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2006 Vol. 26, No. 08
Published: 2006-08-26
 
       光谱学与光谱分析
1377 Quantum Efficiency of the 5D0 Level of Eu3+ at C2 Site in Cubic Nanocrystalline Y2O3
MENG Qing-yu1, 2, CHEN Bao-jiu1*, XU Wu1, 3, WANG Xiao-jun1, XIE Yi-hua1, LAI Hua-sheng1, 4, DI Wei-hua1, REN Guo-zhong1, ZHAO Xiao-xia1, YANG Yan-min1
In the present the authors are trying to work out how the quantum efficiency depends on the nanocrystalline size. Cubic nanocrystalline Y2O3:Eu3+ samples were prepared by chemical self-combustion. The bulk Y2O3:Eu3+ was obtained by annealing the nanocrystalline at 1 000 ℃ for 2 h. The emission spectra, XRD and fluorescence decay showed that the emission intensities are increased and fluorescence decay becomes slow with an increase in particle diameter of the samples. Two routes were used to estimate the quantum efficiency of the 5D0 level of Eu3+ at C2 site. The quantum efficiencies of 5D0 level of Eu3+ at C2 site in the samples depend on the nanocrystalline sizes. Finally, a detailed discussion about these two approaches for estimating the quantum efficiencies was made.
2006 Vol. 26 (08): 1377-1381 [Abstract] ( 1632 ) PDF (686 KB)  ( 426 )
1382 Spectroscopic Methodology for the Measurement of Tokamak Plasma
YAO Bo, BAO Cheng-yu*, HE Ye-xi
The states and the distributions of the plasma in the tokamak can be obtained by measuring the spectrum. Since there are many different objects to be measured, many different apparatuses are used to get a better result. Some methods for the measurement are listed in this article. Also the measuring range of each method and some results are listed. On the other hand, a new method was developed according to the conditions of the lab.
2006 Vol. 26 (08): 1382-1386 [Abstract] ( 1629 ) PDF (621 KB)  ( 375 )
1387 Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates
CHEN Yan-mei1, HUANG Jian-dong1*, LIU Feng-ran1, SUN Rui-qing1, Dennis K P Ng2
The interactions of the new photosensitizer {2,3,9,10,16,17,23,24-octakis[(3,5-dicarboxy)phenoxy]-phthalocyaninato}zinc(Ⅱ) [ZnPc(COOH)16] and serum albumins (BSA and HSA) were investigated by absorption and fluorescence spectroscopy. The binding constants were found to be 2.25-2.94×106 L·mol-1. The 1∶1 ZnPc(COOH)16-BSA and ZnPc(COOH)16-BSA conjugates were also prepared by incubating a mixture of ZnPc(COOH)16 and the corresponding serum albumin in 2∶1 mole ratio followed by separation by gel permeation chromatography. The Q band absorption and fluorescence emission of ZnPc(COOH)16 are slightly red-shifted in the conjugates, and the spectral features suggest that the phthalocyanine exists predominantly in monomeric form in the conjugates.
2006 Vol. 26 (08): 1387-1391 [Abstract] ( 1737 ) PDF (551 KB)  ( 440 )
1392 Temporal Coherent Control in Er3+-Doped Telluride Glass
LI Yu-qiong,SUN Zhen-rong*,ZHANG Shi-an,YANG Xi-hua,WANG Zu-geng
The authors achieved the temporal coherent control in an Er3+-doped telluride glass，one kind of disordered solids. The upconversion at 670 nm was modulated and the dephasing time was simulated as 300 fs. Moreover，the photon echo signal gave the dephasing time due to the phonon interaction. The agreement between the two data indicates that the interaction between the wavepacket and the phonon leads to the fast disappearance of wavepackets interference，which is helpful for the technique to be applied to the disordered solids.
2006 Vol. 26 (08): 1392-1395 [Abstract] ( 1531 ) PDF (456 KB)  ( 355 )
1396 Upconversion of Oxide Y2O3 Doped with (Yb3+,Er3+) Ions with 978 nm Excitation
LIU Huang-qing, WANG Ling-ling, LI Hong-jian
Two nanomaterials Y2O3: Er3+ and Y2O3:(Yb3+, Er3+) were prepared by combustion on the basis of reagents Y2O3, Yb2O3 and Er2O3. The corresponding host Y2O3:(Yb3+, Er3+) was obtained by increasing calcination temperature. Upconversion emission was obtained under 978 nm LD excitation. Comparing nanopowder oxide Y2O3:Er3+ with nanometer materials Y2O3:(Yb3+, Er3+) with respect to upconversion spectra, the upconversion emission of the former mainly was green upconversion and that of the latter mainly red light. However, the upconversion emission of nanopowder Y2O3:(Yb3+, Er3+) was different from that of its corresponding host, with main red upconversion emission for the former but main green upconversion emission on the latter.
2006 Vol. 26 (08): 1396-1399 [Abstract] ( 367 ) PDF (436 KB)  ( 469 )
1400 Photoluminescence Properties of A2SnO4(A=Ca, Sr, Ba):Eu Nanocrystalline
FU Xiao-yan1, NIU Shu-yun1*, ZHANG Hong-wu2, MIN Qing-wang1, XIN Qin2
Nanosized A2SnO4(A=Ca，Sr，Ba):1％Eu with diameters ranging from 50 to 100 nm were synthesized by complex sol-gel methods. The structures and spectral properties of A2SnO4(A=Ca，Sr，Ba):Eu were studied by using X-ray powder diffraction (XRD) and luminescence spectra. The experimental results show that Ca2SnO4:1%Eu exhibits bright red emission at about 615 nm under UV excitation, which makes it a possible good candidate for FED application. However Sr2SnO4:1%Eu and Ba2SnO4:1%Eu exhibit orange emission at about 590 nm because in these hosts Eu3+ ions occupy the symmetric sites.
