Analysis of Spectral Characteristics of Soil Under Different Continuous Cropping of Rehmannia Glutinosa Based on Infrared Spectroscopy
QIAO Lu1, LIU Rui-na1, ZHANG Rui1, ZHAO Bo-yu1, HAN Pan-pan1, 2, ZHOU Chun-ya1, 3, ZHANG Yu-qing1, 4, DONG Cheng-ming1*
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
2. School of Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
3. School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China
4. The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China
Abstract：Continuous cropping obstacle is the greatest difficulty in Rehmannia glutinosa planting. It must be replanted after 8～10 years. However, the mechanism of the continuous cropping obstacles is not clear yet. An analytical method based on Fourier transform infrared spectroscopy (FTIR-ATR) and Two-Dimensional Infrared Correlation Spectroscopy (2D-IR) is proposed. Collected from the main crop (YA-One), second crop (YA-Two), 10-year-old interval (YA-Ten) and blank (CK) of the spectra of four kinds of Rehmannia glutinos soil samples. Norris noise filtering and smoothing，second derivative and two-dimensional correlation spectrum analysis were performed to extract characteristic spectra，analyzed the fingerprint of compounds in the soil. The results showed that the peaks of different continuous cropping soils were similar, and the main characteristic absorption peaks of spectra were around 3 621, 3 439, 2 932, 2 860, 2 513, 1 798, 1 636, 1 433, 1 029, 887, 783, 695, 546 and 469 cm-1. Compared with CK, the absorption intensity of YA-Two is the strongest, and these absorption peaks mostly represent the functional groups such as hydroxy —OH, carbonyl C═O, C—O, and substituted absorption of the benzene ring in phenolic acids, indicating the accumulation of phenolic acids in Rehmannia glutinos in continuous cropping. The reverse stretching vibration and symmetric stretching vibration of saturated methylene CH2 in the glycosides were observed At t 2 925 and 2 857 cm-1. The absorption intensity of Ya-Two was the smallest, and Ya-Ten was the largest. The results indicated that the glucoside components decreased in continuous cropping soil. The position, number and strength of automatic peak and cross peak were different in the two-dimensional infrared correlation spectra of the soil of Rehmannia glutinos in bands 920～1 750, 1 490～1 710 and 1 500～1 548 cm-1, which clearly showed the difference of functional groups. The results showed that the rapid detection of organic compounds in soil could be achieved by using Fourier transform infrared spectroscopy (FTIR) and two-dimensional infrared correlation spectroscopy (TD-IR), which provided a theoretical basis for studying Rehmannia glutinos continuous cropping obstacles.
乔 璐，刘瑞娜，张 瑞，赵博宇，韩盼盼，周春亚，张雨晴，董诚明. 基于红外光谱法对不同连作茬次地黄土壤光谱特征分析[J]. 光谱学与光谱分析, 2023, 43(02): 541-548.
QIAO Lu, LIU Rui-na, ZHANG Rui, ZHAO Bo-yu, HAN Pan-pan, ZHOU Chun-ya, ZHANG Yu-qing, DONG Cheng-ming. Analysis of Spectral Characteristics of Soil Under Different Continuous Cropping of Rehmannia Glutinosa Based on Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 541-548.
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