| 光谱学与光谱分析 |
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| Immune Resonance Scattering Spectral Method for the Determination of Trace IgG |
| DENG Jun-yao1，3, SUN Shuang-jiao1, JIANG Zhi-liang1，2*, LIANG Ai-hui2 |
1. Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China
2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China
3. Children-Women Health Hospital of Guilin City, Guilin 541001, China |
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Abstract In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 μg·mL-1. The detection limit is 0.99 μg·mL-1. The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.
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Received: 2005-03-28
Accepted: 2005-06-28
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Corresponding Authors:
DENG Jun-yao
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| Cite this article: |
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DENG Jun-yao,SUN Shuang-jiao,JIANG Zhi-liang, et al. Immune Resonance Scattering Spectral Method for the Determination of Trace IgG[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1487-1489.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I08/1487 |
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