| 光谱学与光谱分析 |
|
|
|
|
|
| Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application |
| HE Hua1,WANG Ling-li1,LIU Xu-hui2,JIAO Qing-cai3,CHUONG Pham-Huy4,WANG Guang-ji1* |
1. China Pharmaceutical University，Nanjing 210009, China
2. INSERM U-602，Institut du Cancer et d’Immunogénétique (ICIG)，Hopital Paul Brousse，94807，Villejuif，France
3. Pharmaceutic Biotechnology Key Laboratory，College of Life Science，Nanjing University，Nanjing 210093，China
4. Faculty of Pharmacy, University of Paris V, 4 Avenue de l’Observatoire, 75006, Paris, France |
|
|
|
|
Abstract On binding to deoxyribonucleic acid, the complex of terbium-ciprofloxacin (Tb3+-CIP) increases its fluorescence quantum efficiency. Based on this, an easy, rapid and sensitive method for the determination of DNA was developed. Like ethidium bromide (EB), the complex can be intercalated into DNA bases, but it is non-poisonous. Determination can be made at pH 7.0, where the native structure of DNA is not disrupted. The maximum emission is at 545 nm with excitation at 325 nm. This method has good sensitivity (2.8×10-9 mol·L-1 of ctDNA), high selectivity and a wide linear range (4..3×10-7-3.0×10-5 mol·L-1 of ctDNA). This complex was also employed for the clinical examination of DNA in whole blood of tumor patients. Ten tumor patients and 10 normal persons were enrolled in this study. It was showed that significant difference existed between control group and tumor group (p＜0.05).
|
|
Received: 2005-04-28
Accepted: 2005-09-13
|
|
|
|
Corresponding Authors:
WANG Guang-ji
|
|
| Cite this article: |
|
HE Hua,WANG Ling-li,LIU Xu-hui, et al. Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1516-1519.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2006/V26/I08/1516 |
[1] Shawn D W，David O W，Jeremy S L. J. Inorg. Biochem.，2003，94：94.
[2] Vasil B，Irene K，Jemal C, et al. Inorg. Chim. Acta，2002，339：145.
[3] Song Yumin，Kang Jingwan，Zhou Jing, et al. Spectrochim. Acta Part A，2000，56：2491.
[4] BIAN Wei, WEI Yu-xia, WEI Yan-li(卞 伟，魏玉霞，卫艳丽，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1)：125.
[5] Geyer C Ronald，Dipankar S. J. Mol. Biol.，1998，275: 483.
[6] Bohuslav R，Alena S，Martin F, et al. J. Chromatogr. B，2004，800：169.
[7] Alla Y，Alexander K，Axel D, et al. Spectrochim. Acta Part A，2005，61：109.
[8] Zhou Jing，Wang Liu-fang，Wang Jin-yi, et al. J. Inorg. Biochem.，2001，83：41.
[9] Andoh T. Biochimie.，1998, 80：235.
[10] Andrew J G，Ian S B. J. Pharm. Biomed. Anal.，2000，22：849.
[11] Shelley R M，Clive L G. Radiat. Phys. Chem.，2005，72：287.
[12] Mark A S，Scott W M. J. Mol. Biol.，1997，266：927.
[13] Takashi E，Naoki S，Isao T, et al. Biochem. Biophys. Res. Comm.，2002，290：546.
[14] Anatoly I D，Janet K，Christopher R, et al. J. Mol. Biol.，2003，331：795.
[15] Elizebeth C T，Charlotte H C，Chris J W, et al. Chem. Biol.，2004，11：69.
[16] WU Shu-qing，CAO Qiu-e，ZHAO Yun-kun, et al(吴淑清，曹秋娥，赵云昆, 等). Chinese J. Anal. Chem.(分析化学)，2000，28(12)：1462.
[17] YANG Jun，REN Yu，XU Yi-zhuang, et al(杨 军，任 宇，徐怡庄, 等). Chem. J. Chinese Universities(高等学校化学学报) ，2004, 25：243.
[18] LONG Yun-fei, CHEN Xiao-ming, CAI Tie-jun(龙云飞，陈小明，蔡铁军). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(5)：610.
[19] Polyanichko A M，Andrushchenko V V，Chikhirzhina E V，et al. Nucleic Acids Res.，2004，32(3)：989.
