd_1-xMn_xTe/CdTe semiconductor superlattices (SLs) with high Mn concentrations (x up to 0.8) were studied by means of low and room temperature photoluminescence(PL). The samples were grown by MBE on GaAs substrates with CdTe buffers. PL allowed the investigation of the optical emission from the fundamental 11H excitonic transition, revealing the high quantum efficiency and quality of the SLs. The temperature dependence of the excitonic PL energy position and excitonic line-shape broadening were studied. An exciton-longitudinal optical phonon couple model can be used to explain the experimental results very well. Photoreflectance (PR) spectra were also performed in order to compare with the authors’ photoluminescence results. The combined use of PL and PR allowed a full understanding of the electronic states and optical emission characteristics of the authors’ SLs. The authors’ results indicate that high quality Cd_1-xMn_xTe/CdTe SLs with high Mn concentrations can be grown using MBE on GaAs substrates. The possibility to have magnetic semiconductor nano-structures opens the way to applications in the field of spintronics toward devices based on new concepts.
		2006 Vol. 26 (03): 396-398 [Abstract] ( 256 ) PDF (385 KB) ( 384 )

	399	Photostimulated Luminescence and Optical Storage of BaLiF₃:Eu²⁺
		WANG Xin-zi, WANG Yong-sheng, SUN Li, XU Zheng
		BaLiF₃:Eu²⁺ was prepared by high temperature solid state reaction. The peaks of photoluminescence spectrum and photostimulated luminescence (PSL) spectrum are both at 410 nm which are attributed to the 5d-4f emission of Eu2+. The photostimulated luminescence excitation spectrum has its maximum at about 660 nm. Therefore, He-Ne laser is suitable for the reading-out process. The mechanism of PSL was also studied according to the characters of the spectra in the present paper. The PSL decay property of the glass ceramics was investigated, and it was shown that the storage information is easy to be erased. BaLiF₃:Eu²⁺ has good properties of PSL and a promising future.
		2006 Vol. 26 (03): 399-402 [Abstract] ( 320 ) PDF (438 KB) ( 355 )

	403	Influence of Erbium Ion Concentration on Judd-Ofelt Parameters of Er³⁺-Doped Tellurite Glass
		ZHOU Gang^{1, 2},DAI Shi-xun¹,YU Chun-lei^{1, 2},ZHANG Jun-jie¹,HU Li-li¹,JIANG Zhong-hong¹
		Er³⁺-doped tellurite glasses with four different concentrations were fabricated, and the oscillator strength of Er³⁺ in the tellurite glasses were calculated through the absorption spectra of the glasses. The Judd-Ofelt intensity parameter Ω_i,spontaneous transition probability A,fluorescence branching ratio β,and radiative lifetime τ_rad of Er³⁺ were calculated on the basis of Judd-Ofelt theory，and the effect of the erbium ion concentration on the above optical parameters was also discussed. The fluorescence spectra of Er³⁺: ⁴I_13/2→⁴I_15/2 transition and the lifetime of Er³⁺: ⁴I_13/2 level of the samples were measured. The stimulated emission cross-section of ⁴I_13/2→⁴I_15/2 transition of the samples was finally calculated by using McCumber theory. The results show that with the increase in the Er³⁺ concentration, the oscillator strength and spontaneous transition probability A of Er³⁺ increase, while the fluorescence branching ratio β of Er³⁺ shows little difference. The stimulated emission cross-section of Er³⁺: ⁴I_13/2→⁴I_15/2 transition of the samples changes slightly with the increase in the Er³⁺ concentration. All the fluorescence effective line widths for the four different Er³⁺ concentration samples are nearly 50 nm.
		2006 Vol. 26 (03): 403-407 [Abstract] ( 1687 ) PDF (510 KB) ( 419 )

	408	The Survey and Its Interpretation for Interstellar OH and H₂O Masers
		LIU Han-peng¹,Forster J R²,SUN Jin³
		A statistics of sky survey for interstellar H₂O and OH masers is presented. These masers can be classified into 3 categories. The total number of sources in each category is of the same order of magnitude, and they can maintain -10⁵ years. But there are fewer than half masers with detectable HⅡ regions. An improved radiative pumping mechanism is proposed, and it overcame the known drawback of former radiative models. The observationally statistical results can be interpreted by both the new mechanism and the evolutional model with gravitational force of star responsible for the HⅡ region.
		2006 Vol. 26 (03): 408-410 [Abstract] ( 408 ) PDF (383 KB) ( 345 )

	411	Study of Nanostructures and Reflective Spectra of Porous Alumina Films
		ZHANG Dong-xian, ZHANG Hai-jun, LIN Xiao-feng, HE Yu-lin
		Several chips of porous alumina films with different nanostructures and scales were prepared by using the method of anodic oxidation. An atomic force microscope (AFM) was employed to image the surfaces of alumina films, and the corresponding reflective spectra of them were acquired with a spectrometer. The experiments show that the alumina films with average pore diameters of 26 and 39 nm feature particular reflective spectra, each consisting of a periodic distribution of peaks and valleys within the wavelength range from 500 nm to 1 000 nm. The density or the number of peaks and valleys was determined by the scales of nanostructures. The present paper discussed the relationship between the scale of nanostructures and the distribution of reflective spectra. Some satisfying explanations for them were presented.
		2006 Vol. 26 (03): 411-414 [Abstract] ( 1103 ) PDF (708 KB) ( 429 )

	415	Study of Luminescence Properties of Nano-Size ZnO Embedded in SiO₂ Layer Grown by Radio-Frequency Magnetron Sputtering
		SHANG Hong-kai, ZHANG Xi-qing^*,YAO Zhi-gang, TENG Xiao-ying, WANG Yong-sheng, HUANG Shi-hua
		Nano-size ZnO embedded in SiO₂ layers were grown by radio-frequency magnetron sputtering. Absorption spectra and PL spectra were employed to study the optical character of the samples at room temperature. Absorption spectra blue-shifted when the size of nano-meter ZnO decreased, which indicated that quantum size effect became stronger with decreasing the size of ZnO. PL spectra show two peaks at about 387 and 441 nm, respectively. It was concluded that the UV emission originates from the radiative recombination of free-exciton, and the blue emission is due to the electron transition from donor levels of oxygen vacancies to the top of valence band. The origin of the two peaks is demonstrated by time-resolved spectra and luminescence decay curve.
		2006 Vol. 26 (03): 415-417 [Abstract] ( 1631 ) PDF (463 KB) ( 398 )

