| 光谱学与光谱分析 |
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| TLC-FT-SERS Study on a Pair of Optic Isomers in Ephedra |
| WANG Yuan1，ZHANG Jin-zhi2，MA Xin-yong1 |
1. Department of Chemistry, Capital Normal University, Beijing 100037，China
2. North University of Technology, Beijing 100041, China |
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Abstract A new method for analyzing the ingredients of a pair of optic isomers in ephedra, nor-ephedrine and nor-pseudoe-ephedrine, using hyphenated high-efficiency thin layer chromatography (TLC) and surface-enhanced Raman spectroscopy (SERS) techniques, is reported. The results show that the characteristic spectral bands of nor-ephedrine and nor-pseudo-ephedrine can be obtained from the TLC spot with 8 μg sample of about 2.0 mm in diameter. The difference between the SERS and solid spectra was found. Spectral bands at 1 004 cm-1 and 1 605 cm-1 were found greatly enhanced. Molecule was absorbed in surface silver sol by π electrons in ring. Under similar experimental conditions the spectral information of Levo-nor-ephedrine ramifications TLC-SERS is rich with strong credibility, whereas dextral-nor-ephedrine ramifications show a relatively strong fluorescence backdrop with less spectral information and weak credibility. The effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.
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Received: 2004-03-08
Accepted: 2004-06-26
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Corresponding Authors:
MA Xin-yong
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| Cite this article: |
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WANG Yuan,ZHANG Jin-zhi,MA Xin-yong. TLC-FT-SERS Study on a Pair of Optic Isomers in Ephedra [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1373-1375.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I11/1373 |
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