1. College of Chemistry and Environment Science, Hebei University, Baoding 071002, China 2. Bioinorganic Chemistry Laboratory, Agricultural University of Hebei, Baoding 071001, China
Abstract：The interaction of adriamycin (ADM) and DNA results in strongly enhanced resonance light scattering (RLS) spectra characterized by two peaks at 322 and 564 nm, respectively. The interaction of ADM and DNA was investigated by using absorption spectra, fluorescence spectra, and RLS spectra. The results indicate that RLS spectra have the highest sensitivity. It was found that the enhanced RLS intensity at 322 nm was proportional to the concentration of DNA in the range of 0-8.0 μg·mL-1. An RLS method for the determination of DNA was accordingly described. The limit of determination was 36.8 and 40.1 ng·mL-1 for calf thymus DNA and fish sperm DNA, respectively. The proposed method has a high sensitivity and good selectivity due to the specificity of the interaction of ADM and DNA.
冯 硕1, 2,李正平1*,张淑红2,方 正2 . 阿霉素与DNA作用的共振光散射研究及在DNA分析中的应用[J]. 光谱学与光谱分析, 2006, 26(04): 707-710.
FENG Shuo1，2,LI Zheng-ping1*,ZHANG Shu-hong2,FANG Zheng2 . Study on Interaction of Adriamycin and DNA with a Resonance Light Scattering Technique and Its DNA Analytical Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(04): 707-710.
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