[20] SONG Gong-wu，HE Zhi-ke，ZENG Yun-peng，et al (宋功武，何治柯，曾云鹏, 等). Chinese J. Anal. Chem.(分析化学)，1999，27(10)：1183.
|
| [1] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
| [2] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
| [3] |
LENG Jun-qiang, LAN Xin-yu, JIANG Wen-shuo, XIAO Jia-yue, LIU Tian-xin, LIU Zhen-bo*. Molecular Fluorescent Probe for Detection of Metal Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2002-2011. |
| [4] |
QIAN Duo, SU Wen-en, LIU Zhi-yuan, GAO Xiao-yu, YI Yu-xin, HU Cong-cong, LIU Bin, YANG Sheng-yuan*. Soy Protein Gold Nanocluster as an “Off-On” Fluorescent Probe for the Detection of Bacillus Anthracis Biomarkers DPA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1815-1820. |
| [5] |
ZHENG Li-zhen1, 2, CHENG Cong2, MA Wen-hua2, WANG Zhuo-rui2, HU Dao-dao2*. Online Detection of Water Forms and Moisture Volatilization Behavior in Earthen Relics Based on FE Fluorescence Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 383-388. |
| [6] |
LÜ Chun-qiu1, SI Lu-lu1, PAN Zhao-jin2, LIANG Yang-lin1, LIAO Xiu-fen2, CHEN Cong-jin2*. Fast and Ratiometric Detection of Dimethoate Via the Dual- Emission Center Nitrogen-Doped Carbon Quantum Dots[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 468-474. |
| [7] |
ZHAI Yan-ke1, PAN Yi-xing1, XIANG Hao1, XU Li1*, ZHU Ze-ce2, LEI Mi1. Coordination Interaction of DSAZn With Quercetin and High Sensitivity Detection of Quercetin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 122-128. |
| [8] |
SONG Jiang-tao, YUAN Yue-hua, ZHU Yong-jun, WANG Yu-zhen, TIAN Mao-zhong*, FENG Feng*. Research Progress of Near-Infrared Fluorescent Probes for Hydrogen Sulfide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3321-3329. |
| [9] |
ZHANG Fei1, 5，HUA Xia2*，YUAN Jia-ying3，YOU Fan1，YE Ren-cai4，DING Li4, ZHAO Jian-mei4. Determination of Thallium in Blood of Occupational Exposed Population by Inductively Coupled Plasma Mass Spectrometry With High Matrix Introduction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2870-2874. |
| [10] |
XU Yi-fei, LIU Lu, SHI Shi-kao*, WANG Yue, PAN Yu-jing, MA Xing-wei. Spectroscopic Properties of Carbon Quantum Dots Prepared From Persimmon Leaves and Fluorescent Probe to Fe3+ Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2418-2422. |
| [11] |
WAN Xiao-ming1, 2, ZENG Wei-bin1, 2, LEI Mei1, 2, CHEN Tong-bin1, 2. Micro-Distribution of Elements and Speciation of Arsenic in the Sporangium of Pteris Vittata[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 478-482. |
| [12] |
SUN Dai-qing1, 2, XIE Li-rong1*, ZHOU Yan2, GUO Yu-tao1, CHE Shao-min2. Application of SG-MSC-MC-UVE-PLS Algorithm in Whole Blood Hemoglobin Concentration Detection Based on Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2754-2758. |
| [13] |
LI Meng-yao1, 2, WANG Shu-ya1, XIE Yun-feng1, LIU Yun-guo3*, ZHAI Chen1*. Detection of Protease Deterioration Factor in Tomato by Fluorescence Sensor Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3477-3482. |
| [14] |
JIA Hui-jie, ZHU Ning, GAO Yuan-yuan, WANG Ya-qi, SUO Quan-ling*. Effect of Substituent Structure of Benzothiazole Probe on Recognition to Metal Ion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3594-3598. |
| [15] |
ZHU Dan-dan1, 2, QU Peng2*, SUN Chuang2, YANG Yuan2, LIU Dao-sheng1*, SHEN Qi3, HAO Yuan-qiang2*. A Benzothiazole-Based Long-Wavelength Fluorescent Probe for Dual-Response to Viscosity and H2O2[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1775-1779. |
|
|
|
|