	418	Study on Photoluminescence of 8-Hydroxyquinaldine Gallium Acrylate and Methyl Methacrylate Copolymer
		XIAO Zun-hong
		A new copolymer of 8-hydroxyquinaldine gallium acrylate and methyl methacrylate was prepared. The composition and properties of the copolymer were characterized by FTIR, UV-Vis, DSC, TG and fluorescence spectra. A strong blue-green photoluminescence, with the peak at 496 nm, was observed for the copolymer/CHCl₃ solution. Effect of monomer ratio and concentration on the photoluminescence property of the copolymer was investigated. The results showed that the maximum fluorescence intensity was attained when Ga(Mq)₂A∶MMA was 1∶20, the maximum excitation wavelength is approximately 263 nm when the concentration of the copolymer is under 2 g·L^-1,but the maximum excitation wavelength exceeds 365 nm when the concentration is above 4 g·L^-1. The copolymer was soluble in chloroform, acetone, and DMF at 25 ℃, so it could be easier to prepare electroluminescent device by spin-coat technology.
		2006 Vol. 26 (03): 418-421 [Abstract] ( 1130 ) PDF (508 KB) ( 368 )

	422	Study of Methanol Adsorption on Zirconia Polymorphs by FTIR
		MA Zhong-yi^{1, 2},DONG Qing-nian¹,YANG Cheng¹,WEI Wei¹,CHEN Jian-gang¹,SUN Yu-han¹
		The influence of zirconia polymorphs on methanol adsorption was investigated by FTIR technique. One terminal and two types of bridged methoxyl were formed on the am-ZrO₂ and t-ZrO₂ samples, while another tribridged methoxyl species was detected on the m-ZrO₂ sample. During the formation of methoxyl, bridged hydroxyl species on the am-ZrO₂ and m-ZrO₂ was more active, while the terminal one on the t-ZrO₂ sample was more active. The methoxyl was oxygenated by surface oxygen ions to be formate and further to be carbonate on the m-ZrO₂ and am-ZrO₂ samples. But for t-ZrO₂,methoxyl could be directly oxygenated to be carbonate at RT, which implied that the surface oxygen ions on t-ZrO₂ were more active than those on the two other samples.
		2006 Vol. 26 (03): 422-425 [Abstract] ( 484 ) PDF (468 KB) ( 420 )

	426	Real-Time Analysis of Soil Moisture, Soil Organic Matter, and Soil Total Nitrogen with NIR Spectra
		SUN Jian-ying¹,LI Min-zan^1*,ZHENG Li-hua¹,HU Yong-guang^{1, 2},ZHANG Xi-jie¹
		The grey-brown alluvial soil in northern china was selected as research object, and the feasibility and possibility of real-time analyzing soil parameter with NIR spectroscopic techniques were explored. One hundred fifty samples were collected from a winter wheat farm. NIR absorbance spectra were rapidly measured under their original conditions by a Nicolet Antaris FT-NIR analyzer. Three soil parameters, namely soil moisture, SOM (soil organic mater) and TN (total nitrogen) content, were analyzed. For soil moisture content, a linear regression model was available, using 1 920 nm wavelength with correlation coefficient of 0.937, so that the results obtained could be directly used to real-time evaluate soil moisture. SOM content and TN content were estimated with a multiple linear regression model, 1 870 and 1 378 nm wavelengths were selected in the SOM estimate model, and 2 262 and 1 888 nm wavelengths were selected in the TN estimate model. The results showed that soil SOM and TN contents can be evaluated by using NIR absorbance spectra of soil samples.
		2006 Vol. 26 (03): 426-429 [Abstract] ( 1664 ) PDF (502 KB) ( 444 )

	430	Error Analysis and Calibration Method of Complex Spectrum of Fourier Transform Infrared Spectrometer
		ZHANG Tian-shu, LIU Wen-qing, GAO Min-guang, LU Yi-huai, LIU Jian-guo, XU Liang, ZHU Jun
		The phase shift in complex spectrum of Fourier transform infrared (FTIR) spectrometer was discussed. A rigorous derivation showed the relation between the phase shift and complex spectrum. The reason for the radiation offset in FTIR measurement was analysed. The present article presents a calibration method of FTIR complex spectrum, which can adjust the radiation offset. The comparison between experiment data and theoretical computation showed that the method is precise.
		2006 Vol. 26 (03): 430-433 [Abstract] ( 1490 ) PDF (426 KB) ( 402 )

	434	Quantitative Determination of Imidacloprid by Infrared Absorption Spectrometry
		MA Guo-xin^{1, 2},WANG Cheng-long^1*,FAN Duo-wang¹,XING Da³,QIAN Long¹,WANG Jin-hui¹,LIU Song-hao⁴
		The content of imidacloprid in pesticide is usually measured by high-performance liquid chromatography, which is precise but time-consuming. In the present work the content of imidacloprid in pesticide was measured by infrared spectroscopy with pressing potassium bromide troche(KBr). A comparison of infrared spectra between analytic imidacloprid and commercial pesticide showed that the absorption peak of imidacloprid at 939.2 cm^-1 is independent and can be used as the characteristic absorption peak for quantitative determination. The area from 947 to 925.8 cm^-1 for the infrared spectra of imidacloprid and its content meet the linear equation: Area=1.366 5×10^-1+2.37×10^-2×c,r=0.999 53. Finally, the authors could draw a conclusion that the method of NIR, instead of routine lab analysis, is feasible for the determination of imidacloprid in pesticide.
		2006 Vol. 26 (03): 434-437 [Abstract] ( 1189 ) PDF (433 KB) ( 442 )

	438	An Analysis of Sweet Pepper Seeds with Space Flight Mutagenesis by FTIR
		YANG Qun¹,WANG Yi-lin¹,YANG Ai-ming²,YANG De³
		The first-time application of FTIR to analyzing and comparing two sweet pepper seeds lines of space mutation and two sweet pepper seeds lines bred in system approaches as well as their spectra is presented. The IR spectrum of sweet pepper seeds lines is mainly composed of the band of adsorption of protein and that of carbohydrates. The absorbance of the two sweet pepper seeds lines of space mutation at 2 854，1 652, 1 542 and 1 160-1 062 cm^-1 was enhanced. The peak near 2 854 cm^-1 is attributable to the stretching vibration of CH₂—, The dominating bands near 1 652 and 1 542 cm^-1 were assigned to amide Ⅰ and amide Ⅱ respectively. The absorbance at 1 160 cm^-1 is attributable to the stretching vibration of C—O on the structure of carbohydrates. The major components and the structures remained intact，indicating that the space environment can increase carbohydrates and protein content of sweet pepper seeds lines.
		2006 Vol. 26 (03): 438-440 [Abstract] ( 1898 ) PDF (323 KB) ( 357 )

