Investigation of Exogenous Stimulus Effect on the Interaction of CdSe/ZnS Quantum Dots/TiO2 Nanocomposites with Human Serum Albumin by Resonance Light Scattering
LI Yue-sheng, DU Ji-fu, SUN Shao-fa, ZHAO Long*
Non-Power Nuclear Technology Collaborative Innovation Center, Hubei University of Science & Technology, Xianning 437100, China
Abstract：The interaction between CdSe/ZnS(quantum dots)/TiO2 nanocomposites and human serum albumin(HSA) was investigated by resonance light scattering (RLS) spectroscopic methods under approximate physiological conditions. Much important information of the interaction between CdSe/ZnS(Quantum Dots)/TiO2 nanocomposites and HSA was obtained by studying comprehensively the Exogenous influence factors of nanocomposites concentration, pH, NaCl concentration, reaction temperature, detection time, coexisting ions, surfactants, sequence of adding to the sample etc. It was showed that the new formation of complex system is likely to enhance the protein hydrophobic cavity and tend to focus the hydrophobic interface in aqueous solution, resulting in strengthening the intensity of resonance light scattering; Also it is very sensitive to the changes in the pH value in the system; The sensitivity of IRLS in system can be increased by the appropriate NaCl concentration; The value of IRLS in system would be changed with the change in the concentration of coexisting ions; The value of IRLS in system is basically stable when the reaction time reaches 5 min; The value of IRLS in system is not exactly the same with a surfactant, and strong electrostatic interaction has occurred between oppositely charged surfactant and nano composites; It is obvious that the value of IRLS in complex system is affected by the sequence of adding to sample; It has the incomplete monotonically increasing trend with the changes in temperature. The information is useful for providing theoretical supporting for the mechanism of interaction between nanomaterials and bio-macromolecule, and for understanding the biocompatibility and safety of nano-materials.
李月生，杜纪富，孙绍发，赵 龙* . 共振光散射法(RLS)研究外源刺激对CdSe/ZnS量子点/TiO2纳米复合物与人血清白蛋白的相互作用的影响 [J]. 光谱学与光谱分析, 2014, 34(04): 1040-1044.
LI Yue-sheng, DU Ji-fu, SUN Shao-fa, ZHAO Long* . Investigation of Exogenous Stimulus Effect on the Interaction of CdSe/ZnS Quantum Dots/TiO2 Nanocomposites with Human Serum Albumin by Resonance Light Scattering. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 1040-1044.
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