| 光谱学与光谱分析 |
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| Temperature Dependence of Collagen by Raman Spectra |
| DONG Rui-xin1，2, YAN Xun-ling1, JIANG Shan2, LIU Mei-jie2, LIU Sheng-gang2 |
1. Physics Department of Liaocheng University, Liaocheng 252059，China
2. University of Electronic Science and Technology of China, Chengdu 610054，China |
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Abstract Raman spectra of collagen Ⅰ at different temperature were obtained. There was no change at 1 003 cm-1 line, 1 302 cm-1 line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 ℃ respectively. The points at 40 and 68 ℃ are in agreement with the experimental data by DSC and SHG. The point at 0 ℃ might be frozen transition; the point at 90 ℃ might be related to the damage of secondary structure. When heated to 150 ℃, the Raman intensity of all bands decreased rapidly and many lines disappeared.
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Received: 2003-05-27
Accepted: 2003-10-12
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Corresponding Authors:
DONG Rui-xin
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| Cite this article: |
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DONG Rui-xin,YAN Xun-ling,JIANG Shan, et al. Temperature Dependence of Collagen by Raman Spectra [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1367-1369.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I11/1367 |
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