甲苯胺蓝共振光散射法测定纳克级脱氧核糖核酸

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摘要：研究了吩噻嗪类染料甲苯胺蓝(TB)与DNA作用的共振光散射光谱，在pH 10～11的范围内，加入DNA导致甲苯胺蓝共振光散射增强，在350 nm处，存在一共振光散射增强峰，其强度与DNA浓度呈线性关系，据此建立了一种测定DNA的共振光散射法。对于ctDNA，方法的线性范围为0～900 ng·mL^-1，检出限为6.75 ng·mL^-1，RSD为3.7％;对于fsDNA，方法的线性范围为0～900 ng·mL^-1, 检出限为2.99 ng·mL^-1，RSD为5.6％．已用于合成样品中DNA的测定。

关键词：甲苯胺蓝;共振光散射法;脱氧核糖核酸

Abstract：Resonance light scattering(RLS) spectra of toluidine blue(TB) with DNA was studied. The RLS of TB was greatly enhanced by DNA in the range of pH 10-11. A RLS peak at 350 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-900 ng·mL^-1 with the detection limit of 6.75 ng· mL^-1 for the ctDNA ,and for fsDNA the linear range was 0-900 ng·mL^-1 with the detection limit of 2.99 ng·mL^-1. Precision at 500 ng·mL^-1 for the two nucleic acids was 3.7% and 5.6%, respectively. Four synthetic samples were determined satisfactorily.

Key words：Toluidine blue;Resonance light-scattering;Calf thymus DNA;Fish sperm DNA

收稿日期: 2003-01-13 修订日期: 2003-06-16

中图分类号:

O657.32

通讯作者: 王永生

引用本文:

肖锡林，王永生^*，李贵荣，吕昌银 . 甲苯胺蓝共振光散射法测定纳克级脱氧核糖核酸[J]. 光谱学与光谱分析, 2004, 24(02): 190-193.
XIAO Xi-lin，WANG Yong-sheng^*，LI Gui-rong，Lü Chang-yin . Determination of Deoxyribonucleic Acids at Nanograms Levels with Toluidine Blue by a Resonance Light-Scattering Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 190-193.

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http://www.gpxygpfx.com/CN/Y2004/V24/I02/190