A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3
1. College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, China
2. Key Laboratory of Cognitive Science, State Ethnic Affairs Commission, Wuhan 430074, China
3. Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis ＆ Treatment, Wuhan 430074, China
Abstract：Carbon Quantum Dots (CQDs), a class of zero-dimensional carbon nanomaterials with significant fluorescence properties, have become popular in biosensing application research in recent years. Lead comes from many sources in environments such as cosmetics and industrial pollution, and inhalation or ingestion of lead adsorbed on particulate matter can cause lead poisoning, which can cause various diseases, so point of care detection of lead ion (Pb2+) content is extremely important in clinical medical applications. Based on the fluorescence characteristics of carbon quantum dots, a new blue and red dual-emission ratio fluorescent probe was proposed for rapid detection of lead ion content, and the morphological structure and properties of the probe were characterized, detected and analyzed by transmission electron microscope, fluorescence spectroscopy and other means, and the optical properties and application feasibility of Pb2+ response probe were studied in depth. The double emission carbon point is calibrated by comparison with itself to improve the detection effect and sensitivity of the analyte concentration, and effectively avoid the interference of the external environment. This proportional fluorescent uses aqueous synthesis, which is simple and reproducible, and can rapidly respond to Pb2+ in just a few seconds. The detection process can be observed naked-eye from blue to red with only an ultraviolet lamp, which can be used for clinical point-of-care detection. In the concentration range of Pb2+ in line with current medical applications of 0~0.5 mg·L-1, the fluorescence intensity IBCDs/IRCDs has a good linear relationship with concentration, R2=0.987 44, and the detection limit is 0.013 6 mg·L-1. The progressive fluorescence sensing of the probe with Zn2+, Fe3+, K+ and other ten metal interfering ions showed that the probe had a good specific selection for Pb2+. The lead response was measured at different pH environments and incubation times to investigate probe stability.
Key words：Carbon quantum dots; Ratio fluorescent probe; Fluorescence quenching; Immediate detection; Lead ions
易敏娜，曹汇敏，黎双娜丝，张朱珊莹，朱春楠. 用于快速检测铅离子的新型双发射碳点比率荧光探针[J]. 光谱学与光谱分析, 2023, 43(12): 3788-3793.
YI Min-na, CAO Hui-min, LI Shuang-na-si, ZHANG Zhu-shan-ying, ZHU Chun-nan. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793.
[1] Ratnarathorn N, Chailapakul O, Dungchai W, et al. Talanta, 2015, 132: 613.
[2] Yuan Zhiqin, Peng Meihua, He Yan, et al. Chemical Communications, 2011, 47: 11981.
[3] Wang Yuedan, Zhou Zhou, Qing Xing, et al. Analytical and Bioanalytical Chemistry, 2016, 408(21): 5779.
[4] Fatima Ezzahra Salih, Aicha Ouarzane, Mama El Rhazi, et al. Arabian Journal of Chemistry, 2017, 10(5): 596.
[5] Thirumalai M, Kumar S N, Prabhakaran D, et al. Journal of Chromatography A, 2018, 1569: 62.
[6] Duan Hualing, Zhang Ningning, Gong Zhenbin, et al. Spectrochimica Acta Part B: Atomic Spectroscopy, 2016, 120: 63.
[7] Zhao Qin, Rong Xiaolong, Chen Li，et al. Talanta, 2013, 114: 110.
[8] Nerea De Acha, César Elosúa, Jesús M Corres, et al. Sensors, 2019, 19(3): 599.
[9] Zhang Xuejie, Wang Xiangtao, Qiu Hanhong, et al. Colloids and Surfaces B: Biointerfaces, 2020, 189: 110876.
[10] Vinayak Sahu, Fahmida Khan. Heliyon, 2020, 6(5): e03957.
[11] Wang Chuanxi, Wu Jiapeng, Jiang Kaili, et al. Sensors and Actuators B: Chemical, 2017, 238: 1136.
[12] He Guili, Shu Mengjun, Yang Zhi, et al. Applied Surface Science, 2017, 422: 257.
[13] Zubair M S H Khan, Raja Saifu Rahman, Shumaila, et al. Optical Materials, 2019, 91: 386.
[14] Sajad Moradi, Komail Sadrjavadi, Negin Farhadianb, et al. Journal of Molecular Liquids, 2018, 259: 284.
[15] Pramod Kumar Mehta, Jong yong Jeon, Ki Ryu, et al. Journal of Hazardous Materials, 2022, 427: 128161.
[16] Gao Zhikun, Hao Tongfan, Fang Qunxiang, et al. Methods and Applications in Fluorescence, 2021, 9(1): 015004.
[17] SHI Ji-yong, LI Wen-ting, HU Xue-tao, et al(石吉勇, 李文亭, 胡雪桃, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(12): 3925.
[18] Shu Yun, Dai Tao, Ye Qiuyu, et al. Journal of Fluorescence, 2021, 31(6): 1947.
[19] LIAN Jie, REN Yi-fei, YANG Rui-qin, et al(廉 洁, 任翼飞, 杨瑞琴, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2020, 40(3): 804.
[20] Li Wenting, Hu Xuetao, Li Qian, et al. Food Chemistry, 2020, 320: 126623.
[21] Wang Haiqian, Yang Liang, Chu Suyun, et al. Analytical Chemistry, 2019, 91(14): 9292.
[22] Lu Hongzhi, Yu Chunwei, Xu Shoufang. Sensors and Actuators B: Chemical, 2019, 288: 691.
[23] Yeji Kim, Jongsung Kim. Optical Materials, 2020, 99: 109514.
[24] Xavier S S J, Siva G, Annarajb J, et al. Sensors and Actuators B: Chemical, 2018, 259: 1133.
