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| Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan |
| SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2* |
1. Research Center of Environmental Technology in Pollution Source Identification and Precise Supervision, School of Environment, Tsinghua University, Beijing 100084, China
2. Research and Development Center of Advanced Environmental Supervision Technology and Instrument, Research Institute for Environmental Innovation (Suzhou), Tsinghua, Suzhou 215163, China
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Abstract In recent years, the fluorescence excitation-emission matrix (EEM) technique has become common for chemical analysis, but fluorescent organic matters with similar structures may exhibit extremely similar EEMs, which may mislead the analysis results. Thus, precisely distinguishing the organic matters with similar EEMs is an important problem to solve. Fluorescence quantum yield (FQY) and fluorescence lifetime (FL) are two important optical parameters for EEM, which are more sensitive to the difference in molecular structure. This study investigated the EEM, FQY, and FL of indole, 3-methylindole, and L-tryptophan. The result showed that their EEMs all displayed one emission (Em) maxima corresponding to two excitation (Ex) maxima, and the position is very close. The fluorescence peaks of indole and L-tryptophan were roughly located at Ex/Em=[275, 340～350] and [220, 340～350] nm, the fluorescence peaks of 3-methylindole were roughly located at Ex/Em=[280, 365] and [225, 365] nm. At the same concentration, these three compounds' maximum fluorescence intensity (MFI) at Ex = 275~280 nm followed this sequence: indole>3-methylindole>L-tryptophan. The integral sphere technique evaluated the FQYs of indole, 3-methylindole and L-tryptophan as 0.264, 0.347, and 0.145, respectively. The FLs of indole, 3-methylindole and L-tryptophan were determined as 4.149, 7.896 and 2.715 ns, respectively. This study indicated that the FQY and FL can distinguish fluorescent organic matters with similar EEMs. The results are of great value in accurately identifying fluorescent organic matters.
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Received: 2022-05-08
Accepted: 2022-10-25
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Corresponding Authors:
WU Jing
E-mail: wu_jing@tsinghua.edu.cn
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[1] Carstea E M, Bridgeman J, Baker A, et al. Water Research, 2016, 95: 205.
[2] Shen J, Liu B, Wu J, et al. Chemosphere, 2020, 239: 124703.
[3] Popik A Y, Gamayunov E L. Pacific Science Review A: Natural Science and Engineering, 2015, 17(1): 29.
[4] Bui M V, Rahman M M, Nakazawa N, et al. Optics Express, 2018, 26(18): 22954.
[5] Cheng C, Wu J, You L D, et al. Chemical Engineering Journal, 2018, 335: 13.
[6] LÜ Qing, GU Jun-qiang, XU Shi-qin, et al（吕 清，顾俊强，徐诗琴，等）. Environmental Monitoring in China（中国环境监测）, 2015, 31(1): 152.
[7] XU Jin-gou, WANG Zun-ben（许金钩，王尊本）. Fluorimetry(荧光分析法). Beijing: Science Press(北京：科学出版社), 2006.
[8] WU Wei, FU Yu, REN Jun-li, et al（吴 伟，傅 宇，任俊丽，等）. Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2020, 40(10): 3108.
[9] Prlj A, Došlic N, Corminboeuf C. Physical Chemistry Chemical Physics: PCCP, 2016, 18: 11606.
[10] Lakowicz J R. Principles of Fluorescence Spectroscopy. Kluwer Academic/Plenum Publishers, New York, 1999.
[11] Ghosh M, Nath S, Hajra A, et al. Journal of Luminescence, 2013, 141: 87.
[12] ZHANG Gui-lan, DONG Xing-fa, CHEN Wen-ju（张桂兰，董兴法，陈文驹）. Chinese Journal of Luminescence(发光学报)，1992，(2): 159.
|
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