| 光谱学与光谱分析 |
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| Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method |
| GUO Yu-hua1,CHEN Xiao-lan1,ZHANG Ting1,QU Ling-bo1, 2*,ZHAO Yu-fen1, 3,YU You-zhu1 |
1. Key Laboratory for Bio-Chemistry and Organic Chemistry, Zhengzhou University, Zhengzhou 450052, China
2. Anyang Teachers College, Anyang 455002, China
3. Key Laboratory for Bio-organic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China |
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Abstract The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide(EB)as a probe. The result showed that both 7-hydroxy flavone and its phosphate could form non-covalent complexes, but the phosphorylated flavonoid showed higher binding affinity with DNA than 7-hydroflavone did. Experiments demonstrated that the higher the temperature, the lower the slop of quenching curve of DNA-EB in the presence of different amounts of 7-hydroxy flavone and its phosphate. It was confirmed that the combinations of DNA with 7-hydroxy flavone and its phosphate were a single static quenching process. According to the Stern-Volmer equation and Scatchard equation the quenching constants and the intrinsic binding constants of 7-hydroxy flavone and its phosphate were measured respectively, they were Kq1= 601 L·mol-1,Kq2=1 381 L·mol-1;K1=2.07×104 L·mol-1,K2=3.19×104 L·mol-1 respectively.
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Received: 2004-12-26
Accepted: 2005-05-11
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Corresponding Authors:
QU Ling-bo
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| Cite this article: |
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GUO Yu-hua,CHEN Xiao-lan,ZHANG Ting, et al. Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 475-479.
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https://www.gpxygpfx.com/EN/Y2006/V26/I03/475 |
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