Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry
ZHAO Ting1,2,3, CHI Hai-tao1,2,3*, LIU Yi-ren1,2,3, GAO Xia1,2,3, HUANG Zhao1,2,3, ZHANG Mei1,2,3, LI Qin-mei1,2,3
1. Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China
2. Beijing Key Laboratory of Organic Materials Testing Technology ＆ Quality Evaluation, Beijing 100089, China
3. Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China
Abstract：X-ray fluorescence spectrum micro-area analysis method has the characteristics of rapid, simple and non-destructive testing, and can detect the distribution of elements on the surface of health food. Inductively coupled plasma mass spectrometry has the advantages of low detection limit, wide linear range and multi-element simultaneous determination. Methods: A health food was analyzed by X-ray fluorescence spectrum micro-region analysis technique, and the elemental content was semi-quantitatively analyzed to determine the elements of calcium (Ca), iron (Fe), ruthenium (Ru) and molybdenum (Mo) in health products. The main elements of Ca and Fe were quantitatively analyzed by microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS). The average value of Ca element was 6.23%, the relative standard deviation was 1.78%, the average value of the Fe element was 3.82%, and the relative standard deviation was 2.14%. The results were consistent with the conclusion that the content of Ca in the sample with X-ray fluorescence spectrum micro-analysis was between 6.0% and 10.0%, and the content of Fe in the sample was between 2.0% and 4.0%. The distribution of the elements on the surface of health food was not uniform by X-ray fluorescence spectrum analysis. The X-ray fluorescence spectrum micro-area analysis method cannot only measure the elemental semi-quantitative content in health food by using the trace sample, but also measure the elemental distribution in health food. Combined with inductively coupled plasma mass spectrometry, the content of the elements of interest in the sample can be further detected, and the quantitative analysis results can be obtained.
Key words：X-ray fluorescence spectrometry (XRF); X-ray fluorescence spectrum microregion; Inductively coupled plasma mass spectrometry (ICP-MS); Health food
赵 婷，池海涛，刘奕忍，高 峡，黄 瞾，张 梅，李琴梅. X射线荧光微区分析结合电感耦合等离子体质谱法进行保健食品中元素含量的测定[J]. 光谱学与光谱分析, 2021, 41(03): 750-754.
ZHAO Ting, CHI Hai-tao, LIU Yi-ren, GAO Xia, HUANG Zhao, ZHANG Mei, LI Qin-mei. Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 750-754.
[1] DAI Xiang-dong, XU Yang-biao, LIU Zhuang-wei, et al（戴向东, 许杨彪, 刘庄蔚, 等）. Chinese Journal of Information on TCM(中国中医药信息杂志), 2013, 20(1): 106.
[2] WANG Hua-kun, CHEN Hai-bo, YIN Li(王华坤, 陈海博, 尹 丽). Capital Medicine(首都医药), 2013, (4): 15.
[3] ZHANG Jian，WANG Zhen-xu，LI Xiu-mei(张 剑, 王祯旭, 李秀梅). China Pharmaceuticals(中国药业), 2016, 25(5): 52.
[4] CHEN Zhe(陈 喆). Strait Pharmaceutical Journal(海峡药学), 2016, 28(11): 52.
[5] RAN Hua-kang, ZHANG Fu-jun, YIN Xiao-ling（冉华康，张伏军，尹小玲）. Chinese Pharmacist（中国药师）, 2009, 12(10): 1487.
[6] ZAN Ya-ling, TENG Hong-mei, WANG Lei（昝亚玲, 滕红梅, 王 磊）. Journal of Anhui Agricultural Science（安徽农业科学）, 2015, 43(36): 218.
[7] LI Jian, ZHANG Man, JIANG Yu-jun（李 健，张 曼，姜毓君）. China Dairy Industry（中国乳品工业）, 2016, 44(10): 52.
[8] Sayo O Fakayode，Owen S L, Pollard D A. American Journal of Analytical Chemistry, 2013, 4(7): 348.
[9] Gao Jingjing, Liu Jihua, Li Xianguo, et al. Acta Oceanol. Sin., 2017, 36(1): 109.
[10] Jin Pengfei, Liang Xiaoli, Xia Lufeng, et al. Journal of Trace Elements in Medicine and Biology, 2016, 33: 73.
[11] Franky Puype, Ji( rˇ )í Samsonek, Jan Knoop, et al. Food Additives & Contaminants: Part A, 2015, 32(3): 410.
[12] XU Cong, ZHAO Ting, CHI Hai-tao, et al(徐 聪, 赵 婷, 池海涛, 等). China Measurement & Test(中国测试), 2019, 45(5): 85.
[13] ZHANG Cai-xia, SUN Zhong, YE Jian-sheng(张彩霞, 孙 忠, 叶建圣). Rock and Mineral Analysis(岩矿测试), 2012, 31(2): 272.
[14] LIANG Shu-ting, LIU Yu-chun, LIU Zhen, et al（梁述廷, 刘玉纯, 刘 瑱, 等）. Rock and Mineral Analysis（岩矿测试）, 2013, 32(6): 897.
[15] ZHAO Ting, ZHOU Ming-qiang, XU Cong, et al（赵 婷, 周明强, 徐 聪, 等）. Journal of Food Safety & Quality（食品安全质量检测学报）, 2016, 7(11): 4352.
[16] LIN Hao, ZHANG Yun-ping（林 昊, 张云平）. Medical Front（医药前沿）, 2017, 7(12): 178.
[17] HU Yi-chun, CHEN Jing, LI Min, et al（胡贻椿, 陈 竞, 李 敏, 等）. Chin. J. Prev. Med.（中华预防医学杂志）, 2016, 50(3): 213.
