Spectrophotometric Determination of Deoxyribonucleic Acid by Its Quenching Effect for Acridine Orange
SI Wen-hui1,2,ZI Yan-qing3,TU Yi-feng1*
1. College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215123, China 2. Suzhou Agricultural Polytechnic College, Suzhou 215008, China 3. Huaibei Coal Industry Teachers College, Huaibei 235000, China
Abstract：Based on the reaction of acridine orange with deoxyribonucleic acid(DNA), and under the optimized condition, a novel method to determine the DNA was developed with the spectrophotometry. The absorbance of acridine orange at the maximum absorption wavelength of 444 nm responded to the concentration of DNA negatively with excellent linearity. It has an upper linear limit concentration of 8.0 μg·mL-1 and a detection limit of 0.12 μg·mL-1 with the correlation coefficient of 0.999 8. The method is simple, rapid and sensitive, and could be applied to sample assay satisfactorily. Finally, the reaction mechanism is discussed.
Key words：DNA;Acridine orange;Stack up reaction;Determination;Spectrophotometry
司文会1,2,訾言勤3,屠一锋1*. 吖啶黄反应光度法测定脱氧核糖核酸含量的研究[J]. 光谱学与光谱分析, 2008, 28(02): 412-414.
SI Wen-hui1,2,ZI Yan-qing3,TU Yi-feng1*. Spectrophotometric Determination of Deoxyribonucleic Acid by Its Quenching Effect for Acridine Orange. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 412-414.
