Study on the Interaction of L-Cysteine Dipeptide with DNA
LU Kui1,2, CHENG Hong-li1, MA Li1, ZHAO Dong-xin1, YANG Liang-ru1, ZHAO Yu-fen3
1.School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China 2.Henan Institute of Engineering, Zhengzhou 451191, China 3.The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract:In the present paper the interaction mechanism and mode of biologically active cysteine dipeptide (Cys-Cys) with DNA was studied by UV-Vis spectrophotometry and fluorescence spectroscopy using ethidium bromide as a fluorescence probe.The results showed that in the buffer solution of Tris-HCl (pH 7.20), at low concentration of Cys-Cys, the ultraviolet spectrum of DNA-Cys-Cys system produced hypochromic effect with increasing the concentration of Cys-Cys.When the concentration of Cys-Cys increased to some high extent, the ultraviolet spectrum of DNA-Cys-Cys system produced hyperchromic effect.Salt-effect experiment showed that the interaction is liable to be affected by the ionic strengths, suggesting the existence of electrostatic binding between Cys-Cys and DNA.The fluorescence of EB-DNA had quenching effect with increasing the concentration of Cys-Cys, and the Stern-Volmer equation indicated that the quenching process was a static one.According to the Lineweaver-Burk equation the binding constant was determined to be 1.640×104 L·mol-1.From the above results it can be concluded that the interaction mode of Cys-Cys with DNA was mainly electrostatic binding.These findings could contribute to further investigation on the mechanism of oligopeptides interaction with DNA.
卢 奎1,2,成红丽1,马 丽1,赵东欣1,杨亮茹1,赵玉芬3 . L-半胱氨酸二肽与DNA的相互作用研究[J]. 光谱学与光谱分析, 2010, 30(01): 146-149.
LU Kui1,2, CHENG Hong-li1, MA Li1, ZHAO Dong-xin1, YANG Liang-ru1, ZHAO Yu-fen3 . Study on the Interaction of L-Cysteine Dipeptide with DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 146-149.
[1] LI Hua, YU Hu-xin, YAN Ying, et al(李 华, 余胡昕, 燕 影, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(8): 1905. [2] LIU Guang-dong, SONG Gong-wu, CAI Zhao-xia(刘光东, 宋功武, 蔡朝霞).Journal of Instrumental Analysis(分析测试学报), 2004, 23(5): 21. [3] WAN Hong-yan, ZHANG Ya-feng, CHEN Jing-hua, et al(万红艳, 张亚锋, 陈敬华, 等).Journal of Instrumental Analysis(分析测试学报), 2007, 26(1): 59. [4] LI Hong, ZHAO Wen-jie, LU Kui(李 红, 赵文杰, 卢 奎).Chemical Journal of Chinese Universities(高等学校化学学报), 2004, 25(10): 1866. [5] Clareand D A, Swaisgood H E.J.Dairy.Sci., 2000, 82(1): 1187. [6] LI Rui, LU Kui, MA Li, et al(李 锐, 卢 奎, 马 丽, 等).Chinese Journal of Analysis Laboratory(分析实验室), 2007, 26(9): 21. [7] WU Rui-guang, WEI Zhi-wu, CHEN Lin, et al(邬瑞光,尉志武,陈 琳,等).Chemical Journal of Chinese Universities(高等学校化学学报), 2002, 23: 2366. [8] JIN Lan, YANG Pin, LI Qing-shan(靳 兰,杨 频,李青山).Chemical Journal of Chinese Universities(高等学校化学学报), 1996, 17(9): 1345. [9] PENG Mao, CHEN Juan, SONG Gong-wu(彭 毛, 陈 娟, 宋功武).Journal of Analytical Science(分析科学学报), 2008, 24(2): 153. [10] YANG Gong-jun, XU Jing-juan, CHEN Hong-yuan(杨功俊,徐静娟,陈洪渊).Chemical Journal of Chinese Universities(高等学校化学学报),2004,25(7): 1239. [11] Gao E J, Zhu M C, Yin H X.Journal of Inorganic Biochemistry, 2008, 102: 1958. [12] CHENG Jian-hua, LIU Miao, ZHOU Xi-liu, et al(程建华, 刘 淼, 周西柳, 等).Journal of Jilin Universities·Science Ed.(吉林大学学报·理科版), 2007, 45(3): 486. [13] Scatchard G.Annals of the New York Aacademy of Sciences, 1949, 51(4): 660. [14] Ware W R.Journal of Physical Chemistry, 1962, 66: 455. [15] WANG Li, ZHANG Ai-ping, YANG Jin-yan, et al(王 丽, 张爱萍, 杨锦艳, 等).Chinese Remedies and Clinics(中国药物与临床), 2007, 7(9): 667. [16] YANG Man-man, YANG Pin, ZHANG Li-wei(杨曼曼, 杨 频, 张立伟).Chinese Science Bulletin(科学通报), 1994, 39(1): 31. [17] MEI Lin, CHENG Zheng-xue, SHI Kai-yun(梅 林,程正学,石开云).Laser Journal(激光杂志),2007,28(3): 84.