Spectrofluorimetric Study of Supramolecular Inclusion between Rodenticide Brodifacoum and β-Cyclodextrin and Application
DUAN Yun-qing1, 2, DUAN Zhi-qing1, MIN Shun-geng2*, XIONG Yan-mei2，DUAN Jia2, YE Sheng-feng2, WANG Dong2
1. College of Art and Science, Shanxi Agricultural University, Taigu 030801, China 2. College of Science, China Agricultural University, Beijing 100193, China 3. Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118，China
Abstract：The supramolecular interaction between β-cyclodextrin and brodifacoum, an anticoagulant rodenticide of the second generation, was studied by spectroscopy. The results showed that brodifacoum and β-cyclodextrin could form an inclusion complex with an association constant of 1.048×104 L·mol-1 and a 1∶1 stoichiometry based on Benesi-Hildebrand equation. The inclusion mechanism was proposed to explain the inclusion mode. It was indicated that the hydrophobic group of brodifacoum molecule, biphenyl, entered into the cavity of β-cyclodextrin. At the same time, it was also observed the significant enhancement of fluorescence of brodifacoum after forming inclusion complex. According to the fluorescence enhancement phenomenon, a spectrofluorimetric method of detecting brodifacoum in aqueous media was established with the linear range of 8.0×10-8-4.0×10-6 mol·L-1 and the correlation coefficient of 0.999 4. The detection limit of the method was 8.8×10-9 mol·L-1. The proposed method was successfully applied to determine the trace amount of brodifacoum in environment water and the recovery was in the range of 87.3% to 103.9%.
段云青1,2，段志青1，闵顺耕2*，熊艳梅2，段 佳2，叶升峰2，王 冬2 . 光谱法研究杀鼠剂溴鼠灵与环糊精超分子体系及分析应用[J]. 光谱学与光谱分析, 2010, 30(04): 1065-1069.
DUAN Yun-qing1, 2, DUAN Zhi-qing1, MIN Shun-geng2*, XIONG Yan-mei2，DUAN Jia2, YE Sheng-feng2, WANG Dong2 . Spectrofluorimetric Study of Supramolecular Inclusion between Rodenticide Brodifacoum and β-Cyclodextrin and Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(04): 1065-1069.
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