Study on the Spectrophotometric Determination of Hydroxyl Free Radical from Low Power Trench-Type Ultrasound
CAO Yan-ping1，2, YUAN Ying-mao1，2, ZHU Yu-chen1，2
1. School of Food & Chemical Engineering, Beijing Technology & Business University, Beijing 100048, China 2. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048, China
Abstract：Under the condition of different pH (7~11) and different ethanol volume fraction (45% to 85%), the ultraviolet-visible absorption spectra of malachite green were studied in neutral and alkaline ethanol solution, the maximum absorption wavelength at 620 nm was found, and the matching degree of standard curve was better established. In low power trench-type ultrasound apparatus, the absorption of the malachite green solution was measured under ultrasound and non-ultrasound, respectively. the difference values of the ultraviolet absorption of the malachite green solution under low power trench-type ultrasound were measured results of the hydroxyl free radical oxidation degrading malachite green, therefore hydroxyl free radical from low power trench-type ultrasound was determined indirectly. Then the contents of hydroxyl free radical in four conditions were measured. The detection limit of the method of 8.4×10-6 mmol·L-1 and the relative standard deviation of the method of 9.4×10-5～3.7×10-4 mmol·L-1 were determined, a higher testing precision and good reproducibility were confirmed. It can be applied for fast detection of neutral and alkaline ethanol solution system in the case of very low concentration of hydroxyl free radicals. Since malachite green is heat sensitive, so compared to measuring temperature, the method possessed better functions for thermal effects of ultrasound.
曹雁平1，2，袁英髦1，2，朱雨辰1，2 . 低强度超声场中羟自由基分光光度法检测研究[J]. 光谱学与光谱分析, 2012, 32(05): 1320-1323.
CAO Yan-ping1，2, YUAN Ying-mao1，2, ZHU Yu-chen1，2 . Study on the Spectrophotometric Determination of Hydroxyl Free Radical from Low Power Trench-Type Ultrasound . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(05): 1320-1323.
[1] Suslick K S, Didenko Y, Fang M, et al. Philosophical Transactions A, 1999, 357(1751): 335. [2] Price G J, Duck F A, Digby M, et al. Ultrasonics Sonochemistry, 1997, 4(2): 165. [3] Kaur H, Halliwell B. Analytical Biochemistry, 1994, 220(1): 11. [4] Lin S H, Lo C C. Water Research, 1997, 31(8): 2050. [5] MA Qian，SUN Li-hong(马 茜, 孙丽红). Chinese Journal of Analysis Laboratory(分析试验室), 2006, 25(12): 87. [6] WU Nan, REN Feng-lian, WU Xin-chuan(吴 南, 任凤莲, 吴心传). Journal of Instrumental Analysis(分析测试学报), 2001, 20(2): 52. [7] QIN Yan-he(秦延河). Journal of Jiangxi Normal University·Natural Science Edition(江西师范大学学报·自然科学版), 2005, 29(6): 519. [8] ZHANG Ai-mei, LI Lin-wei, HAO Gui-qi, et al(张爱梅, 李林尉, 郝贵奇, 等). Journal of Instrumental Analysis(分析测试学报), 2005, 24(4): 83. [9] Zhu B Z, Zhao H T，Kalyanaraman B, et al. Free Radical Biology and Medicine, 2001, 32(5): 465. [10] Steiner M G, Babbs C F. Archives of Biochemistry and Biophysics, 1990, 278(2): 478. [11] Berberidou C, Poulios I, Xekoukoulotakis N P, et al. Applied Catalysis B: Environmental, 2007, 74(1-2): 63. [12] YUAN Ying-mao，CAO Yan-ping(袁英髦, 曹雁平). Science and Technology of Food Industry(食品工业科技), 2011, 32(3): 442. [13] Koda S, Kimura T, Kondo T, et al. Ultrasonics Sonochemistry, 2003, 10(3): 149. [14] DENG Bo(邓 勃). Statistical Analysis of Test Data Processing Method(分析测试数据的统计处理方法). Beijing：Tsinghua University Press(北京: 清华大学出版社), 1995. 291.
