Determination of Deoxyribonucleic Acids Based on the Enhanced Effect of Resonance Light Scattering of Malachite Green Sensitized by Cetyltrimethylammonium Bromide
FENG Su-ling, LIU Xue-ping，FAN Jing
Key Laboratory of Environmental Science and Engineering Education Commission of He’nan Province, Department of Chemistry, He’nan Normal University, Xinxiang 453002，China
Abstract：A resonance light scattering(RLS) enhancement method was reported for the determination of deoxyribonucleic acids. The proposed method was based on the enhanced effect of deoxyribonucleic acid on the resonance light scattering of malachite green(MG) sensitized by cetyltrimethylammonium bromide(CTMAB). Alkaline medium was necessary for the experiment. Under optimal conditions, the linear ranges for both calf thymus DNA(ctDNA) and fish sperm DNA(fsDNA) were 0-1.5 μg·mL-1, the detection limits were 0.05 μg·mL-1 for ctDNA and 0.03 μg·mL-1for fsDNA. The measurement can be made on normal spectrofluorimeter. Mechanic studies show that MG probably aggregates on the molecular surface of single-chained DNA, and then interacts on CTMAB to form large particles, which produce strong RLS. This method was successfully applied to the determination of DNA in synthetic samples.
冯素玲，刘雪平，樊 静 . 十六烷基三甲基溴化铵敏化孔雀石绿-脱氧核糖核酸的共振光散射增强法测定脱氧核糖核酸 [J]. 光谱学与光谱分析, 2004, 24(05): 606-609.
FENG Su-ling, LIU Xue-ping，FAN Jing . Determination of Deoxyribonucleic Acids Based on the Enhanced Effect of Resonance Light Scattering of Malachite Green Sensitized by Cetyltrimethylammonium Bromide . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 606-609.
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