|
|
|
|
|
|
Coordination Interaction of DSAZn With Quercetin and High Sensitivity Detection of Quercetin |
ZHAI Yan-ke1, PAN Yi-xing1, XIANG Hao1, XU Li1*, ZHU Ze-ce2, LEI Mi1 |
1. School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430072, China
2. School of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan 430200, China
|
|
|
Abstract Quercetin is a natural flavonoid compound that is used for the prevention and treatment of hypertension, hyperlipidemia, cardiovascular disease, cancer, etc. Therefore, quantitative detection of quercetin was particularly important in biochemistry and clinical medicine. A highly selective and sensitive detection methodfor quercetin with AIE (aggregation-induced luminescence phenomenon) fluorescent molecules was proposed, which identified the target molecule quercetin through coordination interaction with excited state electron transfer. The fluorescence of DSAZn in PBS buffer at pH 7.0 with adding five drug molecules (quercetin, icaritin, isorhamnetin, rutin and dopamine) was studied.The fluorescence emission spectra of 435~680 nm were scanned by a fluorescence spectrophotometer with an excitation wavelength of 415 nm. The ultraviolet absorption spectrum of 250~750 nm was scanned by ultraviolet spectrophotometer, which showed that the traditional Chinese medicine molecule quercetin could form a complex with the AIE fluorescent probe and thusstaticallyquenchedthe fluorescence of the AIE probe. Fluorescence detection showed that the quenching effect of five drug molecules on the fluorescent probe was significantly different. The binding constant of quercetin and DSAZn was 1.34×107 L·mol-1, which was an order of magnitude higher than that of the other four drug molecules, exhibitinga good selectivity for quercetin. The detection limit of quercetin was 3.07 nmol·L-1, which was lower than the value reported in many kinds of literature, exhibiting a high sensitivity for quercetin. The titrationcurve equation of quercetin to DSAZn based on the fluorescence titration spectrum was: y=0.013 4x-0.294 82. The linear range of quercetin concentration was 0~5 μmol·L-1 with a linear correlation coefficient of 0.994 3. Thus, a highly selective and sensitivedetection method for quercetin by AIE-type fluorescent molecules was constructed. This method was simply operated and repeatable, which provided a new research idea for detecting drugs with similar structures.
|
Received: 2021-10-22
Accepted: 2022-04-21
|
|
Corresponding Authors:
XU Li
E-mail: xuli81819009@126.com
|
|
[1] ZHANG Zhi-qin, ZHU Shuang-xue(张志琴, 朱双雪). Pharmaceutical Research(药学研究), 2013, 32(7): 400.
[2] WU Yu-xiang, WANG Xiao-ping, WANG Hong, et al(武玉祥, 王小平, 王 虹, 等). Journal of Anhui Agricultural Sciences(安徽农业科学), 2016, 44(13): 164.
[3] ZHANG Lan, LIU Yuan-huan, HE Yu, et al (张 兰, 刘远环, 何 聿, 等). Analysis and Measurement Technology & Instrument(分析测试技术与仪器), 2005, 11(1): 22.
[4] WU Shui-hua(吴水华). China Modern Chinese Medicine(中国现代中药), 2012, 14(8): 10.
[5] HU Yu-fei, FENG Ting, LI Gong-ke(胡玉斐, 冯 婷, 李攻科). Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy(光谱化学学报A辑: 分子与生物分子光谱), 2014, 118: 921.
[6] Wang Zhongxia, Gao Yuanfei, Jin Xing, et al. The Analyst, 2019, 144(7): 2256.
[7] Sachin Kadian, Gaurav Manik. Food Chemistry, 2020, 317: 126457.
[8] QIU Li-juan, MENG Xin, HOU Sheng-shu, et al(邱丽娟, 孟 欣, 候升书, 等). Journal of Pharmaceutical Practice(药学实践杂志), 2018, 36(1): 50.
[9] JIA Bao-xiu, GU Zhi-liang, LI Yu-qin, et al(贾宝秀, 谷志亮, 李玉琴, 等). Chinese Journal of Modern Applied Pharmacy(中国现代应用药学), 2014, 31(1): 17.
[10] LIN Tian-le, YAN Bao-zhen, HU Gao-fei(林天乐, 严宝珍, 胡高飞). Chinese Journal of Analytical Chemistry(分析化学), 2006, 34(8): 1125.
[11] Rubens F V de Souza, Wagner F De Giovani. Redox Report, 2004, 9(2): 97.
[12] Kruppa M, König B. Chemical Reviews, 2006, 106:3520.