	441	Remote Passive Detection of Flame Temperature of Solid Propellant Adulterating Nanoparticles
		ZHANG Li-ming¹,ZHANG Lin¹,LI Yan¹,LIU Bing-ping^1，2,WANG Xiao-fei^1，3,WANG Jun-de^1*
		The flame temperature of three kinds of solid propellants was measured by passive remote sensing FTIR with the resolution of 1 cm^-1. These three kinds of solid propellants are adulterate nano-scale metal oxide particles, adulterate normal metal oxide particles，and propellant without any adulterations. The main components of the solid propellant are nitrocellulose and nitroglycerin. The metallic oxides，including 6 nm CuO, 56 nm Fe₂O₃,16 nm NiO，and correspondingly the normal particles，were adulterated into the solid propellants respectively. The flame temperature was calculated through the fine structure of the emission fundamental band of H₂O at 2.75 μm. The results of the flame temperature of the solid propellants adulterating nano-scale CuO, Fe₂O₃ and NiO are 3 089, 3 193 and 3 183 K, respectively. The temperatures of the three kinds of solid propellants were compared, and it was shown that there is no obvious difference in the flame temperature among the three kinds of solid propellants.
		2006 Vol. 26 (03): 441-443 [Abstract] ( 429 ) PDF (286 KB) ( 434 )

	444	Synthesis, Characterization and Antimicrobial Activity of Nano-Fumed Silica Derivative with Quaternary Ammonium Salts
		XU Xia^{1, 2},YANG Qin-huan¹,LEI Wan-xue¹,LI Zheng-jun¹,HE Zhuang-zhi¹,LIN Fang¹,ZHANG Ting-you^1*
		Nano-fumed silica reacted with γ-chlopropyltrimethoxysilane as a coupling agent, and then by quaternization with N,N-dimethyl-n-tetradecylamine, finally the nano-fumed silica derivative with quaternary ammonium salts was obtained. The nano-fumed silica derivative was investigated by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), and Zeta sizer nano series. The antimicrobial properties of the nano-fumed silica derivative against selected microorganisms were tested by the quantitative suspension method. The results revealed that (1) γ-chlopropyltrimethoxysilane can be bound to the surface of the nano-fumed silica. With increasing the amount of γ-chlopropyltrimethoxysilane, the amount of surface hydroxyl groups of nano-fumed silica decreases. (2) The Zeta potential showed that the isoelectric point of the nano-fumed silica and the modified nano-fumed silica is pH 4.8 and pH 10.5, respectively. The shift of the isoelectric point is due to the quaternary ammonium groups. (3) The obtained nano-fumed silica derivative has significant inhibition effect on the growth of microorganisms，and the bactericidal rates in 15 min for E.coil (8099), S. aureus (ATCC6538) and C. albicans(ATCC10231) were 99.99％, 99.99％ and 94.12％, respectively.
		2006 Vol. 26 (03): 444-447 [Abstract] ( 1598 ) PDF (547 KB) ( 403 )

	448	Identification Study of Mongolian Herbal Medicine Rubus Sachalinesis Leveille and Its Forgeries by UV/Fluorescence/IR Spectroscopy
		XU Liang^{1, 2},Suritalatu³,XI Hai-shan¹,BI Kai-shun²,SUN Wurina¹
		Identification study of four species of Mongolian herbal medicine by UV/fluorescence/IR spectroscopy was carried out. Ultrasonic extracts of Mongolian herbal medicine Rubus Sachalinesis Leveille and its substitute materials Sambucus williamsii Hance and Uncaria rhynchophylla (Miq.) Jacks., and its false medicine Cinnamomum cassia Presl by methyl alcohol, petroleum ether and chloroform were obtained respectively, UV/fluorescence spectral scans for the above herbal medicines were taken and the spectra obtained were compared. Besides, the IR spectra obtained from the herbal medicines were compared. It was shown that the absorption peaks of UV spectra of the four species of Mongolian herbal medicine differ from each other when the methyl alcohol, petroleum ether and chloroform were used as extracting solvent, while there is a selectivity for the solvent when fluorescence spectrum was used to differentiate those medicines. Only when the methyl alcohol was used as extracting solvent, there are clear differences among the IR spectra of the four species of medicine. Therefore, three kinds of spectra are available in identifying the four species of herbal medicine. Among them, IR spectroscopy is noted for its more information about the fingerprints of herbal medicines, and being direct and simple, which is one of the methods worth recommending for identifying Mongolian herbal medicine.
		2006 Vol. 26 (03): 448-451 [Abstract] ( 1089 ) PDF (424 KB) ( 377 )

	452	Surface-Enhanced Raman Spectroscopy Study on the Interaction of 4,4’-Bipyridine and Zn Electrode
		GU Ren-ao,SHEN Xiao-ying,CUI Yan,GU Wei
		In the present paper, surface enhanced Raman spectroscopy (SERS) was employed to study the interaction of 4,4’-bipyridine(BiPy) and Zn electrode. The results indicate that BiPy is adsorced with a vertical (or incline) orientation on the Zn electrode via one N atom of BiPy, and the two pyridine rings still remain on the same plane. BiPy has relatively stronger interaction with zinc metal than pyridine. The larger Raman scattering cross section and the existence of the two pyridine rings make it easy for 4,4’-BiPy to have stronger interaction with zinc metal.
		2006 Vol. 26 (03): 452-454 [Abstract] ( 1110 ) PDF (405 KB) ( 451 )

	455	Structural and Property Characteristics of Chinese Fir Wood Charcoal Prepared under Various Conditions
		HUANG Biao¹,CHEN Xue-rong¹,JIANG Mao-sheng¹,GAO Shang-yu²
		By techniques of one-step and two-step carbonization, and capped and uncapped carbonization, the pyrolysis on the thinning wood of Chinese fir, one of the fastest growing tree species in south China, was carried out. With special focus on the variation of Raman spectra and X-ray diffraction analysis of carbonized materials under different carbonization conditions, comprehensive analyses of charcoals were conducted. Results show that, with temperature rising, the graphite-like microcrystal and carbon atoms at the edge of graphite-like microcrystal increase rapidly. As a result, the hexagon carbon plane structure in carbonized material keeps growing. Moreover, the experiments reveal that carbonization condition has great effects on the property and structure of carbonized material, and 700 ℃ is the critical temperature at which the qualitative change of carbonized material occurs. Furthermore, formation and growth of graphite-like microcrystal in carbonized material, two-step method is more beneficial than one-step method, uncapped container carbonization method is better than the capped one, and using air as atmosphere gas is better than using N₂. Samples with longer holding time in the process of carbonization enjoy higher graphitization extent.
		2006 Vol. 26 (03): 455-459 [Abstract] ( 1549 ) PDF (644 KB) ( 420 )

