Study on the Chemical Reaction of DNA with Congo Red
CHI Yan-hua1, ZHUANG Jia1, XUE Qi-bin1, LI Ke-an2
1. Department of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621002, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Abstract：The interaction of deoxyribonucleic acid(DNA) and Congo red (GGH) was investigated by UV-Vis spectrophotometry in Tris-HCl solution (pH 4.56). When DNA was added into GGH solution, the color of the system changed from red to purple, which indicated the formation of the DNA-GGH complex. The maximum absorption of the complex is at 600 nm. The molar absorptivity measured at this wavelength ε=1.41×105 L·cm-1·mol-1, the maximum binding number is n=32，and the detection limit is c＝8.04×10-8mol·L-1. The basic reaction condition of best pH value, time, and temperature, and the interference of different materials on the system were also studied. The ionic strength could affect the absorption of the system. The interaction of small molecule and DNA, the molecule structure, and the relationship between the molecule conformation and the distribution of electron cloud were studied.
迟燕华1，庄 稼1，薛其彬1，李克安2 . 脱氧核糖核酸与刚果红化学反应的机理研究[J]. 光谱学与光谱分析, 2006, 26(01): 97-101.
CHI Yan-hua1, ZHUANG Jia1, XUE Qi-bin1, LI Ke-an2 . Study on the Chemical Reaction of DNA with Congo Red. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 97-101.
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