Abstract:The recognition between alpinetin and DNA under physiological condition (pH 7.4) was investigated by fluorescence and UV-visible spectrometry.The experiment demonstrated that the fluorescence of alpinetin could be quenched by DNA.A quenching mechanism was proved to be the single static quenching procedure according to the deescalating quenching constants KSV (3.288×103, 2.923×103 and 2.467×103 L·mol-1, respectively) along with the escalating temperatures (25, 32 and 39 ℃) and the quenching rate constant Kq was greater than the maximum scatter collision quenching rate constant of various quenchers with the biomolecule.From the UV-visible spectra of alpinetin and DNA, the result showed that the UV-visible spectra of alpinetin did not changed in the presence of DNA, i.e.neither the decrease in the maximum absorbance intensity nor the red shift of the maximum absorption wavelength changed.Both the fluorescence intensity and the maximum emission wavelength of ethidium bromide-DNA system remained unchanged in the presence of alpinetin, indicating that there is no direct competition for binding DNA between alpinetin and ethidium bromide.Furthermore, DNA thermal denaturation test indicated that the fluorescence quenching effect of alpinetin with unlinking DNA was stronger than that of alpinetin with natural DNA.It was concluded that there was not intercalation binding mode between alpinetin and DNA.At the same time, fluorescence quenching effect and salt effect on the binding of alpinetin with DNA were investigated.It was shown that the major mode of recognition was groove binding between alpinetin and DNA.
张国文,阙青民,潘军辉. 山姜素与脱氧核糖核酸的相互识别研究[J]. 光谱学与光谱分析, 2008, 28(08): 1910-1913.
ZHANG Guo-wen,QUE Qing-min,PAN Jun-hui. Study on the Recognition between Alpinetin and Calf Thymus DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(08): 1910-1913.
[1] LIU Jun, LUO Guo-an, WANG Yi-ming, et al(刘 军, 罗国安, 王义明, 等).Acta Pharm.Sin.(药学学报), 2001, 36(1):74. [2] CAO Ying, HE Xi-wen(曹 瑛,何锡文).Journal of Analytical Science(分析科学学报), 1998, 14(3):244. [3] Cody V, Middleton E, Harborne J B.Plant Flavonoids in Biology and Medicine:Biockemical, Phomacological and Stracture Activity Relationships.New York:Alan R.Liss, 1986. [4] Bi S Y, Qiao C Y, Song D Q, et al.Sensors and Actuators B, 2006, 119:199. [5] HE Ji-xiang, JIANG Chong-qiu, GAO Ming-xia(贺吉香, 江崇球, 高明霞).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4):755. [6] Sun Hongxia, Tang Yalin, Xiang Junfeng, et al.Bioorganic & Medicinal Chemistry Letters, 2006, 16(13):3586. [7] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al (陈国珍, 黄贤智, 郑朱梓, 等).The Methods of Fluorescence Analysis(Second ed) (荧光分析法, 第2版).Beijing:Science Press(北京:科学出版社), 1990. [8] Lakowicz J R.Principles of Fluorescence Spectroscopy.New York:Plenum Press, 1983, 257. [9] LI Gai-xian, LI Jian-qing, WEI Yu-xia, et al(李改仙, 李建晴, 魏玉霞, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8):1277. [10] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容, 郭祀远, 李 琳, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6):829. [11] YAN Cheng-nong, ZHANG Hua-xin, LIU Yi, et al(颜承农, 张华新, 刘 义, 等).Acta Chimica Sinica(化学学报), 2005, 63(18):1727. [12] Ni Y N, Lin D Q, Kokot S.Analytical Biochemistry, 2006, 352(2):231. [13] Hopkins H P, Yang M, Wilson W D, et al.Biopolymers, 1991, 31:1105. [14] Lepecq J B, Paoletti C.Journal of Molecular Biology, 1967, 27:87. [15] Kumar C V, Asuncion E H J.Journal of the Chemical Society-Chemical Communication, 1992, (6):470. [16] Kumar C V, Tumer R S, Asuncion E H.J.Photochem.Photobiol A:Chem., 1993, 74(2-3):231. [17] LI Lai-sheng, HUANG Zhi-bing, WANG Yu-xiao, et al(李来生, 黄志兵, 王宇晓, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(7):1088. [18] Pasternack R F, Brigandi R A, Abrams M J.Inorg.Chem., 1990, 29:4483. [19] MEI Wen-jie, LI An-xing(梅文杰, 李安兴).Chemical Research in Chinese Universities(高等学校化学学报), 2002, 23(11):2049.