	460	Raman Spectroscopic Study of Human Serum Albumin Interacting with 3-Picolylamine
		GUO Jian-yu¹,SUN Zhen-rong^1*,WU Liang-ping¹,LI Rong-xiu²,WANG Zu-geng¹
		Raman spectra of human serum albumin (HSA) and HSA-3-picolylamine complex were obtained. The spectra indicate the configuration and structural transformations of HSA. The results show that the secondary structure is main α-helix, and the binding of 3-picolylamine doesn’t change the secondary structure. However, the binding changes the configuration of disulfide bonds, transforms a single tryptophan residue from exposed tryptophan residues to hydrophobic environment, and alters the microenvironment of tyrosine.
		2006 Vol. 26 (03): 460-462 [Abstract] ( 1102 ) PDF (308 KB) ( 438 )

	463	Calculation of the SERS Enhancement Factors of Pyridine Molecules Adsorbed on the Substrates of Fe, Co and Ni Using Antenna Resonace Model
		LI Jia-wei^1,2,BAI Ying¹,MO Yu-jun^1*,Wachter P^{3 △}
		The theory of antenna resonance model is a theory advanced in the investigation of the mechanism of surface enhanced Raman scattering. In the present paper, as a representational example the authors have calculated the enhancement factors of the vibrational mode 1 010 cm^-1 of pyridine molecules adsorbed on the metal substrates of Fe, Co and Ni through this model. The dependence of the enhancement factor of Raman scattering on different incident energy at resonance was studied and that on surface roughness of the three metals with the excitation of 0.89 eV was brought forward. As a result, the enhancement factors in visible region are small, but they are rather large (10⁴-10⁷) in infrared region.
		2006 Vol. 26 (03): 463-466 [Abstract] ( 1108 ) PDF (429 KB) ( 403 )

	467	Spectroscopy Research on Cholesterol in Hypercholesterolemia Serum
		LAN Xiu-feng¹,LIU Jian-gang¹,LIU Ying^{1, 2},LUO Xiao-sen¹,TANG Xin-guo³,LU Shi-yue³,NI Xiao-wu^{1, 2*}
		The cholesterol with different concentration in hypercholesterolemia serum was studied by the method of spectroscopy technology. The absorption and fluorescence spectra of normal human serum and hypercholesterolemia serum were obtained respectively; the spectral characteristic of each sample and the difference between two kinds of samples were discussed too. The results indicate that the absorption and fluorescence spectra of hypercholesterolemia serum are different from those of normal human serum. The absorptivity and the fluorescence intensity of hypercholesterolemia serum are both higher than those of normal human serum. Besides, there are new absorptive peaks and new fluorescence peaks in the spectrogram. Thus, the abnormalism of cholesterol in serum can be judged by comparing the absorption and fluorescence spectra. The researches in the present paper provide an experimental foundation for the diagnosis of cholesterol in blood.
		2006 Vol. 26 (03): 467-470 [Abstract] ( 1652 ) PDF (448 KB) ( 398 )

	471	Effect of Sunlight Irradiation on Fluorescence Properties of Dissolved Organic Matter
		FU Ping-qing^{1, 2},WU Feng-chang^1*,LIU Cong-qiang¹,XU Cheng1, WANG Jing^{1, 2},BAI Ying-chen^{1, 2},WANG Li-ying^{1, 2}
		Three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM) was used to investigate the effect of sunlight irradiation on the fluorescence properties of dissolved organic matter (DOM) from Lake Hongfeng and Nanming River waters and a commercial fluka humic acid (FHA). The results show that the DOM samples and FHA fluorescence properties changed under sunlight irradiation. Interestingly, the photodegradation characteristics were different between aquatic DOM and FHA. The fluorescence intensity of the apparent peaks A, B and C of lake and river water DOM decreased with sunlight irradiation. The initial 3DEEM of Fluka HA had only one fluorescence peak at λ_ex/λ_em=275/500 nm, while two fluorescence peaks occurred at λ_ex/λ_em=245/450 nm and 310/450 nm, respectively, after sunlight irradiation. λ_ex and λ_em maxima of DOM decreased during 7 days of sunlight irradiation. Changes in r(A, C) of DOM and FHA with sunlight irradiation time suggest that fluorescence peaks A and C had different fluorescence loss rates, while peak C fluorophores were more susceptible to sunlight irradiation. FHA appeared to be less susceptible to photodegradation，and its r(A, C) remained almost the same before and after sunlight irradiation.
		2006 Vol. 26 (03): 471-474 [Abstract] ( 1612 ) PDF (570 KB) ( 445 )

	475	Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method
		GUO Yu-hua¹,CHEN Xiao-lan¹,ZHANG Ting¹,QU Ling-bo^{1, 2*},ZHAO Yu-fen^{1, 3},YU You-zhu¹
		The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide(EB)as a probe. The result showed that both 7-hydroxy flavone and its phosphate could form non-covalent complexes, but the phosphorylated flavonoid showed higher binding affinity with DNA than 7-hydroflavone did. Experiments demonstrated that the higher the temperature, the lower the slop of quenching curve of DNA-EB in the presence of different amounts of 7-hydroxy flavone and its phosphate. It was confirmed that the combinations of DNA with 7-hydroxy flavone and its phosphate were a single static quenching process. According to the Stern-Volmer equation and Scatchard equation the quenching constants and the intrinsic binding constants of 7-hydroxy flavone and its phosphate were measured respectively, they were K_q₁= 601 L·mol^-1,K_q₂=1 381 L·mol^-1;K₁=2.07×10⁴ L·mol^-1,K₂=3.19×10⁴ L·mol^-1 respectively.
		2006 Vol. 26 (03): 475-479 [Abstract] ( 1042 ) PDF (539 KB) ( 601 )

	480	Study on the Interaction of Lomefloxacin Terbium Complex and Bovine Serum Albumin by Fluorescence Spectra
		HE Hua¹,YE Hai-ying¹,DAI Li¹,JIAO Qing-cai²,CHUONG Pham-Huy³
		Using lomefloxacin-terbium as a fluorescent probe, the interaction of LFLX-Tb³⁺ and bovine serum albumin (BSA) was investigated by fluorescence spectra. The interaction of LFLX and BSA is quite strong. LFLX can affect the conformation of BSA to some degree. Meanwhile, the electrostatic binding of the BSA with Tb³⁺ in LFLX-Tb³⁺ complex could decease the nonradiative energy loss through O—H vibration of H₂O molecule in Tb³⁺ complex. Under the optimum conditions, BSA can enhance the fluorescence intensity of the lomefloxacin-terbium complex at 545 nm, and the enhanced fluorescence intensity of Tb³⁺ is proportional to the concentration of BSA. Based on that, a new method is constructed for the determination of BSA. The linear ranges for BSA are 16.5-148.5 μg·mL-1 with a detection limit of 68.8 ng·mL^-1 and relative standard deviations of 1.4%. This method is simple, practical and relatively free of interference from coexistent substances.
		2006 Vol. 26 (03): 480-483 [Abstract] ( 1278 ) PDF (463 KB) ( 408 )

