1. Laboratory of Biochemical Analysis, Xinzhou Teacher’s University, Xinzhou 034000, China 2. College of Light Industry and Food Science，South China University of Technology，Guangzhou 510640, China
Abstract：The interaction of chitosan(CTS)，carboxymethyl chitosans(CMCTS), and carboxymethyl cellulose(CMCL) with DNA was investigated through ethidium bromide(EB) as a sensitive fluorescence probe, and ultraviolet absorption spectra. The constant D can directly denote the degree of interaction of polysaccharide with DNA.The results indicated that the interraction sequence of biological polysaccharides with DNA is as follows: CTS＞CMCTS＞CMCL. The polysaccharides were mainly bound to DNA by intercalated binding, which resulted in fluorescence quenching effect of EB-DNA.
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