1. Medical College of Shandong University, Ji’nan 250012, China 2. The Affiliated First Hospital of Fujian Medical University, Fuzhou 350005, China 3. College of Preclinical Medicine of Fujian Medical University, Fuzhou 350004, China 4. College of Chemistry & Materials, Fujian Normal University, Fuzhou 350007, China 5. State Key Laboratory for Structure Chemistry, Chinese Academy of Sciences, Fuzhou 350002, China
Abstract：The tetra-sulfonated phathalocyanine cobalt(CoPcS4)was synthesized by using 4-sulfonic phthalic acid as raw material, CoCl2 as template agent and (NH4)2MoO4 as catalyst. CoPcS4 was demonstrated to be C4h mono-isomer by elementary analysis, IR, NMR and HLPC. Depolymerization of CoPcS4 was found in DMSO. The depolymerization increased with the pH value raising. The interaction of tetra-sulfonated phathalocyanine cobalt with bovine serum albumin (BSA) was studied by UV-Vis and fluorescence spectra. The number of binding sites and binding constant were determined at equal pace of the order of magnitude 105 L·mol-1. CoPcS4 binds with BSA at SiteⅠ and SiteⅡ. In conclusion, CoPcS4 binds with BSA commendably and albumin plays a role of storage and transport.
Key words：Tetra-sulfonated cobalt phathalocyanine;Bovine serum albumin;Compete-binding;Parameters of binding
黄焱1,3,徐国兴2*,彭亦如4*,翁家宝4,5. 四磺基酞菁钴合成及其与牛血清白蛋白结合作用的研究[J]. 光谱学与光谱分析, 2008, 28(02): 407-411.
HUANG Yan1,3,XU Guo-xing2*,PENG Yi-ru4*,WENG Jia-bao4,5. Study on the Interaction of Tetra-Sulfonated Phathalocyanine Cobalt with Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 407-411.
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