	484	Spectroscopic Characterization of Structure of Cerium Incorporated MCM-48 Mesoporous Molecular Sieve
		JIN Zhong-xiu, YONG Guo-ping, SHENG Liang-quan, TONG Hong-wu, SU Qing-de, LIU Shao-min^*
		Cerium incorporated MCM-48 molecular sieves have been hydrothermally synthesized using a mixed template and pH adjusting route. The samples were characterized by various physicochemical methods, including X-ray diffraction, diffuse reflectance UV-Vis spectroscopy, FTIR spectroscopy, XRF spectroscopy, and nitrogen adsorption. These results reveal that cerium is incorporated in MCM-48 and it is in the form of well-dispersed tetra-coodinated cerium ion. The proper concentration of cerium and adjusting pH can keep Ce-MCM-48 with higher specific surface area , larger unit-cell parameter, narrower pore-size distribution, and thicker pore wall than those of MCM-48. These deduce higher activity, and better selectivity, thermal stability and hydrothermal stability than those of MCM-48. The concentration of Ce incorporated in MCM-48 affects the vibration of the framewoke Si of molecular sieves.
		2006 Vol. 26 (03): 484-487 [Abstract] ( 1198 ) PDF (587 KB) ( 405 )

	488	Determination of Tetracyclines by a New Spectrum Technique
		GAO Hong, ZHAO Yi-bing, GUO Xiang-qun^*
		Tetracyclines are light-fluorescence substances, which cannot be detected directly by fluorometry. Herein a new spectrum method was proposed to detect tetracyclines directly by fluorometry. Under optimal conditions, the calibration graph is linear over the range 0.10-9.00 μg·mL^-1 for tetracyclines, and the detection limits of tetracycline, oxytetracycline, chlortetracycline and doxycycline are 0.065, 0.067, 0.068 and 0.070 μg·mL^-1,respectively.
		2006 Vol. 26 (03): 488-490 [Abstract] ( 1482 ) PDF (373 KB) ( 406 )

	491	Spectroscopic Properties of Salicylaldehyde Anil Zinc and Its Thin Film
		HAO Yu-ying¹,GAO Zhi-xiang¹,WANG Hua²,ZHOU He-feng²,MA Guo-zhang³,LIU Xu-guang²,XU Bing-she²
		A new light emitting material, salicylaldehyde anil zinc (SAZ), was synthesized. It can form high quality nano-scale amorphous thin films on clean glass substrates by vacuum evaporation. Its structure, crystallization, thermal stability, and optical property were investigated by IR spectra, DTA-TG analysis, XRD spectra, UV-Vis spectra, and fluorescence spectra. Its energy band structure was confirmed by cyclic voltammogram and optical absorption band edge. Results show that the SAZ film is a thermally stable material, and can emit intense green fluorescence with a peak wavelength at 508 nm and a full width at halfmaximum of 90.2 nm under UV irradiation. Its HOMO energy level is about -5.659 eV，LUMO energy level is about -3.054 eV，optical gap band is about 2.604 eV. The fluorescence decay of stored films under ambient atmosphere is more rapid than that of 8-hydroxyquinoline aluminum films. However, the fluorescence decay of the films under UV irradiation is slower than that of 8-hydroxyquinoline aluminum films.
		2006 Vol. 26 (03): 491-495 [Abstract] ( 1509 ) PDF (614 KB) ( 417 )

	496	Synthesis and Spectral Characterization of a Novel Fluorescent Functional Monomer 2,4-Dimethyl-7-Acrylamine-1,8-Naphthyridine
		ZHOU Yan-mei^{1, 3},XU Wen-guo¹,TONG Ai-jun²,TONG Chun-yuan²
		A new fluorescent functional monomer DMAAN has been designed and synthesized for the first time. The monomer was expected to bind with cytosine via complementary hydrogen bonding under certain experimental conditions. The synthetic procedure of DMAAN is as follows. A suspension of 2,6-diaminopyridine in phosphoric acid was added to acetylacetone. The mixture was warmed for thirty minutes on a steam-bath. Then 2,4-dimethyl-7-amino-1,8-naphthyridine(DMAN, mp 216-218 ℃) was obtained. DMAAN was prepared by the reaction of DMAN with acryloyl chloride in chloroform and triethylether mixed solvent. Acryloyl chloride in anhydrous chloroform was added dropwise over 30 minutes with constant stirring at 4 ℃. The melting point of the final product was 239-241 ℃. Mass spectrometry and proton NMR were used to confirm the chemical structure of DMAN and DMAAN. The products were also characterized by UV and fluorescence spectrometry.
		2006 Vol. 26 (03): 496-498 [Abstract] ( 1853 ) PDF (352 KB) ( 374 )

	499	Interaction Models of Cucurbit(n=6,7,8) Urils and Imidazo[4,5，f]1,10-Phenanthroline Derivates
		WANG Qiu-li¹,XUE Sai-feng^1*,MU Lan¹,ZHU Qian-jiang¹,TAO Zhu¹,ZHANG Jian-xin²
		The interaction structures and optical properties of host-guest of cucurbit[n=6-8]urils with synthesized three imidazo-[4,5,f]1,10-phenathroline derivates were investigated by using ¹H NMR technique, fluorescence spectrophotometry, and ultraviolet spectrophotometry. The experimental results reveal that cucurbit [6] uril (Q[6]) can only interact with guest salt of 2-(4-methyl)-phenyl-imidazo-[4,5,f]1,10-phenathroline (W1) with a ratio of 1∶1; however, cucurbit [7] uril (Q[7])can interact with all three guest salts of 2-(4-methylphenyl)-imidazo-[4,5,f]1,10-phenathroline(W1), 2-(2-methylphenyl)-imidazo-[4,5,f]1,10-phenathroline(W2)，and 2-(3-methyloxyl phenyl)-imidazo-[4,5,f]1,10-phenathroline(W3) with the same ratio of 1∶1; cucurbit [8] uril (Q[8]), except interaction with W1 with a ratio of 1∶2, can also interact with W2 and W3 with a ratio of 1∶1.
		2006 Vol. 26 (03): 499-504 [Abstract] ( 1118 ) PDF (653 KB) ( 409 )

	505	Study on Interaction of Caffeine and Theophylline with Bovine Serum Albumins
		BIAN Wei¹,WEI Yan-li²,WANG Ya-ping²,DONG Chuan²
		The interation of caffeine and theophylline with bovine serum albumins has been studied by fluorescence spectroscopy. The results indicated that enoxacin could bind with BSA strongly at molar ratio 1∶1 and the equilibrium constants were K_c=1.673×10⁴ L·mol^-1 and K_t=6.802×10³ L·mol^-1,respectively. Good linear Stern-Volmer lines were observed on the fluorescence of BSA quenched by enoxacin of different concentration, indicating that the combination reaction of enoxacin with BSA is a single static quenching process.
		2006 Vol. 26 (03): 505-508 [Abstract] ( 1573 ) PDF (437 KB) ( 416 )

