Abstract：The effects of concentration and type of anions on aggregation of Congo red in solution were studied with UV-Visible spectroscopy. And the influence of anions on the complex of Congo red and oat β-glucan was further studied. The results showed that the maximum absorption wavelength of Congo red in aqueous solution shifted toward the shorter wavelength, and the absorbency lowered considerably with increasing ionic concentration. There was a significant linear correlation between logarithm of anion concentration and the maximum absorption wavelength, with the peak absorbance, and the absorbance at 499 nm. The influence of anions on aggregation of Congo red was in accordance with Hofmeister series, indicating that hydrophobic interaction is an important driving force for Congo red molecules to assemble into micelles. As for the complexation of Congo red and oat β-glucan, when the concentration of anions exceeded the first critical concentration, the micelle of Congo red began to form and then combined to β-glucan. The absorption peak of the complex presented at 556 nm. When the concentration of anions exceeded the second critical concentration, the complex was developed into supramolecular structure through aggregation of Congo red micelles on Congo red/β-glucan complex, which resulted in the shift of absorption peak toward 583 nm. And the presence of Mie scattering effects at the longer wavelength indicated the formation of larger supramolecular structure. The effect of anions on the above supramolecular structures also followed the order of Hofmeister series, showing that the aggregation of Congo red/β-glucan complex into supramolecular structure was mainly driven through hydrophobic interactions between Congo red micelles on the complex. The research indicated that ions had an important effect on the aggregation of Congo red and the interaction between biopolymers and Congo red.
吴小燕，赵 兰，李琳琳，李 健，王汉源，吴 佳*. 紫外-可见吸收光谱法研究阴离子对刚果红/β-葡聚糖络合物的影响[J]. 光谱学与光谱分析, 2016, 36(11): 3597-3601.
WU Xiao-yan, ZHAO Lan, LI Lin-lin, LI Jian, WANG Han-yuan, WU Jia*. Characterization of the Effect of Anion on the Complex of Oat β-Glucan and Congo Red with UV-Vis Absorption Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(11): 3597-3601.
[1] Purkait M K，Maiti A，DasGupta S，et al. Journal of Hazardous Materials，2007，145(2)：287. [2] Samiey B，Dargahi M R.Central European Journal of Chemistry，2010，8(4)：906. [3] LIN Yu，CHEN Jun-pao，LIU Ling-juan，et al(林 煜，陈俊抛，刘凌娟，等). Chinese Journal of Neurology(中华神经科杂志)，2000，33(4)：207. [4] Barbara S，Barbara P，Leszek K，et al. Acta Biochimica Polonica，2003，4：1213. [5] Lendel C，Bertoncini C W，Cremades N，et al. Biochemistry，2009，48：8322. [6] MEN Rui-zhi，LIU Mei，CHENG Xin-min，et al(门瑞芝，刘 梅，程新民，等). Metallurgical Analysis(冶金分析)，1988，18(3)：5. [7] YANG Yi，GUO Guang-sheng，WANG Zhi-hua，et al(杨 屹，郭广生，王志华，等). Journal of Beijing University of Chemical Technology(北京化工大学学报)，1999，26(3)：73. [8] ZHANG Kai，SUN Qi-zhi，LIANG Hong-wei，et al(张 凯，孙其志，梁宏伟，等). Chinese Journal of Analysis Laboratory(分析试验室)，1997，16(6)：40. [9] LIU Shao-pu，HU Xiao-li，LIU Zhong-fang，et al(刘绍璞，胡小莉，刘忠芳，等). Science in China Series B: Chemistry(中国科学B辑化学)，2006，36(4)：317. [10] Maltsev A S，Grishaev A，Bax A.Biochemistry，2012，51：631. [11] Skowronek M，Stopa B，Konieczny L，et al.Biopolymers，1998，46：267. [12] Wood P J，Fulcher R G，Stone B A.Journal of Cereal Science，1983，1：95. [13] ZHANG Juan，DU Xian-feng，RAO Yan-qin(张 娟，杜先锋，饶砚琴). Journal of Anhui Agricultural University(安徽农业大学学报)，2007，34(1)：23. [14] Spano F C. Accounts of Chemical Research，2010，43(3)：429. [15] Auweter H，Haberkorn H，Heckmann W，et al.Angewandte Chemie International Edition，1999，38(15)：2188.
