光谱学与光谱分析 |
|
|
|
|
|
Study on the Chemical Reaction of DNA with Congo Red |
CHI Yan-hua1, ZHUANG Jia1, XUE Qi-bin1, LI Ke-an2 |
1. Department of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621002, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
|
|
Abstract The interaction of deoxyribonucleic acid(DNA) and Congo red (GGH) was investigated by UV-Vis spectrophotometry in Tris-HCl solution (pH 4.56). When DNA was added into GGH solution, the color of the system changed from red to purple, which indicated the formation of the DNA-GGH complex. The maximum absorption of the complex is at 600 nm. The molar absorptivity measured at this wavelength ε=1.41×105 L·cm-1·mol-1, the maximum binding number is n=32,and the detection limit is c=8.04×10-8mol·L-1. The basic reaction condition of best pH value, time, and temperature, and the interference of different materials on the system were also studied. The ionic strength could affect the absorption of the system. The interaction of small molecule and DNA, the molecule structure, and the relationship between the molecule conformation and the distribution of electron cloud were studied.
|
Received: 2004-10-06
Accepted: 2005-01-08
|
|
Corresponding Authors:
CHI Yan-hua
|
|
Cite this article: |
CHI Yan-hua,ZHUANG Jia,XUE Qi-bin, et al. Study on the Chemical Reaction of DNA with Congo Red[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 97-101.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I01/97 |
[1] Szehtki J. Cyclodextrin Technology, Buclapest, Akademial Keidel, 1988. 157. [2] Deratart A, et al. Chem. Phys., 1995, 196: 343. [3] FENG Xi-zeng, XING Wan-li, et al(冯喜增, 邢婉丽, 等). Chem. J. Chinese Universities(高等化学学报),1997,18(10): 1637. [4] Gold D V, Shochat D. Anal. Biochem., 1980, 105: 121. [5] Peters D L, Dahumus M E. Anal. Biochem., 1979, 93: 306. [6] Killeen A A. Microchem. J., 1995, 52: 333. [7] HUANG Hai-zhen,YANG Xiu-rong(黄海珍, 扬秀荣). Chinese Journal of Analytical Chemistry(分析化学), 2002,30:491. [8] CHI Yan-hua, DONG Fa-qin, ZHUANG Jia, ZHAO Zhi-long, LI Ke-an(迟燕华, 董发勤, 庄 稼, 赵志龙, 李克安). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(6): 814. [9] LI Tian-jian, SHEN Han-xi, LUO Yun-jing(李天剑, 沈含熙, 罗云敬). Chinese Journal of Analytical Chemistry(分析化学), 1998, 26: 1372. [10] SHEN Jing-shan, SUN Dan-dan, FU Lian-chun, et al(沈景山, 孙丹丹, 付连春, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(2): 232. [11] ZHANG Li-jin, MIN Shun-geng, LI Guo-xue, et al(张立金, 闵顺耕, 李国学, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(5): 739.
|
[1] |
WU Xiao-yan, ZHAO Lan, LI Lin-lin, LI Jian, WANG Han-yuan, WU Jia*. Characterization of the Effect of Anion on the Complex of Oat β-Glucan and Congo Red with UV-Vis Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(11): 3597-3601. |
[2] |
LU Kui1,2, CHENG Hong-li1, MA Li1, ZHAO Dong-xin1, YANG Liang-ru1, ZHAO Yu-fen3 . Study on the Interaction of L-Cysteine Dipeptide with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 146-149. |
[3] |
ZHANG Guo-wen,QUE Qing-min,PAN Jun-hui. Study on the Recognition between Alpinetin and Calf Thymus DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(08): 1910-1913. |
[4] |
DU feng-pei1,LIU Gang1,LUO Xiao-lin1,HOU Shi-cong1,LI Ke-an2,REN Li-ping,HUANG Qin1,MAO Zhao-shu1,JIE Nian-qin1*. Study on Determination of Acetamiprid and Interaction between Acetamiprid and Deoxyribonucleic Acid by Resonance Light Scattering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1368-1371. |
[5] |
SI Wen-hui1,2,ZI Yan-qing3,TU Yi-feng1*. Spectrophotometric Determination of Deoxyribonucleic Acid by Its Quenching Effect for Acridine Orange[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 412-414. |
[6] |
HE Hua1,WANG Ling-li1,LIU Xu-hui2,JIAO Qing-cai3,CHUONG Pham-Huy4,WANG Guang-ji1* . Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1516-1519. |
[7] |
ZHANG Hai-rong1, 2,GUO Si-yuan2,LI Lin2,CAI Miao-yan2 . Study on the Interaction Mechanism of Targeting Molecules Carboxymethyl Polysaccharide with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 711-714. |
[8] |
TANG Yu-long, GUO Zhou-yi*. Comparison of Influences of Different Intervals of Ultraviolet Irradiation on Calf Thymus Studied by Raman Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 653-657. |
[9] |
BIAN Wei1, WEI Yu-xia2, WEI Yan-li2, DONG Chuan2 . Study on the Interaction of Tetrabromofluorescein and DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 125-129. |
[10] |
WANG Xing-ming1,DONG Fa-qing1,DING Li-sheng2,YANG Ding-ming1,SHI Rong-ming1 . Study on the Reaction of Proteinase from Bacillus Subtilis with Alizarin Complex [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1471-1474. |
[11] |
ZHENG Yu-ying, CAI Wei-long, FU Ming-lian, SUN Rui-qing. Structural Characterization of Rare-Earth Stearate and It’s Thermal Stability Mechanism for PVC [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1533-1536. |
[12] |
RAO Zhen-hong, XIONG Yan-mei, REN Li-ping*, JIANG Shu-ren, ZHANG Chun-rong . Determination of Deoxyribonuceic Acids by Resonance Light Scattering Technique with Benzidine in the Presence of the Sensitizing Sodium Dodecyl Sulfate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1035-1038. |
[13] |
BAI Jin-wei1, CHI Yan-hua1*, ZHUANG Jia1, LI Ke-an2 . Study on the Reaction of Lys-ZCN-CTMAB Complex [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1082-1085. |
[14] |
FENG Su-ling, LIU Xue-ping,FAN Jing . Determination of Deoxyribonucleic Acids Based on the Enhanced Effect of Resonance Light Scattering of Malachite Green Sensitized by Cetyltrimethylammonium Bromide [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 606-609. |
[15] |
LONG Yun-fei1,2,CHEN Xiao-ming1*,CAI Tie-jun2 . Determination of Deoxyribonucleic Acid with Nitro-Tetrazolium Chloride Blue by Resonance Light-Scattering Technique [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 610-612. |
|
|
|
|