十二烷基硫酸钠敏化联苯胺共振光散射技术测定脱氧核糖核酸

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摘要：研究了在十二烷基硫酸钠作用下联苯胺与小牛胸腺脱氧核糖核酸(ct DNA)作用的共振光散射光谱(RLS)的特征。痕量核酸对联苯胺-十二烷基硫酸钠的RLS有增强作用，且增强的程度与核酸的浓度成线性关系。在优化条件下确定了RLS强度与核酸浓度的线性范围为0.3～ 4 mg·L^-1, 线性方程为I=37.17 c(ctDNA，mg·L^-1)+208.9, 相关系数r=0.999 2。方法的检出限为0.17 mg·L^-1(3δ)，相对标准偏差3.5%。该方法成功地用于人工混合样品和转基因烟草中的DNA含量测定。

关键词：十二烷基硫酸钠;共振光散射;联苯胺;脱氧核糖核酸

Abstract：The resonance light scattering(RLS) spectra of benzidine with deoxyribonucleic acid(ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS- benzidine system was enhanced by trace ctDNA and was proportional to the concentration of ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg·L^-1, the linear regression equation was I=37.17c(ctDNA，mg·L^-1 ) +208.9, r=0.999 2. The limit of detection were 0.17 mg·L^-1(3δ), RSD%=3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.

Key words：Sodium Dodecyl Sulfate;Resonance lights scattering;Benzidine;Deoxyribonuceic acid

收稿日期: 2003-08-26 修订日期: 2003-12-18

中图分类号:

O657.3

通讯作者: 任丽萍

引用本文:

饶震红，熊艳梅，任丽萍^*，江树人，张春荣 . 十二烷基硫酸钠敏化联苯胺共振光散射技术测定脱氧核糖核酸 [J]. 光谱学与光谱分析, 2004, 24(09): 1035-1038.
RAO Zhen-hong, XIONG Yan-mei, REN Li-ping^*, JIANG Shu-ren, ZHANG Chun-rong . Determination of Deoxyribonuceic Acids by Resonance Light Scattering Technique with Benzidine in the Presence of the Sensitizing Sodium Dodecyl Sulfate . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1035-1038.

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