Fluorescence Characteristic of Terbium-Ciprofloxacin Complex and Its Application
HE Hua1,WANG Ling-li1,LIU Xu-hui2,JIAO Qing-cai3,CHUONG Pham-Huy4,WANG Guang-ji1*
1. China Pharmaceutical University，Nanjing 210009, China 2. INSERM U-602，Institut du Cancer et d’Immunogénétique (ICIG)，Hopital Paul Brousse，94807，Villejuif，France 3. Pharmaceutic Biotechnology Key Laboratory，College of Life Science，Nanjing University，Nanjing 210093，China 4. Faculty of Pharmacy, University of Paris V, 4 Avenue de l’Observatoire, 75006, Paris, France
Abstract：On binding to deoxyribonucleic acid, the complex of terbium-ciprofloxacin (Tb3+-CIP) increases its fluorescence quantum efficiency. Based on this, an easy, rapid and sensitive method for the determination of DNA was developed. Like ethidium bromide (EB), the complex can be intercalated into DNA bases, but it is non-poisonous. Determination can be made at pH 7.0, where the native structure of DNA is not disrupted. The maximum emission is at 545 nm with excitation at 325 nm. This method has good sensitivity (2.8×10-9 mol·L-1 of ctDNA), high selectivity and a wide linear range (4..3×10-7-3.0×10-5 mol·L-1 of ctDNA). This complex was also employed for the clinical examination of DNA in whole blood of tumor patients. Ten tumor patients and 10 normal persons were enrolled in this study. It was showed that significant difference existed between control group and tumor group (p＜0.05).
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