Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry
LIU Yu1, LI Zeng-wei2, DENG Zhi-peng1, ZHANG Qing-xian1*, ZOU Li-kou2*
1. Key Laboratory of Geosciences and Nuclear Technology,Chengdu University of Technology,Chengdu 610059,China
2. College of Resources, Sichuan Agricultural University, Chengdu 611130, China
Abstract:In recent years, food safety accidents by microbial contamination is a considerable threat to health. The rapid detection of microorganisms is of great significance to food safety. At present, the rapid microorganism detection technology is a difficult operation and high cost. Laser-induced fluorescence spectrometry (LIFS), with the advantages of high sensitivity, convenient operation, relatively cheap equipment, could provide a potential technique for rapid detection of microorganisms. In this paper, we use a portable 405 nm laserto excitefluorescence of three common foodborne pathogenic bacteria (Enterococcus faecalis, Salmonella Typhimurium and Pseudomonas aeruginosa), and a fiber spectrometerto detect the spectra. By adjusting the laser power (10~100 mW) to get the fluorescence intensity of Enterococcus faecalis, the relationship between the laser power and bacterial fluorescence intensity has been verified, and the result showed the optimal laser power range of 50 to 80 mW. In this experiment, the fluorescence spectra between bacterial samples are obtained at P=50 mW. We discussed the relationship between bacterial structure and fluorescence spectra. According to the research result, It was concluded that E. faecalis exhibited a fluorescence peak of flavonoid groups near 528 nm, and the fluorescence peak at 634 nm of P. aeruginosa corresponds to the fluorescence emission of protoporphyrin. The results showed that, (1) the fluorescence peaks at 634 and 703 nm of P. aeruginosa for excitation are different from other two bacteria, which can be a feature for direct detection; (2) based on statistics, the spectrum of E. faecalis and S. Typhimurium was divided into 9 characteristic areas, and the recognition rate of the two bacteria reached 100% detected by the dynamic clustering method. The results show that the laser-induced fluorescence spectrometry can effectively detect P. aeruginosa, E. faecalis and S. Typhimurium. Compared with other rapid detection techniques, LIFS has significant application value for the rapid detection of foodborne pathogenic bacteria with the easier operation, faster detection speed and higher recognition rate.
刘 宇,李增威,邓志鹏,张庆贤,邹立扣. 激光诱导荧光光谱快速检测食源性致病菌[J]. 光谱学与光谱分析, 2021, 41(09): 2817-2822.
LIU Yu, LI Zeng-wei, DENG Zhi-peng, ZHANG Qing-xian, ZOU Li-kou. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2817-2822.
[1] Kim Y, Jett J H, Larson E J, et al. Cytometry, 1999, 36(4):324.
[2] GAO Wen-xuan, GAN Zhi-lin, CHEN Ai-liang, et al(高雯暄, 甘芝霖, 陈爱亮, 等). Journal of Food Safety & Quality(食品安全质量检测学报), 2020, 11(24): 9440.
[3] Shelly D C, Quarles J M, Warner I M. Clinical Chemistry, 1980, 26(8):1127.
[4] Héctor Enrique Giana, Landulfo Silveira, Renato Amaro Zangaro, et al. Journal of Fluorescence, 2003, 13(6):489.
[5] Arabi D S, Abdel-Salam Z A, Goda H A, et al. Journal of Luminescence, 2018, 194:594.
[6] Hemant Bhatta, Ewa M Goldys, Robert P Learmonth. Applied Microbiology and Biotechnology, 2006, 71(1):121.
[7] Jeanne L McHale. Molecular Spectroscopy(分子光谱). Beijing: Science Press(北京:科学出版社),2003. 151.
[8] Koenig K, Schneckenburger H. Journal of Fluorescence, 1994, 4(1): 17.
[9] ZHANG Ling-ling, CHEN Yuan, WANG Cai-xia, et al(张玲玲,陈 媛,王彩霞,等). Applied Laser(应用激光), 2019, 39(6): 1035.
[10] WANG Jing-yan, ZHU Sheng-geng, XU Chang-fa, et al(王镜岩,朱圣庚,徐长法,等). Biochemistry(生物化学). Beijing: Higher Education Press(北京:高等教育出版社),2002. 51.
[11] YAO Meng, WANG Hai-shui(姚 蒙,王海水). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2020, 40(Suppl.): 27.