Abstract：In the present study, β-cyclodextrin(β-CD) was used as chiral selector to detect the proportion of chiral isomers of metalaxyl. The proportion of metalaxyl enantiomers can be detected by ultraviolet(UV)spectroscopy since the interaction between the R, S isomer of metalaxyl with β-CD is different. The quantitative models were established by partial least squares regression (PLS) and the robust of models was evaluated by independent validation samples. The determination coefficient R2 of calibration set in the quantitative model was 0.999 0. The standard error of calibration set (SEC) and the relative standard deviation (RSD) of the model was respectively 0.006 7 and 0.89%; The correlation coefficient r of estimated value and specified value of the 6 independent validation samples was 0.998 5. The standard error of prediction (SEP) and RSD was respectively 0.008 9 and 1.17%. This method is rapid and easy to operate in practical applications.
Key words：Metalaxyl;β-cyclodextrin;Partial least squares;Ultraviolet;Chiral analysis
李倩倩，朱业伟，熊艳梅，段 佳，吴丽君，李春子，闵顺耕* . 甲霜灵手性异构体比例的紫外光谱法快速测定 [J]. 光谱学与光谱分析, 2010, 30(12): 3395-3398.
LI Qian-qian, ZHU Ye-wei, XIONG Yan-mei, DUAN Jia, WU Li-jun, LI Chun-zi, MIN Shun-geng* . Fast Determination of the Proportion of Metalaxyl-M Enantiomers by UV Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(12): 3395-3398.
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