	509	Reaction of Atrazine with Protein and Its Application to Detection of Protein
		SHI Deng-rong^{1, 2, 3},ZHANG Tao¹,REN Li-ping^1*,YU Jin-lian³,ZHANG Chun-rong¹,RAO Zhen-hong¹
		The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin(BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O—H…π hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 μg·mL^-1. Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3σ) is 12 ng·mL^-1. Synthesis samples were determined with satisfactory results.
		2006 Vol. 26 (03): 509-512 [Abstract] ( 1735 ) PDF (513 KB) ( 413 )

	513	Synthesis and UV/Vis Spectra of a Dialkyloxy Substituted Phthalocyanine
		WU Shao-feng, XU Xiu-zhi, LIN Mei-jin, WANG Jun-dong^*,CHEN Nai-sheng, HUANG Jin-ling
		A dialkyloxy substituted metal-free phthalocyanine was synthesized by using 3-(2’,2’,4’-trimethyl-3’-pentoxy)-1,3- diiminoisoindoles and 1,3,3-trichloroisoindolenine as starting materials, and characterized by element analysis, ¹H NMR, IR and UV/Vis spectra. The dependences of wavelength of UV/Vis spectra and its split of Q band absorption on the number of substituents and, dielectric constant of solvents are discussed based on the comparison with tetraalkyloxy substituted metal-free phthalocyanine
		2006 Vol. 26 (03): 513-516 [Abstract] ( 1536 ) PDF (487 KB) ( 407 )

	517	Study on the Characterizations of β-Cyclodextrin-Benzoylferrocene- Thiosemicarbazone Inclusion Complex and Micro-Environmental Effects
		ZHANG Jing, LIU Wan-yi^*,ZHANG Xia
		Inclusion complex of benzoylferrocene-thiosemicarbazone(TBF) with β-cyclodextrin(β-CD) was prepared by using kneading method. Continuous variation plot proved that 2∶3(TBF∶β-CD) inclusion complex has been formed. The characterizations of the inclusion complex were studied by UV, FTIR and X-ray diffractometry. By using the data of UV spectroscopy, the binding constant of the inclusion compound was estimated to be 5.04×10¹³(L⁴·(mol)^-4), and its change in absorption with time was studied. The stability of inclusion is obviously greater than the guest molecule. Furthermore, the influences of different solvents and pH on the UV spectra of TBF and β-CD-TBF were discussed respectively. The results show that different micro-environments have apparently different effects on electric spectra of guest and inclusion complexes.
		2006 Vol. 26 (03): 517-521 [Abstract] ( 1082 ) PDF (609 KB) ( 411 )

	522	Separation and Determination of Selenium Polysaccharide in Enriched-Se Cordyceps Militaris
		DENG Gui-chun¹,HOU Song-mei¹,TIAN Dong-mei²,ZHANG Yu-yang¹,ZHANG Xin¹,GU Jia-li¹,ZANG Shu-liang^1*
		In the present paper, the water soluble selenium polysaccharide was extracted, and isolated and purified preliminarily. The traditional method was used to extract selenium polysaccharide, which was extracted by water and sank by ethanol. The work also focused on the determination of the experiment conditions, optimizing the experiment conditions such as proportion of extract solution, extract temperature, extract times, proportion of ethanol, and standing time. The recovery of selenium polysaccharide under these conditions is 5.76%. Polysaccharide content in the powder is 46.6%, and selenium content is 92.3 mg·kg^-1.
		2006 Vol. 26 (03): 522-525 [Abstract] ( 1595 ) PDF (522 KB) ( 383 )

	526	Comparative Study on GOD-Trinder Method for Measuring Glucose in Serum with Different Benzene-Original Phenols
		XU Sheng¹,DENG Jian^1*,XU Jin-sheng²,YUAN Ya-li¹,ZHANG Wei¹
		Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H₂O₂) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P＞0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.
		2006 Vol. 26 (03): 526-528 [Abstract] ( 1574 ) PDF (359 KB) ( 422 )

	529	Study on Determining the Composition of Ternary Complex (Al-CAS-CTMAB) with the Ratio of Double Peak Values at Double Wavelengths by UV Spectrophotometry
		ZHANG Lin-lin, ZHENG Wen-zhi, LIN Jin-wei, ZENG Wei
		In the present paper，the composition of complex Al-CAS-CTMAB was studied with the ratio of double peak values at dual-wavelengths by UV spectrophotometry. First, the composition of complex of Al-CAS was determined in this method，Al∶CAS=1∶2. The absorption of ternary complex(Al-CAS-CTMAB) was determined with reference reagent of the complex Al-CAS. The ε values of Al-CAS and Al-CAS-CTMAB were determined with water reference. The composition of complex Al(CAS)n₁(CTMAB)n₂ was calculated with the formula: N=n₁/n₂=(ΔA₂ε^MR_λ₁-ΔA₁ε^MR_λ₂)/(ΔA₂ε^R_λ₁-ΔA₁ε^R_λ₂) CAS∶CTMAB=1∶1;Al∶CAS∶CTMAB=1∶2∶2. The calculated value and that obtained by traditional method are agreeable. The method is reliable for determining composition of ternary complexes.
		2006 Vol. 26 (03): 529-531 [Abstract] ( 1100 ) PDF (276 KB) ( 377 )

	532	Determination of Several Substrate-Decomposition Enzyme Activities of Agrocybe Cylindracea at Different Stages of Development with Spectrophotometer
		FU Ming-hui, KONG Min, HAN Ya-li
		In the present paper the authors studied the activities of amylase, peroxidase, laccase, protease and cellulase at different developmental stages of agrocybe cylindracea and its mutation with the spectrophotometer. The result showed that the activity of amylase and peroxidase in hyphal stage is the highest for the two basidiomycetes. It can be concluded that these two enzymes are important in vegetative growth stage, but they may have little effect after the appearance of fruit body. The change of their protease is similar: in the hyphal growth stage it is higher than that of mature mushroom. But the activities of laccase and cellulase are very different between the two basidiomycetes. It is obvious that they are some different in physiology and biochemistry.
		2006 Vol. 26 (03): 532-534 [Abstract] ( 1574 ) PDF (357 KB) ( 371 )