2006 Vol. 26 (08): 1400-1402 [Abstract] ( 1146 ) PDF (358 KB)  ( 393 )
1403 Luminescence of Rare Earth Complex in Organic-Inorganic Heterostructure
ZHANG Fu-jun1, XU Zheng1*, HUANG Jin-zhao1, WANG Yong1, SUN Bo2, PEI Juan2
Luminescence of rare earth complex based on organic-inorganic heterostructure is reported. The structure of the device is ITO/PVK:Tb/inorganic material/Al, where inorganic material includes ZnS, ZnO or ZnSe. For this structure, the authors obtained the characteristic emission of Tb ion. A large part of driving voltage was dropped on the PVK layer because the dielectric constant of ZnS is 3 times bigger than that of PVK. The electric field strength in PVK layer was improved, so the velocity of hole in PVK was increased. The balance of injected charge was enhanced.
2006 Vol. 26 (08): 1403-1405 [Abstract] ( 318 ) PDF (404 KB)  ( 403 )
1406 Effects of PBD on the Electroluminescence (EL) of Tb Complex Doped PVK System
XU Deng-hui1, DENG Zhen-bo1*, XU Ying1, XIAO Jing1, LIANG Chun-jun1,WANG Rui-fen2
Electroluminescent (EL) properties of the terbium complex [Tb(m-MBA)3phen]2·2H2O doped PVK system were investigated. Two kinds of devices with the structures of ITO/PVK:Tb complex/PBD/LiF/Al and ITO/PVK:Tb complex:PBD/PBD/LiF/Al were fabricated. PBD emission appears in the EL spectra of ITO/PVK:Tb complex/PBD/LiF/Al in comparison with ITO/PVK:Tb complex:PBD/PBD/LiF/Al. The reason is that PBD acts as a hole blocking material in the emitting layer (PVK: Tb complex: PBD) and thus the combination of excitons mostly occur within this layer. The energy of terbium complex emission comes from two different ways, namely energy transfer and direct combination of carriers. By altering the doping weight ratio of PBD, a set of devices were fabricated with the configuration of ITO/PVK:Tb complex:PBD/PBD/LiF/Al. Both the efficiency and brightness of these devices decrease with the rising of the PBD doping weight ratios. The efficiency of the carriers trapping may decrease with the rising of PBD doping ratios, for PBD may block the hoping of hole or electron between the PVK chains, causing a decrease in the brightness and efficiency. The PBD doping ratios have little effect on the efficiency of Frester energy transfer based on our synthesis.
2006 Vol. 26 (08): 1406-1410 [Abstract] ( 1400 ) PDF (508 KB)  ( 388 )
1411 Analyzing the Methods to Smooth Field Reconstructed by Algebraic Reconstruction Technique with Spectroscopy
SONG Yi-zhong1, 2,SUN Tao2,HU Guo-ying3,HE An-zhi1
The effects of filters and filtering methods on the field reconstructed by algebraic reconstruction technique (ART) is discussed with spectroscopy. The field to be constructed was simulated with numerical simulation technique. The effects of many kinds of filters and filtering methods on the field reconstructed by ART were analyzed with spectroscopy. The filters used included quarter, fifth, eighth averaging filter, fifth center filter, and third row, column filter. The filtering methods included filter being inserted in the iterating process or set at its end. The averaging filter mentioned above meant that the value was acquired from the average of the four data, named quarter averaging filter, and so on. The fifth center filter meant that the value was acquired from the center, the third one in ascendant or descendant sequence of the five data. As a result, the authors found that a larger difference existed in the effects of different filters and filtering methods on the reconstructed field. First, the field reconstructed by ART contained much big noise and needed to be smoothed. Secondly, the reconstructive accuracy declined much when an averaging filter, such as quarter, fifth or eighth filter, was only set at the end of iterating process. Thirdly, the accuracy could be improved a little with a quarter or eighth averaging filter inserted in the iterating process and activated according to iterating times. Forthly, a better accuracy could be obtained with a fifth center filter inserted in the iterating process than with a fifth averaging one inserted. Fifthly, the best result in this work was obtained with a fifth averaging filter and a fifth center one inserted in the iterating process and activated alternately according to iterating times.
2006 Vol. 26 (08): 1411-1415 [Abstract] ( 1056 ) PDF (647 KB)  ( 455 )
1416 Application of Fiducial Wavelength Method in Processing Spectra of Turbid Media
LUO Yun-han, GU Xiao-yu, XU Ke-xin*
In the present paper, the authors analyzed the absorption and scattering effects of changing the solution concentration on the spectra, and dealed with the wavelength, where the absorption and scattering effects counteract completely, the fiducial wavelength method can eliminate the perturbation from shift of instrument and temperature, and also can improve predicting ability of the model. The result of utilizing this method to process the spectral data of two sample sets, i.e. intralipid solution and plasma solution with different level glucose, has verified that fiducial wavelength method can improve correlation coefficient and decrease RMSEP of predicting model.
2006 Vol. 26 (08): 1416-1418 [Abstract] ( 1010 ) PDF (304 KB)  ( 368 )
1419 Analysis of LCD Aging with Polarized Optical Texture and Transmission Spectrum
HUANG Chong, ZHOU Xue-ping, LIU Ji, OUYANG Yan-dong
Light radiation experiment was done to a group of liquid crystal display devices, and the influence of light radiation on the display quality of liquid crystal display devices was studied. The changes in their polarized textures after light radiation were observed with a polarized optical microscope. Parallel stripe texture and black holes, which lost extinction function, appeared in liquid crystal texture. With the increase of light radiation time, more and more black holes result, and the area of black holes gets bigger and bigger. The authors tested the transmission spectrum with computer-controlled ultraviolet/visible light spectrometer, finding that the transmission rate declines with the increase of light radiation time. The results show that the appearance of stripe texture and black holes resulted from the changes in liquid crystal molecular structure after ultraviolet radiation, and the occurrence of black holes, which lost extinction function, is the main reason for the continuous decline of transmission rate.
