| 光谱学与光谱分析 |
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| Reaction of Atrazine with Protein and Its Application to Detection of Protein |
| SHI Deng-rong1, 2, 3,ZHANG Tao1,REN Li-ping1*,YU Jin-lian3,ZHANG Chun-rong1,RAO Zhen-hong1 |
1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China
2. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
3. Department of Environmental Science and Engineering, College of Tourism, Shanghai Normal University, Shanghai 200234, China |
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Abstract The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin(BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O—H…π hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 μg·mL-1. Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3σ) is 12 ng·mL-1. Synthesis samples were determined with satisfactory results.
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Received: 2005-10-06
Accepted: 2005-12-28
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Corresponding Authors:
REN Li-ping
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| Cite this article: |
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SHI Deng-rong,ZHANG Tao,REN Li-ping, et al. Reaction of Atrazine with Protein and Its Application to Detection of Protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(03): 509-512.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I03/509 |
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