	535	Simultaneous Determination of Copper(Ⅱ) and Chromium(Ⅲ) by Absorbance Ratio-Derivative Method
		ZHANG Shu-fang
		In the present paper, an absorbance ratio-derivative method for the simultaneous determination of copper(Ⅱ) and chromium(Ⅲ) was proposed. In the HAc-NaAc buffer solution at pH 5.7 with heating, Cr³⁺ or Cu²⁺ with CAS and CTMAB formed a blue ternary complex. The molar absorptivities of Cr(Ⅲ)-CAS-CTMAB and Cu(Ⅱ)-CAS-CTMAB were 2.52×10⁵ L·mol^-1·cm^-1 and 1.01×10⁵ L·mol^-1·cm^-1 respectively. Beer’s law was obeyed in the range of 0.08-1.2 μg·mL^-1 for Cu(Ⅱ) and 0.05-0.52 μg·mL^-1 for Cr(Ⅲ). The detection limits of Cu(Ⅱ) and Cr(Ⅲ) are 0.014 μg·mL^-1and 0.013 μg·mL^-1 respectively. This method was applied to the determination of Cu(Ⅱ) and Cr(Ⅲ) in surrounding water with satisfactory results.
		2006 Vol. 26 (03): 535-538 [Abstract] ( 1440 ) PDF (423 KB) ( 383 )

	539	Study on Photoelectric Dispersion Characteristic of Liquid Crystal Light Valve
		HUANG Chong¹,ZHOU Xue-ping¹,OUYANG Yan-dong¹,LIN Xu-sheng¹,WU Yong-jun²,HUANG Yuan-ming¹
		The electro-optical properties and dispersion characteristics of a liquid crystal light valve (LCLV) were investigated. A 90° twisted light valve (model TB3639) was used as a typical LCLV in the authors’ experiment. At 27 ℃ and a fixed frequency of 1 000 Hz, the transmitted light intensities (T) of the LCLV were measured as a function of wavelength (λ) at different applied voltages. From the measured T-λ curves, the dependence of contrast ratio k on the wavelength can be derived. The authors’ results showd that the TB3639 LCLV has a comparatively high contrast ratio and low dispersion within the visible region. In the range of 450-750 nm, the contrast ratio, with its value higher than 0.8, does not change much with the wavelength. Particularly the contrast ratio is higher than 0.95 with a minimum dispersion in the range of 550-670 nm.
		2006 Vol. 26 (03): 539-541 [Abstract] ( 1616 ) PDF (451 KB) ( 390 )

	542	Research Advance in Slurry Sample Introduction for Plasma Emission Spectrometry/Mass Spectrometry
		WANG Zheng^{1, 2},QIU De-ren²,TAO Guang-yi¹,YANG Peng-yuan^2*
		The present review is focused on the advances and applications of slurry introduction for plasma spectrometry/mass spectrometry. Preparation and characterization of the suspensions are described, including the methods for decreasing the particle size such as the bottle and bead method, mixing mill method, vibration mill method, and supersonic mill method. Dispersion of the particles can be controlled via adding dispersant and pH adjustment. Some methods for particle size measurement, namely, sedimentation, optical microscopy, photosedimentometry, laser diffraction, scanning electron microscopy and transmission electron microscopy, are also described. Effects of suspension concentration and calibration techniques, including calibration by simple aqueous standard, internal standard, correction by empirical correction factors, standard additions, intrinsic internal standardization, and standard slurries, are discussed. Fundamental study of slurry introduction and its applications to plasma spectrometry/mass spectrometry are reviewed.
		2006 Vol. 26 (03): 542-547 [Abstract] ( 1604 ) PDF (674 KB) ( 396 )

	548	Determination of Eleven Elements in Water-Touched Material by ICP-MS
		Lü Lin-lin¹,XU Ye^1*,YI Ping²,QU Ning²,GU Xin-rong¹
		Water-touched material include water distribution equipment, protecting materials, and water treatment material. The security of the material plays an important role in everyone’s daily life. So it’s necessary to determing metal elements in the material. A method for the determination of eleven elements, including Ag, As, Ba, Cd, Cu, Sb, Sn, Mn, Ni, Pb and Hg, in the water-touched material by inductively coupled plasma-mass spectrum(ICP-MS) is described. The plasma parameters were optimized as follows: The RF input power was 1 350 W; flow rate of cooling gas was 13 L·min^-1; flow rate of assistant gas was 0.70 L·min^-1; flow rate of carrying gas was 0.8 L·min^-1; flow rate of atomization gas was 1.02 L·min^-1; rate of sampling was 1.48 mL·min^-1. The detection limits of these eleven elements were 0.003-0.170 μg·L^-1. The relative standard deviations(n=6) were 0.6%-6.3%. The correlation coefficients were 0.999 0-0.999 6. The recoveries of the method were 92.4%-108.2%. This method is sensitive, accurate and simple compared with other methods, and has the advantage of wide linear range. The results were satisfactory.
		2006 Vol. 26 (03): 548-550 [Abstract] ( 1633 ) PDF (305 KB) ( 383 )

	551	Study on the Method of Using ICP-MS to Determine Se in the Edible Fungi
		TIE Mei^{1, 2},ZANG Shu-liang^{1, 2*},ZHANG Wei²,LI Jing²,SUN Tie-biao²,LI Hua-wei³
		The sample was digested by the quartzose digestion pot at high-pressure under relatively low temperature, reducing the loss of Se during the digestion process. Study on the method of using ICP-MS to determine Se in the edible fungi shows that it exhibits positive correlation between the concentration of sample and integration time, and the response value. When the concentration of Se in the sample is higher than 50 μg·L^-1,the integration time can be 0.1 s; or it can be 2.0 s when the concentration of Se is lower than 5 μg·L^-1. The standard curve was accomplished for 1.0-500 μg·L^-1 when determining the content of Se in the edible fungi. The recoveries are in the range of 99.96% -102.7%. This method has advantages of simplicity, speediness, delicacy, high stability, high accuracy, etc. It is suitable for the determination of trace Se in the edible fungi.
		2006 Vol. 26 (03): 551-553 [Abstract] ( 1650 ) PDF (425 KB) ( 411 )

	554	Determination of Lead and Arsenic Contents in Amylum by ICP-AES Methodology
		ZHANG Yi-min,JIANG Hui,Lü Xue-bin,WANG Hong
		Lead and arsenic contents in amylum were determined by ICP-AES (inductively coupled plasma atomic emission spectroscopy) method with the addition of Ni(NO₃)₂ as the matrix modifier. Detailed discussion was conducted about the impact of factors such as ashing and automation temperature, ashing method of samples, and matrix modifier on the experimental results. The experimental results showed that ICP-AES method is easy, rapid and exact, its return character is good, and it can be used in the inspection of heavy metals such as lead and arsenic in amylum.
		2006 Vol. 26 (03): 554-556 [Abstract] ( 1116 ) PDF (312 KB) ( 373 )

