Study on Determination of Acetamiprid and Interaction between Acetamiprid and Deoxyribonucleic Acid by Resonance Light Scattering
DU feng-pei1,LIU Gang1,LUO Xiao-lin1,HOU Shi-cong1,LI Ke-an2,REN Li-ping,HUANG Qin1,MAO Zhao-shu1,JIE Nian-qin1*
1. College of Science，China Agricultural University, Beijing 100094, China 2. College of Chemistry and Molecular Engineering，Peking University, Beijing 100081, China
Abstract：The interaction between acetamiprid and deoxyribonucleic acid (DNA) was used to determine acetamiprid by resonance light scattering (RLS). The RLS signals of DNA were greatly enhanced by acetamiprid in the spectrum region of 300-600 nm. The spectrum peak is around 316.0 nm. The optimum conditions: pH is 1.73;the concentration of DNA is 2.0 μg·mL-1;the optimum addition order of reagent is acetamiprid -DNA-H2SO4;the reaction time is 30 min;and the linear range of the calibration curve is 0-2.25 μg·mL-1, with the detection limit of 0.2 μg·mL-1. The acetamiprid in river water sample was determined. The results were satisfactory, and the recovery rates were in the range of 98%-106%. The interaction mechanism of acetamiprid and DNA was discussed: the interactions between acetamiprid and nucleic acid base include electrostatic effect and π—π cumulate effect.
杜凤沛1,刘刚1,罗小林1,侯士聪1,李克安2,任丽萍1,黄琴1,毛朝姝1,揭念芹1*. 以光散射技术研究啶虫脒的测定及其与DNA的相互作用[J]. 光谱学与光谱分析, 2008, 28(06): 1368-1371.
DU feng-pei1,LIU Gang1,LUO Xiao-lin1,HOU Shi-cong1,LI Ke-an2,REN Li-ping,HUANG Qin1,MAO Zhao-shu1,JIE Nian-qin1*. Study on Determination of Acetamiprid and Interaction between Acetamiprid and Deoxyribonucleic Acid by Resonance Light Scattering. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1368-1371.
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