[13] WU Ya-jun, CHEN Hong-ling(吴亚军, 陈洪龄). Journal of Nanjing University of Technology·Natural Science(南京工业大学学报·自然科学版), 2021, 43(1): 47.
[14] Chris Wai Tung Leung, Hong Yuning, Chen Sijie, et al. Journal of the American Chemical Society, 2013, 135:62.
[15] Dan Ding, Li Kai, Liu Bintan, et al. Accounts of Chemical Research, 2013, 46: 2441.
[16] Zhu Zece, Zhou Jie, Li Zheng, et al. Sensors and Actuators B: Chemical, 2015, 208: 151.
[17] Mora A, Payá M, Ríos J L,et al. Biochem. Pharmacol., 1990, 40(4): 793.
[18] XU Qian-ying, LIU Zhong-fang, HU Xiao-li, et al(许倩影, 刘忠芳, 胡小莉, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2011, 32(7): 1492.
|
[1] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[2] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
[3] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
[4] |
LIN Hong-jian1, ZHAI Juan1*, LAI Wan-chang1, ZENG Chen-hao1, 2, ZHAO Zi-qi1, SHI Jie1, ZHOU Jin-ge1. Determination of Mn, Co, Ni in Ternary Cathode Materials With
Homologous Correction EDXRF Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3436-3444. |
[5] |
LENG Jun-qiang, LAN Xin-yu, JIANG Wen-shuo, XIAO Jia-yue, LIU Tian-xin, LIU Zhen-bo*. Molecular Fluorescent Probe for Detection of Metal Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2002-2011. |
[6] |
QIAN Duo, SU Wen-en, LIU Zhi-yuan, GAO Xiao-yu, YI Yu-xin, HU Cong-cong, LIU Bin, YANG Sheng-yuan*. Soy Protein Gold Nanocluster as an “Off-On” Fluorescent Probe for the Detection of Bacillus Anthracis Biomarkers DPA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1815-1820. |
[7] |
ZHENG Li-zhen1, 2, CHENG Cong2, MA Wen-hua2, WANG Zhuo-rui2, HU Dao-dao2*. Online Detection of Water Forms and Moisture Volatilization Behavior in Earthen Relics Based on FE Fluorescence Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 383-388. |
[8] |
LÜ Chun-qiu1, SI Lu-lu1, PAN Zhao-jin2, LIANG Yang-lin1, LIAO Xiu-fen2, CHEN Cong-jin2*. Fast and Ratiometric Detection of Dimethoate Via the Dual- Emission Center Nitrogen-Doped Carbon Quantum Dots[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 468-474. |
[9] |
SONG Jiang-tao, YUAN Yue-hua, ZHU Yong-jun, WANG Yu-zhen, TIAN Mao-zhong*, FENG Feng*. Research Progress of Near-Infrared Fluorescent Probes for Hydrogen Sulfide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3321-3329. |
[10] |
XU Yi-fei, LIU Lu, SHI Shi-kao*, WANG Yue, PAN Yu-jing, MA Xing-wei. Spectroscopic Properties of Carbon Quantum Dots Prepared From Persimmon Leaves and Fluorescent Probe to Fe3+ Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2418-2422. |
[11] |
DING Jun-nan, WANG Hui, YU Shao-peng*. Application of Rapid Fluorescence Analysis Technology on Study on
Glycine Soja Response to PAHs(Phenanthrene)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2207-2212. |
[12] |
PAN Qiu-li1, SHAO Jin-fa1, LI Rong-wu2, CHENG Lin1*, WANG Rong1. Non-Destructive Analysis of Red and Green Porcelain in Qing Dynasty[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 732-736. |
[13] |
WAN Hao-yu1, ZHOU Zi-xiong1, WU Jun-biao1, Jörg Matysik2, WANG Xiao-jie1*. Spectroscopic Techniques in the Study of Electron Transfer in Flavin Systems[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 368-375. |
[14] |
WAN Xiao-ming1, 2, ZENG Wei-bin1, 2, LEI Mei1, 2, CHEN Tong-bin1, 2. Micro-Distribution of Elements and Speciation of Arsenic in the Sporangium of Pteris Vittata[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 478-482. |
[15] |
ZHU Yu-xuan1,2, LU Jing-bin1, ZHAO Xiao-fan2, LIU Xiao-yan4, CUI Wei-wei2, LI Wei2, WANG Yu-sa2, LÜ Zhong-hua2, 3, CHEN Yong2*. An Application of Lucy Richardson Iterative in X-Ray Fluorescence Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2823-2828. |
|
|
|
|