	557	Simultaneous Determination of Heavy Metal Elements in Footwear Materials by ICP-AES
		Lü Shui-yuan, LIU Wei, LIN Hua, SUN Hua-hua
		Footwear materials were treated by the digestion method of carbonization-crack-HNO₃-HClO₄ dissolution, and the heavy metal elements in these materials were simultaneously determined in the same treated solution. Factors such as the selection of analytical wavelength and the interference of matrix and coexistent elements were studied. The method was secure, convenient, rapid and accurate. The recovery rates of the procedure were between 92.0% and 102.0% with RSDs between 1.0% and 4.6%.
		2006 Vol. 26 (03): 557-560 [Abstract] ( 1577 ) PDF (451 KB) ( 389 )

	561	Pretreatment of Lead-Antimony Alloy and Determination of Antimony and Selenium Using ICP-AES
		FANG Yi-wen, SONG Yi-bing, SUN Chang-yong, XUE Liang, YU Lin
		The sample was obtained from original sample using “sawing method” and was dissolved with a mixed solvent of nitric acid and tartaric acid. After the sample solution was pretreated with a series of methods, Sb and Se were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The results show that the recovery of standard addition of Sb was 96.2% and that of Se was 92.1%, and that the relative standard deviations(n=11)were smaller than 3.56%. The method is efficient, accurate and easy to operate, and has been applied to the determination of Sb and Se in Pb-Sb alloy products with satisfactory results.
		2006 Vol. 26 (03): 561-563 [Abstract] ( 578 ) PDF (362 KB) ( 409 )

	564	Application of Internal Standardization to Rapid Coprecipitation Technique Using APDC-Cu(Ⅱ) for FAAS Determination of Lead in Salt
		SU Yao-dong, LI Jing, HUANG Yan, CHEN Long-wu
		A method was proposed for the determination of trace lead in salt with flame atomic absorption spectrometry after preconcentration of lead by rapid coprecipitation technique with APDC-Cu(Ⅱ) using nickel as an internal standard at pH 2.5. The standard addition recovery of lead is between 92%-101%. The detection limit is 3.27×10^-3 μg·mL^-1 when the sample volume is 100 mL. The effect of matrix can be overcome by the method，and the results are satisfying. The method proposed here is rapid and has good reproducibility.
		2006 Vol. 26 (03): 564-566 [Abstract] ( 1990 ) PDF (376 KB) ( 400 )

	567	Determination of Trace Elements in Pumpkin by FAAS
		FAN Wen-xiu¹,LI Xin-zheng²,JING Rui-jun¹
		Conditions for simultaneous determination of multi-trace elements in pumpkin by FAAS were studied. K, Ca, Mg, Na, Zn, Cu, Mn, Fe, Co and Cr in different parts and different growth periods of pumpkin were determined, the relative standard deviation of the method is between 0.1% and 2.6%, and the recovery is between 96.7% and 106.5%. Analytical results were satisfactory. The results showed that some elements essential to human such as K, Ca, Mg, Zn, Cr and Mn in pumpkin are abundant, implying that the nutritive value of pumpkin is high.
		2006 Vol. 26 (03): 567-570 [Abstract] ( 1617 ) PDF (551 KB) ( 432 )

	571	X-Ray Absorption Spectroscopy Study on Nanowires and Nanotubes of Carbon and Silicon
		LIN Liang-wu, TANG Yuan-hong^*,ZHANG Wei
		Due to the different sampling depth, the total electron yield (TEY) is sensitive to the surface and near surface region, while the fluorescence yield (FLY) probes the information of the bulk. Thus the combined use of TEY and FLY provides a powerful evidence for identifying the whole sample whether or not it is a nanoscale material, and is a supplement of the conventional methods for characterizing nanoscale materials, such as TEM and XRD. With analyses of X-ray absorption spectra recorded in TEY and FLY mode, it could be used for studying the mechanism of growth, orientation, chemical bonding, defect and helicity of nanowires and nanotubes exactly and reliably. Therefore, it is believed that X-ray absorption spectroscopy is a powerful characterization tool for the study of nanoscale materials, which has some super advantages over conventional methods.
		2006 Vol. 26 (03): 571-576 [Abstract] ( 1636 ) PDF (802 KB) ( 407 )

	577	The Application of Several Types of Large-Scale Exactitude Equipments in the Identification of Jadeite
		LIU Yan^{1, 2},DENG Jun^1，2,WANG Li-hua¹
		Infrared spectrometer，Raman spectrometer，EPMA, and X-ray powder diffractometer are very effective equipments for the identification of jadeite. Some kinds of raw jadeite fakes occurred in the identification and jewelry market recently. With the methods of infrared spectrometer，Raman spectrophotometer，EPMA, and X-ray powder diffractometer etc., these typical fakes have been systematically studied. These fakes have superficial resemblances such as texture，outer characters and color of minerals in rocks. The relevant information of these fakes has been obtained. Meanwhile, several kinds of rough materials with fake weathering crust and cutting groove were analyzed. These identification methods were compared with each other. Type-B can be tested by infrared spectrometer，Raman spectrophotometer, and X-ray powder diffractometer. The present paper means a lot for the identification, evaluation and business of jadeite.
		2006 Vol. 26 (03): 577-582 [Abstract] ( 558 ) PDF (932 KB) ( 400 )

	583	An Auto-Extraction Method of Spectral Line for Normal Galaxy Spectra
		LIU Rong¹,LIU San-yang¹,ZHAO Rui-zhen²
		Spectral line extraction for normal galaxy spectra is the most difficult task in spectral line auto-extraction of celestial spectra. The present paper presents a novel technique of spectral line auto-extraction for normal galaxy spectra. Firstly, the Max operator of two spectra is defined, and the operator produces a new spectrum whose intensity at each wavelength is the maximum of intensities of the two spectra; Secondly, the continuum is fitted in an iterative way, where in each iteration, the traditional wavelet method is performed for the spectrum obtained by the Max operation of the original spectrum, and the continuum is fitted in the last iteration; Finally, adaptive local thresholding associated with the universal thresholding is used to extract spectral lines. Experiments show that this method is superior to the traditional wavelet method. It will be helpful to the spectral line based automated spectral classification and parameter measurement.
		2006 Vol. 26 (03): 583-586 [Abstract] ( 1133 ) PDF (523 KB) ( 391 )

	587	A Novel Method for Continuum Normalization of Astronomical Spectrum Signals
		ZHAO Rui-zhen¹,LUO A-li²
		A combined method of wavelet transform and spline fitting is presented for continuum fitting with the strong spectral lines taken out beforehand. Some comparisons between normal methods and the method presented are made by the experiments on actual spectra. And the experimental results show that our method is superior to others. Besides, an effective continuum revision formula is derived to avoid the appearance of negative continua obtained by some methods such as polynomial fitting and so on.
		2006 Vol. 26 (03): 587-590 [Abstract] ( 1093 ) PDF (455 KB) ( 515 )

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