DNA Binding and Antitumor Activity of a New Fluorescent Probe Eu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety
Belal H M Hussein1,2*, Mostafa A Gouda1,3, Omer Sakin1, Abdulla Faluji4, Mohamed Gomaa5, Hassan A Azab2, Walid Fathalla6, Sherin Arabi7, Sawsan Mosa8
1. Chemistry Department, Faculty of Science & Arts, Al Ula, Taibah University, Al Madina Al Monawarah, KSA
2. Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia, Egypt
3. Chemistry Department, Faculty of Science, Mansoura University, Al Mansoura, Egypt
4. Biotechnology Research Center, Suez Canal University, Ismailia 41522, Egypt
5. Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
6. Department of Mathematical and Physical Sciences, Faculty of Engineering, Port-Said University, Port-Said, Egypt
7. Department of Mathematical and Physical Sciences, Faculty of Engineering, Suez Canal University, Ismailia, Egypt
8. Department of Chemistry, Faculty of Education, el Arish University, Egypt
DNA Binding and Antitumor Activity of a New Fluorescent Probe Eu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety
Belal H M Hussein1,2*, Mostafa A Gouda1,3, Omer Sakin1, Abdulla Faluji4, Mohamed Gomaa5, Hassan A Azab2, Walid Fathalla6, Sherin Arabi7, Sawsan Mosa8
1. Chemistry Department, Faculty of Science & Arts, Al Ula, Taibah University, Al Madina Al Monawarah, KSA
2. Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia, Egypt
3. Chemistry Department, Faculty of Science, Mansoura University, Al Mansoura, Egypt
4. Biotechnology Research Center, Suez Canal University, Ismailia 41522, Egypt
5. Medicinal Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
6. Department of Mathematical and Physical Sciences, Faculty of Engineering, Port-Said University, Port-Said, Egypt
7. Department of Mathematical and Physical Sciences, Faculty of Engineering, Suez Canal University, Ismailia, Egypt
8. Department of Chemistry, Faculty of Education, el Arish University, Egypt
摘要： A new europium (Ⅲ) complex containing (4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid moiety (CMMC) was synthesized, characterized, and confirmed as antitumor agent and fluorescent probe. The spectroscopic measurements of Eu(Ⅲ) in the presence of CMMC were obtained in different solvents. The results show that the strongest Eu(Ⅲ) emission bands were monitored in iso-propyl alcohol while the weakest Eu(Ⅲ) emission band was observed in acetonitrile. The interaction of Eu(Ⅲ)-(CMMC)2 complex with DNA was monitored using absorption and emission techniques. From fluorescence titration measurements, the binding constants of DNA with Eu(Ⅲ)-(CMMC)2 complex were found to be 1.04×105 L·mol-1 in Tris-HCl and 1.17×107 L·mol-1 in DMSO-Tris-HCl buffer (9∶1 V/V). Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Eu(Ⅲ)-complex between the base pair of DNA. This result further confirmed by fluorescent Ethidium bromide displacement assay. The fluorescence calibration curve was used for the determination of DNA with LOD of 1.2 ng in DMSO-Tris-HCl buffer (9∶1 V/V) and 5 ng in Tris-HCl buffer. The preliminary antitumor investigation shows promising cytotoxicity against MDA-MB-231, MCF-7 (mammary cancer), and PC-3 (prostate carcinoma) cell lines with IC50 values of 40.63, 25.42 and 30.25 μmol·L-1, respectively.
关键词：Europium；(4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid; DNA sensing; Spectroscopic properties; Antitumor activity
Abstract：A new europium (Ⅲ) complex containing (4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid moiety (CMMC) was synthesized, characterized, and confirmed as antitumor agent and fluorescent probe. The spectroscopic measurements of Eu(Ⅲ) in the presence of CMMC were obtained in different solvents. The results show that the strongest Eu(Ⅲ) emission bands were monitored in iso-propyl alcohol while the weakest Eu(Ⅲ) emission band was observed in acetonitrile. The interaction of Eu(Ⅲ)-(CMMC)2 complex with DNA was monitored using absorption and emission techniques. From fluorescence titration measurements, the binding constants of DNA with Eu(Ⅲ)-(CMMC)2 complex were found to be 1.04×105 L·mol-1 in Tris-HCl and 1.17×107 L·mol-1 in DMSO-Tris-HCl buffer (9∶1 V/V). Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Eu(Ⅲ)-complex between the base pair of DNA. This result further confirmed by fluorescent Ethidium bromide displacement assay. The fluorescence calibration curve was used for the determination of DNA with LOD of 1.2 ng in DMSO-Tris-HCl buffer (9∶1 V/V) and 5 ng in Tris-HCl buffer. The preliminary antitumor investigation shows promising cytotoxicity against MDA-MB-231, MCF-7 (mammary cancer), and PC-3 (prostate carcinoma) cell lines with IC50 values of 40.63, 25.42 and 30.25 μmol·L-1, respectively.
Key words：Europium；(4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid; DNA sensing; Spectroscopic properties; Antitumor activity
基金资助: supported by grants 4590/1434 by Deanship of Scientific Research at Taibah University in Saudi Arabia for financial assistance
通讯作者:
Belal H M Hussein
E-mail: belalhussein102@yahoo.com
引用本文:
Belal H M Hussein, Mostafa A Gouda, Omer Sakin, Abdulla Faluji, Mohamed Gomaa, Hassan A Azab, Walid Fathalla, Sherin Arabi, Sawsan Mosa. DNA Binding and Antitumor Activity of a New Fluorescent Probe Eu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety[J]. 光谱学与光谱分析, 2018, 38(11): 3611-3621.
Belal H M Hussein, Mostafa A Gouda, Omer Sakin, Abdulla Faluji, Mohamed Gomaa, Hassan A Azab, Walid Fathalla, Sherin Arabi, Sawsan Mosa. DNA Binding and Antitumor Activity of a New Fluorescent Probe Eu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3611-3621.
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