2006 Vol. 26 (08): 1419-1422 [Abstract] ( 1124 ) PDF (796 KB)  ( 429 )
1423 Study on Remote Sensing of Methane Leakage Using a Tunable Diode Laser
FAN Hong1, 2, GAO Xiao-ming1, BAO Jian1, WANG Xia1, HUANG Teng1, HUANG Wei1, CAO Zhen-song1, ZHANG Wei-jun1
The leak of natural gas is not only an economic loss, but also the fountain of danger. Conventional detection techniques of natural gas pipe leak have low efficiency and slow respond time, therefore, it is difficult for them to suit practice application. Optical sensors based on NIR tunable diode laser absorption spectroscopy were widely used because of high sensitivity, small volume and less maintenance. In the present paper, a portable remote sensor of natural gas pipeline leak was reported. The sensor used a ratio of second to first harmonic signals as calibration method, and the results show a good consistency between the concentrations and the ratios of second to first harmonic signals. The effect of different topographic scattering targets on the ratio detection was measured and analyzed. The results show that the ratio of second to first harmonic signals can be used in practical application.
2006 Vol. 26 (08): 1423-1427 [Abstract] ( 1245 ) PDF (638 KB)  ( 480 )
1428 Study on Highly Effective Emulsified Viscosity Reducer FTIR and +H NMR
GUAN Run-ling,ZHU Hong*
Separation and purification of a highly effective emulsified viscosity reducer were performed. The functional group of the components of the sample was characterized by FTIR. The corresponding components were indentified with +H NMR and ESI-MS. It was deduced that the surfactants in the sample are 26.6% alkylphenol polyoxyethylene and 19.3% sodium lauroyl sarcosine. The macromolecule component in the sample is polybutylacrylate accounting for 9.0%. There is 27.2% NaOH in the sample and a little NaCl by qualitative and quantative analysis, and the residual is water.
2006 Vol. 26 (08): 1428-1431 [Abstract] ( 1617 ) PDF (352 KB)  ( 411 )
1432 Processing GC-FTIR by the Blind Source Separation
YAO Zhi-xiang1, 3, HUANG Hong2, LIU Huan-bin1
An analysis method for separating chromatographic overlapped peaks and purifying infrared spectra is put forward, based on the blind source separation technique and the multi-dimensional data of GC-FTIR, Using various information from hyphenated instruments, this method was used to separate completely a organic mixture, the xylene isomerism system, a problem unable to solve usually. The method can confirm the rationality of theory and algorithm and give integral explanations of the independent component analysis data. The reason for the error in quantitative analysis is discussed.
2006 Vol. 26 (08): 1432-1436 [Abstract] ( 2059 ) PDF (561 KB)  ( 381 )
1437 Study on the Membrane Protein Conformational Changes and Mechanisms of Myocardial Cell Irradiated by Pulse Microwave
DENG Hua1, SONG Zhan-jun2, WANG De-wen3, YANG Hong1, MA Chun-quan1, LU Yu-kui1
Micro-Fourier transform infrared spectroscopy (FTIR) technique was applied to study the membrane protein conformational and functional changes of myocardial cell irradiated by pulse microwave. The results show that pulse microwave could influence the membrane protein structure markedly. The stretching vibration of lipid —CH2—, lecithoid CO, amide Ⅰ and Ⅱ region was decreased or displaced. The secondary structures of membrane protein were also changed by irradiation. The percentage of α-helix and β-pleated sheet structure decreased remarkably, and the disordering of secondary membrane proteins increased. All the above changes are correlated with the irradiation dosage. The results indicated that the integrality of myocardial cell membrane was injured by pulse microwave, and the membrane fluidity and stability decreased. Multi-biochemically active structures were damaged. Then all the changes could make a biochemical foundation of pathologic effects, which included membrane function decline, cell morphological change, configuration injuring and apoptosis etc. This paper is from a new view of protein conformation to explore the molecular pathologic mechanism of the damage caused by pulse microwave irradiation.
2006 Vol. 26 (08): 1437-1440 [Abstract] ( 1659 ) PDF (512 KB)  ( 440 )
1441 Wavelet Property Analysis of Near Infrared Spectra
TIAN Gao-you1, YUAN Hong-fu2,LIU Hui-ying1
Wavelet analysis (WT) was conducted on the simulated near infrared spectra (NIR) obtained by adding simulated background and simulated noise into diesel NIR. Results show that the background components are mainly located in the low frequency region, while noise in the high frequency region, and useful signal in the middle frequency region. Background and noise components can be simultaneously subtracted from the spectra by WT. The WT coefficients in mid-frequency details can be selected as variables to build the multivariate calibration model, which can improve analytic accuracy and reduce the analysis time.
2006 Vol. 26 (08): 1441-1444 [Abstract] ( 392 ) PDF (410 KB)  ( 390 )
1445 Fourier Transform Infrared Spectroscopic Study of Truffles
ZHAO De-zhang1, LIU Gang1*, SONG Ding-shan2, LIU Jian-hong3, ZHOU Yi-lan4, OU Jia-ming1, SUN Shi-zhong3
Truffles, which belong to ascomycetes, are rare wild growing edible mushrooms; their fruit body contains high nutritive value composition, and their polysaccharide constituents have potential medical applications. In the present paper, Fourier transform infrared (FTIR) spectroscopy was used for obtaining vibrational spectra of mushrooms of truffles growing in mountains of Yunnan province, southwest China. The results show that the mushrooms exhibit characteristic spectra. The two strongest absorption bands appear at about 1 077 and 1 042 cm-1, respectively. The spectra exhibit complicated patterns between 1200 and 750 cm-1, which may be used as fingerprints to discriminate different species of truffles. Great changes were also found between mold and healthy truffles, showing major differences observed in the bands of protein. In addition, some vibrational-spectrum differences were observed among the same species of truffles from different growing areas. It is showed that FTIR can provide valuable information about the chemical constituents of intact truffles prior to any extraction method is used.
