Abstract：The absorbance, fluorescence and Scatchard plots methods as well as the effect of phosphate group on the fluorescence intensity of the cordycepin-DNA-EB system were used to study the interaction of the antitumor compound cordycepin and DNA. It is obvious tiat there is hyperchromic effect and hypochromic effect with slight red shift on the subtracted UV spectrum. It proves that the adenine base of cordycepin can be inserted into the double-helix of DNA. The results of the fluorescence intensity that decreases after increases with a little blue shift on the fluorescence spectrum also proved this. At the same time the phosphate can affect the cordycepin-DNA-EB system. Finally that the result depended on the Scatchard equation indicates that cordycepin reacts electrostatically on phosphate backbone of DNA. There also exists an intercalation into the double-helix of DNA.
彭俊峰，凌建亚，张晗星，张长铠* . 虫草素与DNA作用的光谱研究 [J]. 光谱学与光谱分析, 2004, 24(07): 858-861.
PENG Jun-feng，LING Jian-ya，ZHANG Han-xing，ZHANG Chang-kai* . Study on the Interaction of Cordycepin and DNA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 858-861.
[1] Ling Jian-ya, Sun Ying-jie, Zhang Hua et al. J. Bioscience Bioengineering，2002, 94(4): 371. [2] Lee M, Ｋrowick Ｋ et al. J. Am. Chem. Soc.，1988, 110: 3641. [3] SHEN Tonｇ(沈 同编著). Biochemistrｙ(生物化学(上册)). Beijinｇ：People′s Edｕcation Press(北京：人民教育出版社)，1989. [4] Wu K C, Lippard S J. Biochemistry, 1976, 15(19): 4339. [5] Lepecq J B, Paoletti C. J. Mol. Biol., 1967, 27: 87. [6] GUO Mao-lin，YANG Pin，YANG Bin-shenｇ(郭茂林，杨 频，杨斌盛等). Science in China B(中国科学B辑)，1995，5. 517.
