Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1
1. Institute for Quality & Safety and Standards of Agricultural Products Research, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
3. Laboratory of Quality & Safety Risk Assessment for Animal Products (Nanchang), Ministry of Agriculture and Rural Affairs, Nanchang 330200, China
Abstract：A method of ion-pair reversed phased liquid chromatography-ultraviolet (IP-RP-HPLC)-hydride generation-atomic fluorescence spectrometry (AFS) was established to determine the contents of selenocystine, methylselenocysteine, selenotmethionine, selenateand selenite in livestock and poultry meat. Organic Se species contents in samples were extracted by trypsin and protease (XIV, pronase), and inorganic Se species contents in samples were extracted with iodine acetamide solution and water incubated at 55 ℃ with shaking at 200 r·min-1for 20 h. The extraction solution of samples was centrifuged at high speedand then centrifuged with microsep for purification. C18 separated the solution of samples reversed phase column by using 30 mmol·L-1diammonium hydrogen phosphate, 0.5 mmol·L-1tetrabutyl-ammonium bromide and 5% (V/V) methanol as moblie phase. The pH of the mobile phase was adjusted to 6.0 by 20% (V/V) formic acid. IP-RP-HPLC-AFS determined the contents of five Se species in samples solution. The impurities are qualitatively determined by contrasting with the standard sample and quantitatively determined by calculating the peak areas. Selenocystine, methylselenocysteine, selenotmethionine, selenate and selenite had good linearities in the range of 5~200 μg·L-1, and the correlation coefficients were all greater than 0.999, the detection limits of five Se species were 0.89, 0.78, 0.55, 0.94 and 0.70 μg·L-1, respectively. There coveries were between 76.8%~109%, the within-run precisions and between-run precisions were 2.7%~6.8% and 3.5%~12.3% respectively. The proposed method has the advantages of rapid, simple, high sensitivity and high accuracy, and it is suitable for Se species analysis in livestock and poultry meat samples.
Key words：IP-RP-HPLC; AFS; Livestock and poultry meat; Se species
魏益华，黄青青，张金艳，邱素艳，涂田华，袁林峰，戴廷灿，张标金，李伟红，严 寒. 离子对反相液相色谱-原子荧光光谱法测定畜禽肉中5种硒形态含量[J]. 光谱学与光谱分析, 2021, 41(12): 3822-3827.
WEI Yi-hua, HUANG Qing-qing, ZHANG Jin-yan, QIU Su-yan, TU Tian-hua, YUAN Lin-feng, DAI Ting-can, ZHANG Biao-jin, LI Wei-hong, YAN Han. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827.
