| 光谱学与光谱分析 |
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| Study on the Interaction of L-Cysteine Dipeptide with DNA |
| LU Kui1，2, CHENG Hong-li1, MA Li1, ZHAO Dong-xin1, YANG Liang-ru1, ZHAO Yu-fen3 |
1.School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China
2.Henan Institute of Engineering, Zhengzhou 451191, China
3.The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China |
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Abstract In the present paper the interaction mechanism and mode of biologically active cysteine dipeptide (Cys-Cys) with DNA was studied by UV-Vis spectrophotometry and fluorescence spectroscopy using ethidium bromide as a fluorescence probe.The results showed that in the buffer solution of Tris-HCl (pH 7.20), at low concentration of Cys-Cys, the ultraviolet spectrum of DNA-Cys-Cys system produced hypochromic effect with increasing the concentration of Cys-Cys.When the concentration of Cys-Cys increased to some high extent, the ultraviolet spectrum of DNA-Cys-Cys system produced hyperchromic effect.Salt-effect experiment showed that the interaction is liable to be affected by the ionic strengths, suggesting the existence of electrostatic binding between Cys-Cys and DNA.The fluorescence of EB-DNA had quenching effect with increasing the concentration of Cys-Cys, and the Stern-Volmer equation indicated that the quenching process was a static one.According to the Lineweaver-Burk equation the binding constant was determined to be 1.640×104 L·mol-1.From the above results it can be concluded that the interaction mode of Cys-Cys with DNA was mainly electrostatic binding.These findings could contribute to further investigation on the mechanism of oligopeptides interaction with DNA.
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Received: 2009-01-29
Accepted: 2009-05-06
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Corresponding Authors:
LU Kui
E-mail: luckyluke@haut.edu.cn
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