Study on FTIR Spectra of Finger Nails of Normal People and Patients of Esophagus Cancer
WANG Hong-yan1,Lü Yin1,WANG Fan1*,MA Xiao-dong2,JIANG Shi-ping2,WANG Wei2,LI Cheng-xiang2
1. Department of Radiation Oncology, Anhui Medical University Hospital, Hefei 230022, China 2. National Synchrotron Radiation Laboratory，University of Science and Technology of China, Hefei 230029, China
Abstract：To investigate the application of human finger nails in the diagnosis of cancer, Fourier transform infrared (FTIR) spectroscopy was employed to study the finger nails from some normal people and some with esophagus cancer and others with an operation for curing esophagus cancer five months ago. The results showed that there are obvious differences between FTIR spectra in them in spectral parameters such as frequency, intensity and band shape etc. The changes in the phosphate symmetric stretching vibration νs(PO-2) and asymmetric stretching vibration νas(PO-2) modes are uniform, the νs(PO-2) and νas(PO-2) absorption peaks of cancerous ones shift to high wave number compared with those of normal people, while those with operation shift to low wave number compared with those of cancerous ones. The C—O stretching vibration mode of protein located at 1 164 cm-1 is composed of three absorption peaks located at 1 173.3，1 158.0 and 1 151.1 cm-1 respectively, meanwhile, the intensities and the wave numbers of the three peaks of cancerous ones all increase compared with normal people. The wave numbers of amide Ⅰ and amide Ⅱ of cancerous ones are both lower than those of normal people, while those with operation are between the cancerous ones and normal people, which suggest that the contents of protein and α-helix in finger nails of normal people, cancerous ones and the ones with operation are discriminative. The peak of bending vibration δ(CH2)mode of CH2 groups of protein lipid of cancerous ones shifts to high wave number slightly and the intensity of the peak weakens compared with that of normal people, which indicate that the methylene chain in the finger nails membrane lipids of cancerous ones is more ordered than that of normal people. Nevertheless, the peak of stretching vibration νs(CH2)of cancerous ones is lower than that of normal people, while that of the ones with operation is between cancerous ones and normal ones. As a result, the pathological changes in viscera can induce the changing of framework structure of phosphate in nucleic acid molecule of finger nails, and destroy the hydrogen bond of C—O(H) group in amino acid remnant group in finger nails. Furthermore, it also can decrease the C—O bond in keratin which participates in the action of hydrogen bond, and prompt the changing of content of keratin in finger nails, nevertheless the content of keratin in finger nails of cancerous ones can be resumed to level of normal people by operation.
汪红艳1,吕银1,王凡1*,马晓冬2,蒋诗平2,王卫2,李承祥2 . 食管癌患者指甲的红外光谱研究[J]. 光谱学与光谱分析, 2008, 28(02): 331-334.
WANG Hong-yan1,Lü Yin1,WANG Fan1*,MA Xiao-dong2,JIANG Shi-ping2,WANG Wei2,LI Cheng-xiang2 . Study on FTIR Spectra of Finger Nails of Normal People and Patients of Esophagus Cancer. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 331-334.
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