Abstract:The inclusion complexes of β-Cyclodextrin (β-CD) and HP-β-Cyclodextrin (HP-β-CD) with 6-Mercaptopurine (6-MP), Azathioprine (BAN) and 8-Azaguanine (Azan) were investigated by fluorescence. Various factors affecting the formation of inclusion complexes were discussed in detail including formation time and pH effect. The formation constants of their inclusion complexes were determined. The results indicated that their inclusion was affected significantly by laying time and pH. The formation time of β-CD inclusion complexes is much longer than that of HP-β-CD. The optimum pH is about pH=7.7-12. Their maximum excitation wavelengths are all in the range of 276-285 nm and the maximum emission wavelengths are all in the range of 328-353 nm. The fluorescence signals are intensified with increasing concentration of CD. The stoichiometries of the inclusion complexes of CD with these three anticancer xanthines are all 1∶1 and the formation constants are calculated.
卫艳丽,董 川* . 三种抗癌嘌呤类化合物与环糊精作用的研究 [J]. 光谱学与光谱分析, 2004, 24(07): 862-866.
WEI Yan-li, DONG Chuan* . A Study on Inclusion Complexes of Cyclodextrin with Three Anticancer Xanthines by Fluorescence . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 862-866.
[1] Nelson J A, Carpenter J W, Rose M L et al. Cancer Res., 1975, 35: 2872. [2] China Dictionary of Drugs(Ⅱ)(中华人民共和国药典(Ⅱ)). Beijing:Science Press(北京:科学出版社),1990. 758. [3] ZHU Sheng-shan(朱盛山). New Forms of Drugs. 1st Ed(药物新剂型(第一版)). Beijing:People′s Health Press(北京:人民健康出版社),1993. 132. [4] XIE Xu-yi(谢旭一). Modern Medicine Application of China(中国现代应用药学),1997,14(6):30. [5] ZHANG Yong(张 勇). Chinese Journal of Analytical Chemistry(分析化学),1998,26(6):729. [6] Tong Lin-hui Editor(童林荟编著). Cyclodextrin Chemistry——Foundation and Application(环糊精化学——基础与应用). Beijing:Science Press(北京:科学出版社),2001. 146.