光谱学与光谱分析 |
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Raman Spectroscopic Study on the Influence of Ultraviolet Radiation on Calf Thymus DNA in Aqueous Solution |
ZHOU Dian-feng,KE Wei-zhong* |
Jiangsu Provincial Key Lab for Optoelectric Technology, Nanjing Normal University,Nanjing 210097,China |
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Abstract The Raman spectra of calf thymus DNA in aqueous solution and at 9, 20 and 40 min after ultraviolet radiation were reported. The experimental results proved that though the ultraviolet radiation time was not long, it had a serious influence on the DNA. Only through 9 min of ultraviolet radiation, the peak of 1 094 cm-1 changed into a few weaker peaks,and it was proved that the molecular conformation was changed and the hydrogen bonds were damaged. Some kinds of nucleotides came into being because of the damage to the DNA molecular. Ultraviolet radiation also had influence on adenine, guanine, cytosine and thymine. Among them the purine and pyrimidine were badly damaged. At the same time, our experiment proved that DNA in aqueous solution possesses both A-type structure and B-type structure.
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Received: 2003-06-18
Accepted: 2003-10-29
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Corresponding Authors:
KE Wei-zhong
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Cite this article: |
ZHOU Dian-feng,KE Wei-zhong. Raman Spectroscopic Study on the Influence of Ultraviolet Radiation on Calf Thymus DNA in Aqueous Solution [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1370-1372.
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URL: |
http://www.gpxygpfx.com/EN/Y2004/V24/I11/1370 |
[1] SHEN Ren-quan, GU Qi-min(沈仁权, 顾其敏). A Course of Biochemistry(生物化学教程). Beijing: High Education Press(北京:高等教育出版社),1993. 434. [2] WANG Jie-fang, LI Fu-guang, GUO Mao-tian(王杰芳,李富广,郭冒田). Laser Journal(激光杂志),1999, 20(6):12. [3] Anthony T Tu. Raman Spectroseopy in Biology: Principles and Applications. New York: John Wiley and Sons, 1982. 145. [4] ZHAO Hong-xia, XU Yi-ming, ZHANG Zhi-yi(赵红霞,许以明,张志义). Chinese Science Bulletin(科学通报), 1998, 43(9):957. [5] KE Wei-zhong,YU Duo-wei, CHEN Wan-rong et al(柯惟中, 余多慰, 陈婉容等). Acta Optica Sinica(光学学报), 1997, 17(12):1681. [6] KE Wei-zhong, YU Duo-wei. Opt. Eng., 1994, 33(8): 5672.
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