| 光谱学与光谱分析 |
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| Comparative Study on GOD-Trinder Method for Measuring Glucose in Serum with Different Benzene-Original Phenols |
| XU Sheng1,DENG Jian1*,XU Jin-sheng2,YUAN Ya-li1,ZHANG Wei1 |
1. College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China
2. Hengyang Normal College, Hengyang 421008, China |
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Abstract Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H2O2) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P＞0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.
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Received: 2005-01-06
Accepted: 2005-04-10
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Corresponding Authors:
DENG Jian
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| Cite this article: |
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XU Sheng,DENG Jian,XU Jin-sheng, et al. Comparative Study on GOD-Trinder Method for Measuring Glucose in Serum with Different Benzene-Original Phenols[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 526-528.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I03/526 |
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