2006 Vol. 26 (08): 1445-1448 [Abstract] ( 1501 ) PDF (442 KB)  ( 407 )
1449 Study on the Application of Absorption Spectrum to the Treatment of Landfill Leachate by Membrane Technology
ZHANG Hong-zhong1, FANG Shao-ming1, SONG Quan-yuan2, WANG Dian-zuo3
Molecular weight(MW) distributions of organics in the Bei Shenshu landfill leachate and their permeation from membranes were determined and studied by absorption spectrum, and the removal rates of organics with various molecular weight were measured. A new FTIR preparation method of wastewater sample used in the determination of landfill leachate was proposed in the present paper. The results showed that the aromatics in landfill leachate were mainly related to the the organics with MW＜2 500, whereas the distribution of total organics was dispersal comparatively. The removal rates of various humic compounds and oils in landfill leachate were estimated in accordance with the change in FTIR absorbance of permeation from membranes at three characteristic wave numbers 2 930, 2 960 and 3 030 cm-1, indicating that the defined membrane treatment process can removed organics with relevant MW fractions effectively.
2006 Vol. 26 (08): 1449-1453 [Abstract] ( 1213 ) PDF (804 KB)  ( 439 )
1454 The Pear Acidity Quantified Analysis Using PLS Methods and Fourier Transform Near-Infrared Spectroscopy
LIU Yan-de1, 2,YING Yi-bin1
Fourier transform near infrared(FT-NIR) spectrum of intact Xueqing pear was obtained by diffusion reflectance. Partial least squares (PLS) regression was carried out, describing the relationships between the data sets of laboratory data and the FT-NIR spectra. Different wave number ranges were chosen for regression and spectral information abstraction. The 3D-curves were shown for different factors, root mean square errors of cross validation (RMSECV), and prediction residual sum of squares (PRESS). Analysis results show that the best calibration model gave the relative high correlation coefficient of 0.79 and the low standard errors of prediction of 0.019 when the best wave number range was 5 452-12 285 cm-1 and the best factor was 7. The method of selecting advantageous wavelength ranges is feasible to obtain high prediction precision.
2006 Vol. 26 (08): 1454-1456 [Abstract] ( 1052 ) PDF (384 KB)  ( 441 )
1457 Near Infrared Determination of the Total Sugar in Chinese Ginsengs
LU Yong-jun1, QU Yan-ling1, CAO Zhi-qiang2, SONG Min1
In the present paper the analysis of the total content of sugar in Chinese ginseng was carried out by using near infrared spectroscopy, and in combination with the PLS algorithm the calibration analysis was performed at the same time. The result shows that the precision obtained through common chemical methods is almost the same as the one through NIR spectroscopy. The SEC(standard error of calibration) is 1.9% and the correlation coefficient is 0.951 7, which proves that this technique is well suitable for rapid quantitative determination of the total content of sugar in Chinese ginseng, and is simple, cheap, and without any chemical pollution, and can present real time result.
2006 Vol. 26 (08): 1457-1459 [Abstract] ( 1149 ) PDF (336 KB)  ( 427 )
1460 Determination of the Sequencing of Sealing and Writing with Fourier IR Microscopic Chemical Image System
YANG Sheng-jun1, ZOU Duo-sheng1, SHI Lin1,WANG Yan-jun1, XIE Jing-lan1,WANG Guo-qiang2
Using Fourier-transform IR microscopic chemical image system, combined with surface scan and reflection mode, noninvasive fast identification of the sequencing of writing and sealing was carried out. Results indicate that sealing prior to writing leads to evident characteristic peaks of the ink in the IR spectra, while writing prior to sealing results in clear characteristic peaks of the stamp-pad ink in the IR spectra. The method features speediness, exactness, impersonality, and non-invasion to the sample.
2006 Vol. 26 (08): 1460-1463 [Abstract] ( 1119 ) PDF (452 KB)  ( 400 )
1464 Water in Wood and Its Near Infrared Spectroscopic Analysis
JIANG Ze-hui, HUANG An-min*
Water is one of the most important characters of wood, so the effect of moisture content on the results must be considered in wood property analysis by NIR spectroscopy. In the present study, the absorbance of wood with various moisture content in NIR spectra was analyzed. Then the moisture contents in wood were predicted by NIR spectroscopy. The coefficient of determination is 0.99; and SEC and SEP are 0.041 and 0.043 respectively. In addition, a strong liner relationship between wood density with a 12% moisture content and wood NIR spectra with different moisture contents(7%，12%，20%，30% and 60%)was found. The results indicate that the model built with different moisture contents can predict wood density of 12% moisture content.
2006 Vol. 26 (08): 1464-1468 [Abstract] ( 1082 ) PDF (610 KB)  ( 476 )
1469 Study on the TLC-SERS of Sulfamethoxazole
ZHANG Jin-zhi1, WANG Yuan2, CHEN Xiang-ming1
The spectra of sulfamethoxazole, the efficient ingredient of sulfanilamide-like medicine, were investigated by combining the TLC and FT-Raman spectroscopy using the surface enhanced Raman spectroscopy technique. The result indicates that the main vibrant characteristic spectral band can be obtained by TLC in a samples of just about 1 μg. The difference between the compound sulfamethoxazole and the corresponding spectra picture is analyzed. The analysis shows that the method of combining TLC and FT-Raman spectroscopy by the SERS is practical and advantageous in the highly sensitive measurement of the chemical ingredient in medicine.
2006 Vol. 26 (08): 1469-1471 [Abstract] ( 1108 ) PDF (376 KB)  ( 438 )
1472 The SERS of Glycine Anhydride on Positive Silver Colloids
SI Min-zhen1, ZI Xing-fa1, WU Rong-guo2, ZHANG Peng-xiang3
The Raman spectrum and SERS of glycine anhydride in acidic and basic media on positive silver colloids were recorded. Calculations of the vibration frequencies were performed for glycine anhydride by B3LYP at 6-31g(d) level. The assignment of Raman spectrum and SERS of glycine anhydride in acidic media were obtained with the help of calculation. It was found that the glycine anhydride is physically adsorbed on the surface of silver. The SERS of glycine anhydride is very different in acidic media from that in basic media. This seems to mean that the geometry of glycine anhydride on positive silver colloid in basic media has changed.
