Study on the Interaction of Tetrabromofluorescein and DNA
BIAN Wei1, WEI Yu-xia2, WEI Yan-li2, DONG Chuan2
1. Basic Medical College of Shanxi Medical University, Taiyuan 030001, China 2. Shanxi University, Institute of Chemistry and Chemical Engineering, Taiyuan 030006, China
Abstract：The interaction of tetrabromofluorescein (TBF) and DNA was studied by the fluorescence and SS-RTP. The effect of the ion strength and ethanol on the fluorescence spectra of TBF in the presence of DNA and absence of DNA was investigated. In addition, the effect of pH on the SS-RTP spectra of TBF was also examined. The quenched constant was 719.74 and 880.22 in the absence and presence of DNA respectively. Finally, the interaction mode between TBF and DNA was discussed from the fluorescence quenched and phosphorescence polarized experiments.
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