1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China 2. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
Abstract：The characteristics of resonance light scattering (RLS), UV-visible absorption spectra and fluorescence spectra of [Cu(DPPZ)(L-Ser)]+ with DNA were studied and a RLS method for the determination of DNA was established. [Cu(DPPZ)(L-Ser)]+ could assemble on the surface of DNA through intercalation, and enhanced the RLS of DNA in the tris buffer of pH 7.2. The maximum resonance light scattering peak appeared at 400 nm. Under the optimum conditions, the enhanced intensity of RLS was proportional to the concentration of DNA over the range of 0.42～4.2 ng·mL-1, with a detection limit (3σ/k) of 0.29 ng·mL-1. The method was used for the determination of DNA samples with the recoveries between 97.8% and 106%.
Key words：Resonance light scattering;Copper;L-serine;Dipyrido[3;2-a;2′;3′-c]phenazine;Calf thymus DNA
吴呈珂1，董秋鹏1,2，张 红1，熊 岑1，吴秀丽1，凌连生1* . [Cu(DPPZ)(L-Ser)]+与DNA的相互作用及其分析应用研究 [J]. 光谱学与光谱分析, 2011, 31(04): 1057-1060.
WU Cheng-ke1, DONG Qiu-peng1，2, ZHANG Hong1, XIONG Cen1, WU Xiu-li1, LING Lian-sheng1* . Spectral Study on the Interaction of [Cu(DPPZ)(L-Ser)]+ Complex with DNA and Its Analytical Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(04): 1057-1060.
[1] Hunter C A，Sanders J K M. J. Am. Chem. Soc., 1990, 112: 5525. [2] Tamotsu S, Hideki M, Nayumi O. et al. Inorg. Chem., 1997, 36: 576. [3] Debarati D, Adity B, Nikhil P. et al. J. Phys. Chem. A，2008, 112: 3943. [4] Gao E，Shi Q，Liu L，et al. Chin. J. Chem., 2009, 27(12)：2341. [5] ZHANG Zhi-jun, LI Xi, HAO Li, et al(张志军，李 曦，郝 莉，等). Journal of Huazhong Normal University(华中师范大学学报·自然科学版)，2008，42(3): 409. [6] GU Qin, REN Xiang-xiang, LE Xue-yi(古 琴, 任祥祥, 乐学义). Acta Physico-Chimica Sinica(物理化学学报)，2008, 24(6): 1068. [7] Maheswari P U, Roy S, Dulk H, et al. J. Am. Chem. Soc., 2006, 128(3): 710. [8] Li J, Kang J, Lu J，et al. Journal of Luminescence，2009, 129: 906. [9] Long Y F, Huang C Z, Li Y F. J. Phys. Chem. B，2007, 111: 4535. [10] FENG Su-ling, LIU Xue-ping, FAN Jing(冯素玲，刘雪平，樊 静). Chinese Journal of Analytical Chemistry(分析化学)，2005, 33: 377. [11] Zou Q，Yan Q, Song G，et al. Biosens. Bioelectron., 2007, 22: 1461. [12] He W, Huang C Z, Li Y F，et al. Anal. Chem., 2008, 80: 8424. [13] Liu Z, Li Y F, Ling J，et al. Environ. Sci. Tech., 2009, 43: 5022. [14] Wang K, Qiu X, Dong C，et al. Chem-Bio-Chem., 2007, 8: 1126. [15] Du B, Li Z, Liu C. Angew. Chem. Int. Ed., 2006, 45: 8022. [16] Wolfe A, Shimer Jr G H, Meehan T. Biochem., 1987, 26(20): 6392. [17] Lakowicz J R, Weber G. Biochem., 1973, 12(21): 4161.