2006 Vol. 26 (08): 1472-1475 [Abstract] ( 1160 ) PDF (402 KB)  ( 444 )
1476 Studies of Interference Source in Laser Raman Spectra of Near Infrared
LIU Wen-han, MAO Xin-biao, MA Chun-an
Many abnormal peaks in laser Raman spectra were found in determinations with laser illuminant of 785.18 nm. After studies, it was found that the notch filter caused the interferences and the characteristics of the band pass filter were not good. The absorption spectral characteristics of notch filter were studied for the new and old of band pass filters.
2006 Vol. 26 (08): 1476-1478 [Abstract] ( 1626 ) PDF (299 KB)  ( 466 )
1479 Spectral Analysis of Transient Species of Quinoline Degradation
WANG Shi-long1, ZHU Da-zhang1, SUN Xiao-yu1, SHI Jun1, NI Ya-ming1,WANG Wen-feng2,YAO Si-de2
Quinoline’s degradation was studied by pulse radiolysis. It was found that hydrated electron, hydrogen radical, hydroxyl radical and SO·-4 can react with quinoline. The absorption spectra of the transient species of quinoline were obtained, and related constants were determined, but it was found that the species of Br·-2 and N·-3 can’t react with quinoline. The results give us some advices for studing the degradation of quinoline.
2006 Vol. 26 (08): 1479-1482 [Abstract] ( 434 ) PDF (490 KB)  ( 451 )
1483 Study of the pH Sensitive Polymer
GUO Liang-qia, XIE Zeng-hong*, LIN Xu-cong, ZHENG Xiang-hua, ZHANG Mao-sheng, CHEN Guo-nan
A pH sensitive polymer was prepared by copolymerization of methacrylic acid as monomer, diethylene glycol dimethacrylate as cross-linking reagent, heptane as porogen, and fluorescent dye eosin as indicator. The factors of influence on the preparation, and the character of the pH sensitive polymer for pH were studied. The maximal emission wavelength of eosin was red shifted in the polymer than in solution, the apparent Ka largened, and the dissociation equilibrium of indicator was shifted to acidity direction, because the polarity of polymer diminished. Under the optimal condition, the calibration curve of the pH sensitive polymer covered the range of pH 0-3.0 with good reproduction and reversibility.
2006 Vol. 26 (08): 1483-1486 [Abstract] ( 1624 ) PDF (530 KB)  ( 396 )
1487 Immune Resonance Scattering Spectral Method for the Determination of Trace IgG
DENG Jun-yao1，3, SUN Shuang-jiao1, JIANG Zhi-liang1，2*, LIANG Ai-hui2
In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 μg·mL-1. The detection limit is 0.99 μg·mL-1. The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.
2006 Vol. 26 (08): 1487-1489 [Abstract] ( 590 ) PDF (340 KB)  ( 383 )
1490 Influence of Matrix on the Optical Limiting Effect of Metallophthalocyanine Doped Composite
WANG Cheng-yong, FENG Miao, ZHEN Xiao, ZHAN Hong-bing*
Nickle tetrasulfo-phthalocyanine (NiTSPc) and Lead phthalocyanine (PbPc) are introduced respectively into silica gel glass matrix by sol-gel technique. Homogeneously doped composites are produced, and optical limiting examination was carried out. The results show that the change in matrix composition, structure and sol-gel process will influence the chemical micro-environment and excited state physical process of doped metallo phthalocyanine, resulting in the change in composite’s optical limiting behaviors.
2006 Vol. 26 (08): 1490-1493 [Abstract] ( 449 ) PDF (526 KB)  ( 452 )
1494 Studies on the Reaction of Balofloxacin with Bovine Serum Albumin
YAN Zheng-yu, SHAO Xiu-fen, JIANG Xin-min, HU Yu-zhu
In the present paper, a fluorescence method was used to study at different pH the fluorescence quenching of bovine serum albumin (BSA) by its interaction with balofloxacin (BLFX). The interaction association constants of BSA and BLFX were determined from a double reciprocal line Weaver-Burk plot. According to the Frster dipole-dipole energy transfer, the distance to be measured between the BLFX and tryptophane is 5.09 nm. From thermodynamical coordination it can be judged that the binding power between BLFX and BSA is electrostatic effect. The effect of BLFX on the conformation of BSA was also analyzed by using synchronous fluorescence spectroscopy.
2006 Vol. 26 (08): 1494-1498 [Abstract] ( 1101 ) PDF (600 KB)  ( 399 )
1499 An Analysis of Quenching Characteristics of DOM in Water by Laser Induced Fluorescence
ZHAO Nan-jing, LIU Wen-qing, ZHANG Yu-jun, CUI Zhi-cheng, LIU Jian-guo, LI Hong-bin, WEI Qing-nong, YANG Li-shu
In the experiment an excitation resource at 355 nm from a third harmonic Q-switched Nd:YAG laser was used, and the commercial humic acid of different concentrations was used as the research samples. The fluorescence spectrum of dissolved organic matter (DOM) in combination with laser-induced fluorescence (LIF) measurements was measured in the laboratory, and the characteristics of fluorescence quenching of DOM were analyzed. The results show that the intensity of water Raman scattering and DOM fluorescence was reduced gradually and increased linearly respectively with raising the concentration of humic acid. The water Raman scattering signal was absorbed almost completely by the ground state molecules of DOM at 40 mg·L-1, and the fluorescence intensity of DOM reached a maximum at 16 mg·L-1 and decreased slowly with further increasing the concentration of humic acid. Consequently, we can achieve better monitoring of DOM in water through analyzing the characteristics of fluorescence quenching at different concentrations of humic acid.
