甲苯胺蓝荧光猝灭法测定核酸

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摘要：以甲苯胺蓝为荧光探针，基于DNA对甲苯胺蓝的荧光猝灭作用，建立了一种定量测定DNA的新方法。在pH 8.5的Tris-HCl缓冲溶液中，测定小牛胸腺DNA的线性范围为0.1～6.0 μg·mL^-1，检测限为27 ng·mL^-1。该方法应用于实际样品樟树嫩叶中的DNA含量的测定，获得了满意结果。

关键词：核酸;甲苯胺蓝;荧光探针

Abstract：A new fluorimetric method has been developed for the determination of deoxyribonucleic acid (DNA) with toluidine blue(TB) as a fluorescence probe. It is based on the fluorescence quenching of toluidine blue in the presence of DNA. In Tris-HCl buffer solution (pH 8.5), the calibration graph was linear over the range 0.10-6.00 μg·mL^-1 for ctDNA, and the detection limit was 27 ng·mL^-1. The result of the determination of DNA of camphor tree’s leaf by this method was satisfactory.

Key words：Nucleic acids;Toluidine blue;Fluorescence probe

收稿日期: 2004-06-26 修订日期: 2004-11-15

中图分类号:

O657.3

通讯作者: 谢增鸿

引用本文:

郭良洽，叶芳贵，林旭聪，谢增鸿^*. 甲苯胺蓝荧光猝灭法测定核酸[J]. 光谱学与光谱分析, 2006, 26(01): 121-124.
GUO Liang-qia,YE Fang-gui,LIN Xu-cong,XIE Zeng-hong^*. Determination of Nucleic Acids Using Toluidine Blue as a Fluorescence Probe. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 121-124.

链接本文:

https://www.gpxygpfx.com/CN/Y2006/V26/I01/121

[1] CHEN Fang，ZHOU Fei，HE Zhi-ke(陈芳，周飞，何治柯). Chinese Journal of Analytical Chemistry(分析化学), 2003，31(12): 1450.
[2] WANG Jing-wu，ZHU Xia-ping，SUN Wei, XUE Jian-guo, WANG Zhao-xi, et al(汪敬武，朱霞萍，孙韡, 薛建国, 王兆喜，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003，23(5): 899.
[3] LI Wen-you，WU Hui-ling，HE Xi-wen, et al(李文友，吴会灵，何锡文). Chemical Journal of Chinese Universities(高等学校化学学报), 2003，24(10): 1787.
[4] Prat O，Lopez E，Mathis G. Analytical Biochemistry，1991，195: 283.
[5] HUANG Cheng-zhi，LI Ke-an，TONG Shen-yang(黄承志，李克安，童沈阳). Chinese Journal of Analytical Chemistry(分析化学), 1997, 25(7): 768.
[6] Ci Yunxiang, Li Yuanzong, Liu Xiaojing. Analytical Chemistry, 1995, 67(11): 1785.
[7] WANG Xing-min，SONG Yu-min，TENG Xiu-lan, GENG Zhi-yuan, YANG Pei-ju, et al(王兴民，宋玉民，滕秀兰, 耿志远, 杨培菊，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(3): 339.
[8] LUO Li，JIANG Chong-qiu，LI Lei(罗黎，江崇球，李磊). Chinese Journal of Analytical Chemistry(分析化学), 2003，31(12): 1504.
[9] ZHU Zhan-cai，WANG Jing，ZHU Hong-bin(朱展才，汪静，朱红斌). Chinese Journal of Analytical Chemistry(分析化学), 2002，30(11): 1319.
[10] GAO Feng，ZHU Chang-qing，WANG Lun(高峰，朱昌青，王伦). Spectroscopy and Spectral Analyris(光谱学与光谱分析), 2004，24(1): 85.
[11] WANG Le-yu，GUO Chang，LI Mao-guo, et al(汪乐余，郭畅，李茂国, 等). Chinese Journal of Analytical Chemistry(分析化学), 2003，31(1): 83.
[12] XIAO Xi-lin，WANG Yong-sheng，LI Gui-rong, Lü Chang-yin(肖锡林，王永生，李贵荣, 吕昌银). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004，24(2): 190.
[13] Killeen Anthony A. Microchemical Journal, 1995，52(3): 333.