| 光谱学与光谱分析 |
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| Study on the Interaction Mechanism of Targeting Molecules Carboxymethyl Polysaccharide with DNA |
| ZHANG Hai-rong1, 2,GUO Si-yuan2,LI Lin2,CAI Miao-yan2 |
1. Laboratory of Biochemical Analysis, Xinzhou Teacher’s University, Xinzhou 034000, China
2. College of Light Industry and Food Science，South China University of Technology，Guangzhou 510640, China |
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Abstract The interaction of chitosan(CTS)，carboxymethyl chitosans(CMCTS), and carboxymethyl cellulose(CMCL) with DNA was investigated through ethidium bromide(EB) as a sensitive fluorescence probe, and ultraviolet absorption spectra. The constant D can directly denote the degree of interaction of polysaccharide with DNA.The results indicated that the interraction sequence of biological polysaccharides with DNA is as follows: CTS＞CMCTS＞CMCL. The polysaccharides were mainly bound to DNA by intercalated binding, which resulted in fluorescence quenching effect of EB-DNA.
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Received: 2004-11-16
Accepted: 2005-04-06
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Corresponding Authors:
CAI Miao-yan2
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| Cite this article: |
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ZHANG Hai-rong,GUO Si-yuan,LI Lin, et al. Study on the Interaction Mechanism of Targeting Molecules Carboxymethyl Polysaccharide with DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 711-714.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I04/711 |
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