2006 Vol. 26 (08): 1499-1502 [Abstract] ( 1526 ) PDF (477 KB)  ( 493 )
1503 Synthesis and Spectral Studies of Functionalized L-Cys-CdS Nanoparticles as Fluorescence Probes
DAI Mei-ling1, YAN Zheng-yu1*, PANG Dai-wen2, SHAO Xiu-fen1, QU Ping1, ZHAO Jing-tian1
In this work, nano-CdS was successfully prepared. The nano-CdS was also modified with L-cysteine. Absorption and fluorescence spectra of CdS nanoparticles for different pH values, reaction times and different Cys/Cd2+/S ratios were investigated. Meanwhile a fluorescence enhancing effect was observed between trace zinc ions and the functionalized L-Cys-CdS nanoparticles. The response is linearly proportional to the concentration of zinc ions from 1.0 to 15 μmol·L-1. The functionalized nanoparticles are hopeful of use as fluorescence probes in detecting trace elements in biological samples.
2006 Vol. 26 (08): 1503-1507 [Abstract] ( 1681 ) PDF (540 KB)  ( 505 )
1508 Fluorescence Study on the Interaction of Alizarin Yellow R and Bovine Serum Albumin in Alkali Solution
ZHANG Huan1, WANG Xing-ming1*, WANG Qing-cheng1, SHI Rong-ming1, DING Li-sheng2
The interaction of alizarin yellow R(AYR) and bovine serum albumin (BSA)was investigated by fluorescence method in alkali buffer solution. It was shown that AYR had a powerful ability to quench the BSA fluorescence at excitation and emission wavelengths of λex=393 nm and λem=641 nm in the medium solution of pH 11.00, and there were five binding sites of AYR to BSA;The combination reaction of AYR with BSA was a static quenching process, and from the effects of temperature on the fluorescence quenching rate of AYR-BSA and the Stern-Volmer quenching constant (KSV) and the Lineweaver-Burk quenching constant (KLB), the binding constant was calculated to be K=1.6×104 L·mol-1;as the enthalpy change ΔHθ＜0 and entropy change ΔSθ＜0, and AYR has an ability to quench the BSA-CBBG fluorescence, it can be deduced that the Van der Walls force and hydrogen bond are the main binding forces between AYR and BSA.
2006 Vol. 26 (08): 1508-1512 [Abstract] ( 1147 ) PDF (566 KB)  ( 435 )
1513 Study of Cancer Cells Fluorescence Lifetime Based on Picosecond Time Resolution
CHEN Bi-fang1,LIU Tian-fu2
The object of the present study was the ultrafast photodynamic processes of hematoporphyrin derivative (HPD) for diagnosis and therapy of cancer. Time-resolved fluorescence spectra of cancerous and normal cells were measured using an ultrashort pulse laser spectral technique and picosecond time-correlated single-photon counting system. The fast part of cancerous and normal cells fluorescence decay was approximately 150 and 300 ps, the fluorescence peak intensity of cancerous and normal cells decayed about 10% and 55% in 12 hour, the lifetime of cancerous and normal cells was about 824 and 1 798 ps by calculating date of fluorescence decay, and HPD stay time was about 17 and 6 days in the cancerous and normal cells sample respectively. The data show that cancerous cells were greatly intimate with HPD. The results obtained can be used as an important basis for the diagnosis of cancer based on ultrashort pulse laser spectral technique. The results will contribute to feebleness ultrafast fluorescence of biology sample for real time measurement.
2006 Vol. 26 (08): 1513-1515 [Abstract] ( 1589 ) PDF (377 KB)  ( 404 )
1516 Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application
HE Hua1,WANG Ling-li1,LIU Xu-hui2,JIAO Qing-cai3,CHUONG Pham-Huy4,WANG Guang-ji1*
On binding to deoxyribonucleic acid, the complex of terbium-ciprofloxacin (Tb3+-CIP) increases its fluorescence quantum efficiency. Based on this, an easy, rapid and sensitive method for the determination of DNA was developed. Like ethidium bromide (EB), the complex can be intercalated into DNA bases, but it is non-poisonous. Determination can be made at pH 7.0, where the native structure of DNA is not disrupted. The maximum emission is at 545 nm with excitation at 325 nm. This method has good sensitivity (2.8×10-9 mol·L-1 of ctDNA), high selectivity and a wide linear range (4..3×10-7-3.0×10-5 mol·L-1 of ctDNA). This complex was also employed for the clinical examination of DNA in whole blood of tumor patients. Ten tumor patients and 10 normal persons were enrolled in this study. It was showed that significant difference existed between control group and tumor group (p＜0.05).
2006 Vol. 26 (08): 1516-1519 [Abstract] ( 1284 ) PDF (490 KB)  ( 460 )
1520 Fluorimetric Determination of Trace Ruthenium in Anode Slime of Alkali Chloride Factory with 1,10-Phenanthroline
KAI Xiao-ming1, ZHAN Feng2
The complex reaction of ruthenium with 1,10-phenanthroline was studied. In the medium of pH 5 HAc-NaAc buffer and NH2OH·HCl solution, ruthenium reacts with 1,10-phenanthroline to form a 1∶3 complex by heating in a boiling water bath for 5 min. The fluorescence peak excitation wavelength is 448 nm, and the emission peak wavelength is 586 nm. The linear relation between fluorescence intensity and the concentration of Ru(Ⅱ) was found to be 0-10 μg·25 mL-1. The ruthenium in the sample was separated by distill. The method has been used to the determination of ruthenium in anode slime of alkali chloride factory with satisfactory results.
2006 Vol. 26 (08): 1520-1522 [Abstract] ( 1340 ) PDF (331 KB)  ( 381 )
1523 Fluorescence Spectra and Fluorescence Quantum Yield of Triton X-100
ZHAO Jing, WEI Yong-ju*
Fluorescence spectra and fluorescence quantum yield of triton X-100 (TX) aqueous solution are reported. In strong acidic solutions, TX gives no fluorescence. When pH＞1, TX gives a strong and steady fluorescence with the maximum excitation wavelengths at 229 and 275 nm, and the maximum emission wavelength at 302 nm, respectively. TX aqueous solution can produce resonance fluorescence. The resonance fluorescence peak is located at 285 nm. A linear relationship between the fluorescence intensity and TX concentration was found in the range of 0.1-90 mg·L-1. The detection limit of TX is 0.1 mg·L-1. By using L-tryptophan as a reference, the fluorescence quantum yield of TX at maximum excitation wavelength 280 nm was measured to be 0.121.
