| 光谱学与光谱分析 |
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| Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoｘyribonucleic Acid and Application to DNA Assay |
| SU Jie-shu1，2，CHEN Xiao-ming1，LONG Yun-fei2，LI Wei2，LUO Hai-tang2 |
1. Institute of Chemistry, Xiangtan University, Xiangtan 411105，China
2. Department of Chemistry, Hu'nan University of Science and Technology, Xiangtan 411201, China |
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Abstract The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid(DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg·L-1 with a correlation coefficient of 0.997 8. The detection limit is 21.5 μg·L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory result.
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Received: 2003-07-16
Accepted: 2003-10-16
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Corresponding Authors:
CHEN Xiao-ming
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| Cite this article: |
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SU Jie-shu,CHEN Xiao-ming,LONG Yun-fei, et al. Resonance Light Scattering Study on the Interaction of Victory Blue-B with Deoｘyribonucleic Acid and Application to DNA Assay [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 37-40.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I01/37 |
[1] LIU Shao-pu, LONG Xiu-fen(刘绍璞, 龙秀芬). Physical Testing and Analysis Part B: Chemical Analysis(理化检验-化学分析), 2002，38(2)：101.
[2] Pasternack R F, Bustanante C, Collings P J et al. J. Am. Chem. Soc., 1993, 115: 5393.
[3] Huang Chengzhi, Li Kean, Tong Shenyang. Anal. Chem.，1996, 68(13): 2259.
[4] Huang Chengzhi, Li Kean, Tong Shenyang. Anal. Chem.，1997, 69(3): 514.
[5] Huang Chengzhi, Li Yuanfang, Liu Xidong. Anal. Chim. Acta, 1998, 375: 89.
[6] WU Hui-ling，LI Wen-you，HE Xi-wen et al(吴会灵, 李文友, 何锡文等). Journal of Analytical Science(分析科学学报)，2002，18(2)：94.
[7] LIU Chen et al(刘 晨等). Chinese Journal of Anal. Chem.(分析化学)，2002，30(10)：1218.
[8] LIU Chen, CHEN Xiao-ming(刘 晨, 陈小明). Chinese Journal of Anal. Chem.(分析化学)，2001，29(6)：685.
[9] LIU Chen，CHEN Xiao-ming，XIANG Hai-yan et al(刘 晨, 陈小明, 向海艳等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(5): 697.
[10] XIANG Hai-yan，CHEN Xiao-ming(向海艳，陈小明). Chinese Journal of Anal. Chem.(分析化学)，2000，28(11)：1398.
[11] XIANG Hai-yan，CHEN Xiao-ming，LI Song-ｑing et al(向海艳，陈小明，李松青等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(6): 822.
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