| 光谱学与光谱分析 |
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| Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe |
| LIU Xue-ping,FENG Su-ling*, PAN Zi-hong, FAN Jing |
| College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China |
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Abstract The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris-HCl buffer solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the obtained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the major controlling factor. The binding constant was found to be 1.0×105 L·mol-1.
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Received: 2005-05-07
Accepted: 2005-10-29
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Corresponding Authors:
FENG Su-ling
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| Cite this article: |
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LIU Xue-ping,FENG Su-ling,PAN Zi-hong, et al. Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1895-1898.
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https://www.gpxygpfx.com/EN/Y2006/V26/I10/1895 |
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