2006 Vol. 26 (08): 1523-1525 [Abstract] ( 1157 ) PDF (336 KB)  ( 428 )
1526 Quantum Chemistry Study on Fluorescence Spectra of Four Flavonoid Compounds
LIAO Xian-wei1, SU Yu2, LIU Shan1, DENG Jia-li1
The semi-empirical method PHF/PM3 of quantum chemistry was used for studying the fluorescence spectra of four flavonoid compounds. Firstly, the geometries of these four compounds were optimized by energy gradient method. The results show that in these four copmounds, the two six-membered rings on the left are in the same plane, but these are different torsion angles between this plane and the benzene-ring plane on the right. Secondly, vibrational analyses were performed for the four optimized configurations. There is no imaginary frequency in vibrational analyses. This indicates that the optimized configurations are essentially reasonable. Finally, on this basis, the electronic spectra were calculated by CIS method. All the calculated results are basically consistent with experimental values.
2006 Vol. 26 (08): 1526-1529 [Abstract] ( 1061 ) PDF (403 KB)  ( 400 )
1530 Determination of Metacycline in Mixture Sample by Synchronous-Derivative Fluorimetry
AODENG Gao-wa, ZHANG Ying, FAN Hai-yan
A new synchronous-derivative fluorimetric method for the determination of metacycline(MTC) in the presence of oxytetracycline(OTC) is proposed. The degradation products of MTC in 0.1 mol·L-1 NaOH are strongly fluorescent. The addition of cetyltrimethylammonium bromide(CTMAB) enhances the fluorescence intensity 2-fold. The synchronous fluorescence spectra of MTC and OTC degradation products do not interfere with each other under the condition that Δλ=100 nm. MTC can be determined in the presence of OTC in the range of OTC+MTC ratio of 1+116-1+1. The recoveries of MTC in the mixture sample are in the range of 94%-102%.
2006 Vol. 26 (08): 1530-1532 [Abstract] ( 1534 ) PDF (382 KB)  ( 365 )
1533 Study on Absorption Spectra of Malignant Plasma of Esophagus
ZHANG Xiao-dong1, ZHAO Yuan-li2, GUO Tao3, WANG Lei-ming4, GE Xiang-hong2, LUO Lei2
There are too many articles about diagnosing cancers by the content of tumor chemical signs in plasma, but there are few articles about diagnosing them by spectroscopy. The present article belongs to the few. The authors can differentiate the normal and malignant esophagus by the statistic and analysis of absorption spectra after their plasmas are detected by spectrophotometry, which provide a new approach to quickly diagnosing malignant esophagus.
2006 Vol. 26 (08): 1533-1535 [Abstract] ( 1051 ) PDF (400 KB)  ( 419 )
1536 Determination of Trace Manganese by Catalytic Kinetic Spectrophotometry Method with CTMAB as the Sensitizer
SONG Gui-lan, REN Hao, LI Guo-bao, YANG Lin
A new catalytic spectrophotometric method for the determination of trace amount of Mn(Ⅱ) was developed. The method is based on the oxidation of 7-(2-nitryl-4-methyl phenylazo)-8-hydroxy-qinolin-5-slfonic acid by potassium periodate in the presence of nitrilo triacec acid as an activator and CTMAB as a sensitizer. The wavelengths for the determination was 509 nm.The linear range of determination is 0-3.2×10-9 g·mL-1 for Mn(Ⅱ). The determination limit is 3.8×10-11 g·mL-1. The interference of foreign ions was studied. The method can be used for the determination of Mn(Ⅱ) in mung bean, rice, cereal and soil samples.
2006 Vol. 26 (08): 1536-1539 [Abstract] ( 1707 ) PDF (400 KB)  ( 425 )
1540 Application of ICP-AES to the Chemical Speciation of Heavy Metals in Flyash
GUO Yu-wen1, 3,PU Li-mei1, QIAO Wei1, WANG Wei1*, WAN Xiao1, ZHANG Xin-rong2
Chemical speciation of seven heavy metals of flyashes in incinerator was quantitatively tested using ICP-AES. Results showed that ICP-AES procedure could carry out quick, exact and high precision experiments. RSD ratio for most detected metals was lower than 3% while few metals present a comparatively high RSD when whose content was near the detection limits. The recovery ratio was 85.7%-100.63% flyashes were found to have high content of Zn, Pb. Cd, Cu, Mn, Pb and Zn existed mostly as carbonates and were leachable, while Cr and Ni were combined to metal oxides substrates and present immobilization characteristics.
2006 Vol. 26 (08): 1540-1542 [Abstract] ( 1021 ) PDF (360 KB)  ( 416 )
1543 Determination of Elements in Euonymus Alatus by Microwave Digestion-ICP-AES
TANG Rui, HOU Xiang-yan, SUN Chen, YANG Yi*
The determination of elements in Euonymus alatus by ICP-AES was carried out in the present paper. The Euonymus alatus samples were digested by microwave digestion technique. The optimized conditions of microwave digestion procedure obtained by orthogonal array design were as follows: the volume ratio of HNO3-HCl is 2∶1; the ratio of liquid/solid is 100∶1; the sample particle size is 100-140 mesh. ICP-AES method was applied to the determination of ten trace elements in the sample, and was compared with AAS. The results showed that the method was rapid and simple with the relative standard deviations between 0.5%-6.2%, and the recovery between 85.5% and 105.6%.
2006 Vol. 26 (08): 1543-1546 [Abstract] ( 427 ) PDF (451 KB)  ( 370 )
1547 Determination of Molybdenum in Supported Catalysts by ICP-AES
FANG Yi-wen1, 2, DONG Xin-fa1, WANG Wen-jin2, XUE Liang2, LIN Wei-ming1
The supported Mo catalysts were pretreated using hydrofluoric acid. Then Mo was determined by inductively coupled plasma atomic emission spectrometry(ICP-AES). In the present method, the detection limit of Mo was 8.220 ng·mL-1, the recovery of standard addition was 102.6%-104.3%, the relative standard deviation(n=11) was less than 0.860%. The method is efficient, accurate, and easy to operate. Molybdenum in supported catalysts, which were prepared under different conditions, was determined by this method. The results showed that the content of Mo in supported catalysts decreased after preparation. The decreases in the content of Mo were different when the active component concentrations in impregnation solution, and the calcination temperature, changed.
