| 光谱学与光谱分析 |
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| Determination of Deoxyribonuceic Acids by Resonance Light Scattering Technique with Benzidine in the Presence of the Sensitizing Sodium Dodecyl Sulfate |
| RAO Zhen-hong, XIONG Yan-mei, REN Li-ping*, JIANG Shu-ren, ZHANG Chun-rong |
| College of Science, China Agricultural University, Beijing 100094, China |
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Abstract The resonance light scattering(RLS) spectra of benzidine with deoxyribonucleic acid(ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS- benzidine system was enhanced by trace ctDNA and was proportional to the concentration of ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg·L-1, the linear regression equation was I=37.17c(ctDNA，mg·L-1 ) +208.9, r=0.999 2. The limit of detection were 0.17 mg·L-1(3δ), RSD%=3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.
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Received: 2003-08-26
Accepted: 2003-12-18
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Corresponding Authors:
REN Li-ping
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| Cite this article: |
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RAO Zhen-hong,XIONG Yan-mei,REN Li-ping, et al. Determination of Deoxyribonuceic Acids by Resonance Light Scattering Technique with Benzidine in the Presence of the Sensitizing Sodium Dodecyl Sulfate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1035-1038.
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http://www.gpxygpfx.com/EN/Y2004/V24/I09/1035 |
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