氯化硝基四氮唑蓝共振散射法检测DNA

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摘要：研究了氯化硝基四氮唑蓝与脱氧核糖核酸在酸性条件下的共振光散射特征，考察了影响因素和最佳反应条件，建立了一种测定DNA的新方法。在pH=1.8三羟甲基氨基甲烷(Tris-HCl)缓冲溶液中，线性范围为0～7 mg·L^-1，相关系数为0.995 8，检出限为30.04 ng·mL^-1，用于合成样品的测定结果令人满意。

关键词：氯化硝基四氮唑蓝;脱氧核糖核酸;共振散射光谱

Abstract：Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid(DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated，and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg·L^-1 with a correlation coefficient of 0.995 8. The detection limit is 30.04 μg·L^-1. The method has been applied to the determination of DNA in mixed samples with satisfactory result.

Key words：Nitro-tetrazolium chloride blue;DNA;Resonance scattering spectroscopy

收稿日期: 2002-10-16 修订日期: 2003-01-26

中图分类号:

O644.14

通讯作者: 陈小明

引用本文:

龙云飞^1，2，陈小明^1*，蔡铁军² . 氯化硝基四氮唑蓝共振散射法检测DNA [J]. 光谱学与光谱分析, 2004, 24(05): 610-612.
LONG Yun-fei^1，2，CHEN Xiao-ming^1*，CAI Tie-jun² . Determination of Deoxyribonucleic Acid with Nitro-Tetrazolium Chloride Blue by Resonance Light-Scattering Technique . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 610-612.

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http://www.gpxygpfx.com/CN/Y2004/V24/I05/610