| 光谱学与光谱分析 |
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| Spectrophotometric Determination of Chondroitin Sulfate with Victory Blue B |
| CHEN Yuan-yuan,JIANG Zhi-liang*,LI Zhen-zhong,ZHOU Su-mei,SUN Shuang-jiao |
| Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China |
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Abstract In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0.1-5 μg·mL-1, and the correlation efficient is 0.998 6. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.
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Received: 2005-02-26
Accepted: 2005-06-18
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Corresponding Authors:
JIANG Zhi-liang
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| Cite this article: |
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CHEN Yuan-yuan,JIANG Zhi-liang,LI Zhen-zhong, et al. Spectrophotometric Determination of Chondroitin Sulfate with Victory Blue B [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(06): 1148-1150.
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https://www.gpxygpfx.com/EN/Y2006/V26/I06/1148 |
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