2006 Vol. 26 (08): 1547-1549 [Abstract] ( 1030 ) PDF (312 KB)  ( 414 )
1550 Determination of Fatty Acids and Organic Acids in Ranunculus Ternatus Thunb Using GC-MS
CHEN Jun1, YAO Cheng1*, XIA Li-ming1, OUYANG Ping-kai2
The determination of fatty acids and organic acids in Chinese medicinal plant Ranunculus ternatus Thunb using GC-MS was studied. The Ranunculus ternatus Thunb from Henan province was cut into less than 20 mesh pieces, then extracted by petroleum ether or ether in refluxing and esteried, and finally was determined using GC-MS. The results show that there are 23 kinds of organic compounds in the Chinese medicinal plant Ranunculus ternatus Thunb from Henan, among which 15 kinds of fatty acids were identified, including myristic acid, palmitic acid, stearic acid, oleic acid, linolenic acid, eicosanoic acid, docosanoic acid etc. The unsaturated fatty acids and oleic acid account for 58.19% and 35.68% of the total organic compounds respectively. The kinds of fatty acid in petroleum ether extract and ether extract are the same.
2006 Vol. 26 (08): 1550-1552 [Abstract] ( 1068 ) PDF (437 KB)  ( 380 )
1553 Determination of Trace Arsenic and Mercury in Antimony Concentrates by Hydride Generation Atomic Fluorescence Spectrometry
YUAN Ai-ping1, TANG Yan-xia1, HUANG Yu-long1, WANG Jing-ling1, QIN Ran1, WU Jian-ling2, MENG Wen-fei2
A method for the determination of trace arsenic and mercury in antimony concentrates by hydride generation atomic fluorescence spectrometry was investigated. The method of dissolving sample and the effect of concomitant ion were studied. It was found that aqua regia can be used to dissolve the sample completely, with maximum quantities of dissolved arsenic and mercury. The interference of chemical elements in antimony concentrates was avoided by adding thiourea-Vc. The matrix of antimony was vaporized with tartaric acid during sample pre-treatment. Trace arsenic and mercury were determined in real samples. The detection limits of 0.220 ng·mL-1 and 0.002 ng·mL-1 were obtained, the relative standard deviations(RSD%=11) are 1.47% and 0.52%, and the recoveries are 94.0%-103.0% and 98.7%-102.8% for As and Hg respectively. This method has proved rapid, accurate and highly sensitive.
2006 Vol. 26 (08): 1553-1556 [Abstract] ( 512 ) PDF (521 KB)  ( 411 )
1557 Study on Cadmium Absorption in Pumpkin by Atomic Absorption Spectrophotometry
LI Zhen-xia1, JING Rui-jun1, DONG Wei-hua2, LI Xin-zheng1,LIU Hong1
A study was carried out on the characteristic of cadmium absorption in pumpkin by atomic absorption spectrophotometer. The results show that the cadmium absorption amount in pumpkin increased with the increase in cadmium concentration. Meanwhile the cadmium absorption amount in pumpkin increased with time. Eight hours after being cultured in the liquid, the cadmium absorption amount became saturated. The cadmium absorption rate reached the peak after 2 hours, then the absorption rate gradually reduced. The cadmium absorption amount in pumpkin is less in acid or alkali compared with neutral condition. And the absorption amount became minimum in pH 3, while maximum in pH 7.
2006 Vol. 26 (08): 1557-1559 [Abstract] ( 1087 ) PDF (389 KB)  ( 387 )
1560 Study on Colored Drawing Technique of Qionglai Kiln by SRXRF
LUAN Tian, MAO Zhen-wei, WANG Chang-sui
The colored drawing technique of Qionglai kiln is a debate problem in the archaeology and ancient ceramic research. In the present paper, SRXRF linescan technology was used to study the distribution mode of the colorific elements on the cross-sections of samples. The analytical result indicates that there existed two kinds of decoration techniques of colored drawing, i.e. the techniques of the underglaze color and oveglaze color coexisted in that time.
2006 Vol. 26 (08): 1560-1563 [Abstract] ( 1112 ) PDF (721 KB)  ( 416 )
1564 Spectral Properties and Thermal Stability of Zinc Phosphate Hydrate
YUAN Ai-qun1，2, HUANG Zeng-wei1, DENG Guang-hui1, SONG Bao-ling2,TONG Zhang-fa1
X-ray, FTIR, and Raman spectra were used to measure the spectral properties of three kinds of zinc phosphate hydrate. Spectral changes with crystalline water were analyzed. The thermal stability of zinc phosphate tetrahydrate was studied by TG-DTA to identify the existent temperature of zinc phosphate hydrate. The results show that the differences in crystalline water among these hydrates results in the variation in both 2θ characteristic values and peaks. The FTIR spectra reflect H—O—H strength information (1 600 cm-1) and O—H bond stretching vibrations (3 400-3 500 cm-1). Raman spectra show the difference in P—O bond stretching mode at 400-700 cm-1 and the shape of O—H stretching peak. According to the mass-loss curves, the onset temperature of zinc phosphate tetrahydrate was 95 ℃. Heating to 145 ℃ was accompanied by the removal of 2H2O and transform into Zn3(PO4)2·2H2O, indicating that Zn3(PO4)2·2H2O was established at this temperature. Zn3(PO4)2·H2O can be obtained by heating to 195 ℃. The third stage of dehydration gave an anhydrous Zn3(PO4)2 phase.
2006 Vol. 26 (08): 1564-1567 [Abstract] ( 1178 ) PDF (426 KB)  ( 448 )

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