Abstract:The traditional screening method of cervical cancer mainly involves TBS classification method and quantitative analysis method based on DNA, TBS screening method which has high diagnostic rate, but it needs experienced doctors to participate in the process with low sensitivity. Therefore, it is difficult to achieve early cervical cancer screening; cell’s DNA quantitative analysis, only stained the nucleus, achieving a quantitative and automated analysis. Even with high sensitivity, the specificity is poor. So it is extremely necessary to realize the combination of screening for TBS and cell’s DNA quantitative analysis, but the current TBS and DNA quantitative analysis combined screening method for the use of two different cell smear, time-consuming, laborious and very inconvenient, there is no screening method for cervical cancer on a combination of TBS and DNA quantitative analysis at home and abroad. This paper presents a method using TBS classification and DNA quantitative analysis on the same cell smear which was stained with Pap and Feulgen in order to solve the problem of the interference of the absorbance of DNA substance caused by multiple staining. a set of multi spectral imaging system and DNA absorbance peeling model are established based on linear multiple regression. With model solution, the real absorbance of the substance DNA was calculated, and the quantitative analysis of DNA was carried out. A pseudo color image is synthesized from 3 - band cell images with the close wavelength of RGB for TBS classification, so the organic combination of TBS and cell’s DNA quantitative analysis is realized. Experiments show that the DNA quantitative analysis model of this method is stable, with small error and high diagnostic rate due to the fact that the pseudo color images used for TBS screening were bright, clear, and clear cytoplasm. Therefore, this method is very useful in the diagnosis and screening of cervical cancer.
王佳华,吴 正,吴琼水*,曾立波* . 基于光谱成像技术的宫颈癌TBS与细胞DNA定量分析联合筛查方法研究 [J]. 光谱学与光谱分析, 2017, 37(02): 545-550.
WANG Jia-hua, WU Zheng, WU Qiong-shui*, ZENG Li-bo* . The Study on Combined Screening Method for TBS and Cervical Cancer Cell’s DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 545-550.
[1] ZOU Xue-mei(邹雪梅). Chinese Journal of Disease Control & Prevention(中华疾病控制杂志), 2010, 14(1): 84. [2] ZHAO Tong-xiang,DENG Xiao-hong, HAN Li-li(赵同香, 邓小虹, 韩历丽). Chinese Journal of Disease Control & Prevention(中华疾病控制杂志),2013, 17(8): 715. [3] Cheung F Y, Mang O W, Law S C. Hong Kong Med. J., 2011, 2(17): 89. [4] Whitlock E P, Vesco K K, Eder M, et al. Ann. Intern. Med., 2011, 155(10): 687. [5] Biesterfeld S, Beckers S, Del Carmen Villa Cadenas M, et al. Anticancer Res., 2011, 31(1): 53. [6] Tong H, Shen R, Wang Z, et al. Clin. Cancer. Res., 2009, 15(20): 6438. [7] CHEN Chun-yan, GE Wen-juan(陈春艳, 戈文娟). Guangxi Medical Journal(广西医学), 2013, 35(5): 594. [8] Colditz G A, Crowley J. Clin. Cancer Res., 2011, 17(22): 6971. [9] LU Wan-zhen(陆婉珍). Modern Near Infrared Spectroscopy Analytical Technology(现代近红外光谱分析技术). 2nd Ed.(第2版). Beijing: China Petrochemical Press(北京:中国石化出版社), 2006. 37.